We narrowed to 9,174 results for: Pol

1. pCJ203

   Plasmid

   #162678

   Purpose

   pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with deletion of the predicted signal peptide (Phe2:Ala75)

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   Putative MHETase from Comomonas thiooxydans (Genbank WP_080747404.1) without 75 residue signal peptide
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   deltaF2-A75; codon optimized for expression in E.…

   Promoter

   T7/lac

   Available Since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. hu-TLR4 299 snp-flag pDEST40

   Plasmid

   #42647

   Purpose

   destination vector for expression of human TLR4 299 SNP in mammalian cells

   Depositor

   Scott Friedman

   Article

   Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

   Insert

   TLR4 299 SNP (TLR4 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D299G

   Promoter

   CMV

   Available Since

   March 3, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pQCXIB Flag MFF S155A

   Plasmid

   #74385

   Purpose

   Retroviral expression vector containing Flag tagged S155A MFF isoform 5 mutant

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   Flag-MFF (MFF Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ser 155 Ala (numbering based on MFF isoform 1)

   Promoter

   CMV

   Available Since

   May 10, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pQCXIB Flag MFF S172A

   Plasmid

   #74386

   Purpose

   Retroviral expression vector containing Flag tagged S172A MFF isoform 5 mutant

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   Flag-MFF (MFF Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ser 172 Ala (numbering based on MFF isoform 1)

   Promoter

   CMV

   Available Since

   May 10, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-mCherry

   Plasmid

   #122959

   Purpose

   Lentiviral expression of NOTCH2NL delta C terminus under CAG promoter with mCherry expression

   Depositor

   Pierre Vanderhaeghen

   Article

   Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

   Insert

   NOTCH2NL-delta C terminus (NOTCH2NLB Human)
   

   Use

   Lentiviral

   Tags

   6xmyc tag

   Mutation

   deletion of C terminus from full length NOTCH2NLB

   Promoter

   CAG promoter

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-EGFP

   Plasmid

   #122956

   Purpose

   Lentiviral expression of NOTCH2NL delta C terminus under CAG promoter with EGFP expression

   Depositor

   Pierre Vanderhaeghen

   Article

   Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

   Insert

   NOTCH2NL-delta C terminus (NOTCH2NLB Human)
   

   Use

   Lentiviral

   Tags

   6xhis tag

   Mutation

   deletion of C terminus from full length NOTCH2NLB

   Promoter

   CAG promoter

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. LLP245 pEF1a-TALE-DNMT3a-Wt

   Plasmid

   #100937

   Purpose

   Empty TALE vector driven by EF1a promoter, no Puro, contains DNMT3a catalytic domain

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   TALE insertion site, DNMT3a catalytic domain (DNMT3A Synthetic, Human)
   

   Use

   TALEN

   Tags

   3xHA and 3xTy1

   Expression

   Mammalian

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEMS1162

   Plasmid

   #29077

   Purpose

   Expression of the eGFP reporter gene was not detected in the brain and eye.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   Ple94 (GPR88 Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   EGFP-NLS

   Available Since

   Aug. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3 UQCC2(5’UTR)-FF

   Plasmid

   #85487

   Purpose

   Firefly luciferase under the control of UQCC2 5'UTR

   Depositor

   Ivan Topisirovic

   Article

   Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

   Insert

   5'UTR of UQCC2 (C6orf125 Human)
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Stx3 triple mutant (F31A D33A E34A)

    Plasmid

    #86945

    Purpose

    Expresses human Stx3 with triple mutation in the apical targeting signal (F31A D33A E34A)

    Depositor

    Thomas Weimbs

    Article

    Soo Hoo et al PLoS One. 2016 Sep 23;11(9):e0163671. do

    Insert

    STX3 triple mutant (F31A D33A E34A) (STX3 Human)
    

    Tags

    myc-His6

    Expression

    Mammalian

    Mutation

    triple mutant (F31A D33A E34A)

    Available Since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-FLAG-CHD5-Y619E-FRAG

    Plasmid

    #68871

    Purpose

    Mammalian expression of CHD5 histone binding domain (331-672aa) with Y619 mutation

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5 (CHD5 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    331-672aa, Y619E

    Promoter

    CMV

    Available Since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pShuttle-CMV-Flag-SREBP-1c

    Plasmid

    #237348

    Purpose

    Adenoviral-SREBP-1c cleavage-activation reporter system

    Depositor

    Gerald Höfler

    Article

    Pena de la Sancha et al Commun Biol. 2025 Apr 9;8(1):588. doi: 1

    Insert

    SREBF1 (SREBF1 Human)
    

    Use

    Adenoviral and Mouse Targeting

    Tags

    Flag tag

    Promoter

    CMV

    Available Since

    Nov. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF-1α-Venus-Nup107-FRT

    Plasmid

    #247344

    Purpose

    Expresses Venus-NUP107 under EF1 promoter and can be integrated into FRT site

    Depositor

    Kazuhiro Maeshima

    Article

    Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

    Insert

    Nup107 (NUP107) (NUP107 Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Nov. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMAPLe4 PCMTD1 T62 H67 M68

    Plasmid

    #246506

    Purpose

    Recombinant protein expression of PCMTD1 T62 H67 M68

    Depositor

    Steven Clarke

    Article

    Pang et al bioRxiv [Preprint]. 2025 May 21:2025.05.

    Insert

    Protein-L-isoaspartate O-methyltransferase domain-containing protein 1 (PCMTD1 Human)
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Residue 312 is Isoleucine in this plasmid but occ…

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB_rtTA_BsmB-HNRNPK_sgRNA_4

    Plasmid

    #242899

    Purpose

    PiggyBac cargo vector with HNRNPK sgRNA 4 for dox-inducible knockdown

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al Mol Cell. 2019 Aug 8;75(3):523-537.e10.

    Insert

    HNRNPK (Hnrnpk Mouse)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Sept. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB_rtTA_BsmB-HNRNPK_sgRNA_1

    Plasmid

    #242897

    Purpose

    PiggyBac cargo vector with HNRNPK sgRNA 1 for dox-inducible knockdown

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al Mol Cell. 2019 Aug 8;75(3):523-537.e10.

    Insert

    HNRNPK (Hnrnpk Mouse)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB_rtTA_BsmB-HNRNPK_sgRNA_3

    Plasmid

    #242898

    Purpose

    PiggyBac cargo vector with HNRNPK sgRNA 3 for dox-inducible knockdown

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al Mol Cell. 2019 Aug 8;75(3):523-537.e10.

    Insert

    HNRNPK (Hnrnpk Mouse)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-4T-3-GST-Tev-Af1521(K35E/Y145R)-myc

    Plasmid

    #196241

    Purpose

    N-terminal GST fusion of Af1521(K35E/Y145R) with a TEV protease site located between the GST tag and Af1521 and a myc tag on the C terminus

    Depositor

    Robert Sobol

    Article

    Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

    Insert

    N-terminal GST fusion of Af1521(K35E/Y145R) with a TEV protease site between GST and Af1521 encoding a C-terminal myc tag
    

    Tags

    GST tag and myc tag

    Expression

    Bacterial

    Mutation

    amino acid 35 lysine is replaced with glutamic ac…

    Available Since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. TCTN3 minigene (pAVA3939)

    Plasmid

    #239345

    Purpose

    Expresses TCTN3 minigene (EFS-6xMyc-TCTN3-Ex10-I10-Ex11-copGFP-3xFLAG)

    Depositor

    Andrei Alexandrov

    Article

    Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

    Insert

    EFS-6xMyc-TCTN3-Ex10-I10-Ex11-copGFP-3xFLAG-BGH polyA (TCTN3 Human)
    

    Tags

    3xFLAG, 6xMyc, and CopGFP

    Expression

    Mammalian

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. Rab11 single guide RNA

    Plasmid

    #229683

    Purpose

    guide RNA for Rab11 tagging at the N-terminus in pX459

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    Rab11 guide (RAB11A Human)
    

    Use

    CRISPR

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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