Ubiquiton-An inducible, linkage-specific polyubiquitylation tool.
Renz C, Asimaki E, Meister C, Albanese V, Petriukov K, Krapoth NC, Wegmann S, Wollscheid HP, Wong RP, Fulzele A, Chen JX, Leon S, Ulrich HD
Mol Cell. 2024 Jan 18;84(2):386-400.e11. doi: 10.1016/j.molcel.2023.11.016. Epub 2023 Dec 15. PubMed Article
Renz C, Asimaki E, Meister C, Albanese V, Petriukov K, Krapoth NC, Wegmann S, Wollscheid HP, Wong RP, Fulzele A, Chen JX, Leon S, Ulrich HD
Mol Cell. 2024 Jan 18;84(2):386-400.e11. doi: 10.1016/j.molcel.2023.11.016. Epub 2023 Dec 15. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 212711 | pET22b-His6-UBCH7 | Bacterial expression of His6-UBCH7 |
| 212712 | pFastBac1-His6-E3(1)-CUbo-TwinStrep | Expression of His6-E3(1)-CUbo-TwinStrep in insect cells |
| 212713 | pMAL-TEV2-Ubc7-His6 | Bacterial expression of MBP-Ubc7-His6, MBP-tag cleavable with TEV-protease |
| 212714 | pET24a-NUbo-E3(48)-His6 | Bacterial expression of NUbo-E3(48)-His6 |
| 212715 | pET21(+)-His6-Ubc13 | Bacterial expression of His6-Ubc13 |
| 212716 | pET30-His6-TEV-Mms2 | Bacterial expression of His6-Mms2, His6-tag cleavable with TEV-protease |
| 212717 | pET24a-NUbo-E3(63)-His6 | Bacterial expression of NUbo-E3(63)-His6 |
| 212718 | pET24a-NUbo-GFP-His6 | Bacterial expression of NUbo-GFP-His6 |
| 212719 | pET24a-His7-NUbo-GFP | Bacterial expression of His7-NUbo-GFP |
| 212720 | pET15b-His6-CUbo-GFP | Bacterial expression of His6-CUbo-GFP |
| 212721 | pET15b-His6-CUbo(K48R)-GFP | Bacterial expression of His6-CUbo(K48R)-GFP |
| 212722 | pET15b-His6-CUbo(K63R)-GFP | Bacterial expression of His6-CUbo(K63R)-GFP |
| 212723 | pET24a-NUbo-MCS-His6 | Bacterial expression of a protein of interest with N-terminal NUbo- and C-terminal His6-fusions, contains multiple cloning site |
| 212724 | pET24a-His7-NUbo-MCS | Bacterial expression of a protein of interest with N-terminal His7-NUbo-fusion, contains multiple cloning site |
| 212725 | pET24a-His6-CUbo-MCS | Bacterial expression of a protein of interest with N-terminal His6-CUbo-fusion, contains multiple cloning site |
| 212726 | pET24a-His6-CUbo(K48R)-MCS | Bacterial expression of a protein of interest with N-terminal His6-CUbo(K48R)-fusion, contains multiple cloning site |
| 212727 | pET24a-His6-CUbo(K63R)-MCS | Bacterial expression of a protein of interest with N-terminal His6-CUbo(K63R)-fusion, contains multiple cloning site |
| 212728 | YIp128-ADH-NUbo-VSV | Consitutive expression of NUbo-VSV in budding yeast |
| 212729 | YIp211-ADH-NUbo-VSV | Consitutive expression of NUbo-VSV in budding yeast |
| 212730 | YIp211-CUP1-NUbo-VSV | Copper-inducible expression of NUbo-VSV in budding yeast |
| 212731 | YIp128-ADH-NUbo-mCherry-VSV | Consitutive expression of NUbo-mCherry-VSV in budding yeast |
| 212732 | YIp128-ADH-CUbo-VSV | Consitutive expression of CUbo-VSV in budding yeast |
| 212733 | YIp211-ADH-CUbo-VSV | Consitutive expression of CUbo-VSV in budding yeast |
| 212734 | YIp128-ADH-CUbo-mCherry-VSV | Consitutive expression of CUbo-mCherry-VSV in budding yeast |
| 212735 | YIp128-ADH-myc-E3(1)-CUbo-VSV | Consitutive expression of myc-E3(1)-CUbo-VSV in budding yeast |
| 212736 | YIp211-ADH-myc-E3(1)-CUbo-VSV | Consitutive expression of myc-E3(1)-CUbo-VSV in budding yeast |
| 212737 | YIp128-ADH-myc-E3(1)-CUbo-NLS-VSV | Consitutive expression of myc-E3(1)-CUbo-NLS-VSV in budding yeast |
| 212738 | YIp211-ADH-myc-E3(1) C885A-CUbo-VSV | Consitutive expression of myc-E3(1) C885A-CUbo-VSV in budding yeast, catalytic inactive E3(1) |
| 212739 | YIp128-ADH-myc-E3(1)-CUbo-mCherry-VSV | Consitutive expression of myc-E3(1)-CUbo-mCherry-VSV in budding yeast |
| 212740 | YIp211-ADH-myc-E3(1)-CUbo-mCherry-VSV | Consitutive expression of myc-E3(1)-CUbo-mCherry-VSV in budding yeast |
| 212741 | YIp128-ADH-NUbo-E3(48)-VSV | Consitutive expression of NUbo-E3(48)-VSV in budding yeast |
| 212742 | YIp211-ADH-NUbo-E3(48)-VSV | Consitutive expression of NUbo-E3(48)-VSV in budding yeast |
| 212743 | YIp128-TET-NUbo-E3(48)-VSV | Doxycycline-inducible expression of NUbo-E3(48)-VSV in budding yeast |
| 212744 | YIp211-TET-NUbo-E3(48)-VSV | Doxycycline-inducible expression of NUbo-E3(48)-VSV in budding yeast |
| 212745 | YIp128-ADH-NUbo-E3(48)-NLS-VSV | Constitutive expression of NUbo-E3(48)-NLS-VSV in budding yeast |
| 212746 | YIp128-TET-NUbo-E3(48)-NLS-VSV | Doxycycline-inducible expression of NUbo-E3(48)-NLS-VSV in budding yeast |
| 212747 | YIp211-ADH-NUbo-E3(48) aa24-150-VSV | Constitutive expression of NUbo-E3(48)ΔU7BD-VSV in budding yeast, inactive E3(48) |
| 212748 | YIp128-ADH-NUbo-E3(48)-mCherry-VSV | Constitutive expression of NUbo-E3(48)-mCherry-VSV in budding yeast |
| 212749 | YIp211-ADH-NUbo-E3(48)-mCherry-VSV | Constitutive expression of NUbo-E3(48)-mCherry-VSV in budding yeast |
| 212750 | YIp128-ADH-NUbo-E3(63)-VSV | Constitutive expression of NUbo-E3(63)-VSV in budding yeast |
| 212751 | YIp211-ADH-NUbo-E3(63)-VSV | Constitutive expression of NUbo-E3(63)-VSV in budding yeast |
| 212752 | YIp128-TET-NUbo-E3(63)-VSV | Doxycycline-inducible expression of NUbo-E3(63)-VSV in budding yeast |
| 212753 | YIp211-TET-NUbo-E3(63)-VSV | Doxycycline-inducible expression of NUbo-E3(63)-VSV in budding yeast |
| 212754 | YIp211-CUP1-NUbo-E3(63)-VSV | Copper-inducible expression of NUbo-E3(63)-VSV in budding yeast |
| 212755 | YIp128-ADH-NUbo-E3(63)-NLS-VSV | Constitutive expression of NUbo-E3(63)-NLS-VSV in budding yeast |
| 212756 | YIp128-TET-NUbo-E3(63)-NLS-VSV | Doxycycline-inducible expression of NUbo-E3(63)-NLS-VSV in budding yeast |
| 212757 | YIp211-CUP1-NUbo-E3(63) I227A-VSV | Copper-inducible expression of NUbo-E3(63) I227A-VSV in budding yeast, reduced activity of E3(63) |
| 212758 | YIp128-ADH-NUbo-E3(63)-mCherry-VSV | Constitutive expression of NUbo-E3(63)-mCherry-VSV in budding yeast |
| 212759 | YIp211-ADH-NUbo-E3(63)-mCherry-VSV | Constitutive expression of NUbo-E3(63)-mCherry-VSV in budding yeast |
| 212760 | pUra3-pCUP1-NUbo | N-terminal PCR-based tagging with pCUP1-NUbo in budding yeast with URA3 selection |
| 212761 | pUra3-pCUP1-His7-NUbo | N-terminal PCR-based tagging with pCUP1-His7-NUbo in budding yeast with URA3 selection |
| 212762 | pUra3-pCUP1-CUbo | N-terminal PCR-based tagging with pCUP1-CUbo in budding yeast with URA3 selection |
| 212763 | pUra3-pCUP1-CUbo(K48R) | N-terminal PCR-based tagging with pCUP1-CUbo(K48R) in budding yeast with URA3 selection |
| 212764 | pUra3-pCUP1-CUbo(K63R) | N-terminal PCR-based tagging with pCUP1-CUbo(K63R) in budding yeast with URA3 selection |
| 212765 | pUra3-pCUP1-CUbo(K48R/K63R) | N-terminal PCR-based tagging with pCUP1-CUbo(K48R/K63R) in budding yeast with URA3 selection |
| 212766 | pHyg-CUbo-yeGFP | C-terminal PCR-based tagging with CUbo-yeGFP in budding yeast with HygR selection |
| 212767 | pHyg-CUbo(K48R)-yeGFP | C-terminal PCR-based tagging with CUbo(K48R)-yeGFP in budding yeast with HygR selection |
| 212768 | pHyg-CUbo(K63R)-yeGFP | C-terminal PCR-based tagging with CUbo(K63R)-yeGFP in budding yeast with HygR selection |
| 212769 | pHyg-CUbo(K48R/K63R)-yeGFP | C-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP in budding yeast with HygR selection |
| 212770 | pHyg-CUbo-AID*-GFP | C-terminal PCR-based tagging with CUbo-AID*-yeGFP in budding yeast with HygR selection |
| 212771 | pCaUra3-CUbo-yeGFP | C-terminal PCR-based tagging with CUbo-yeGFP in budding yeast with CaUra3 selection |
| 212772 | pCaUra3-CUbo(K48R)-yeGFP | C-terminal PCR-based tagging with CUbo(K48R)-yeGFP in budding yeast with CaUra3 selection |
| 212773 | pCaUra3-CUbo(K63R)-yeGFP | C-terminal PCR-based tagging with CUbo(K63R)-yeGFP in budding yeast with CaUra3 selection |
| 212774 | pCaUra3-CUbo(K48R/K63R)-yeGFP | C-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP in budding yeast with CaUra3 selection |
| 212775 | pCaUra3-CUbo-AID*-GFP | C-terminal PCR-based tagging with CUbo-AID*-yeGFP in budding yeast with CaUra3 selection |
| 212776 | pHyg-His6 | C-terminal PCR-based tagging with His6 in budding yeast with HygR selection |
| 212777 | pHyg-CUbo-His6 | C-terminal PCR-based tagging with CUbo-His6 in budding yeast with HygR selection |
| 212778 | pHyg-CUbo(K48R)-His6 | C-terminal PCR-based tagging with CUbo(K48R)-His6 in budding yeast with HygR selection |
| 212779 | pHyg-CUbo(K63R)-His6 | C-terminal PCR-based tagging with CUbo(K63R)-His6 in budding yeast with HygR selection |
| 212780 | pHyg-CUbo(K48R/K63R)-His6 | C-terminal PCR-based tagging with CUbo(K48R/K63R)-His6 in budding yeast with HygR selection |
| 212781 | pHyg-yeGFP-His6 | C-terminal PCR-based tagging with yeGFP-His6 in budding yeast with HygR selection |
| 212782 | pHyg-CUbo-yeGFP-His6 | C-terminal PCR-based tagging with CUbo-yeGFP-His6 in budding yeast with HygR selection |
| 212783 | pHyg-CUbo(K48R)-yeGFP-His6 | C-terminal PCR-based tagging with CUbo(K48R)-yeGFP-His6 in budding yeast with HygR selection |
| 212784 | pHyg-CUbo(K63R)-yeGFP-His6 | C-terminal PCR-based tagging with CUbo(K63R)-yeGFP-His6 in budding yeast with HygR selection |
| 212785 | pHyg-CUbo(K48R/K63R)-yeGFP-His6 | C-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP-His6 in budding yeast with HygR selection |
| 212786 | pHyg-CUbo-9myc | C-terminal PCR-based tagging with CUbo-9myc in budding yeast with HygR selection |
| 212787 | pHyg-CUbo(K48R)-9myc | C-terminal PCR-based tagging with CUbo(K48R)-9myc in budding yeast with HygR selection |
| 212788 | pHyg-CUbo(K63R)-9myc | C-terminal PCR-based tagging with CUbo(K63R)-9myc in budding yeast with HygR selection |
| 212789 | pHyg-CUbo(K48R/K63R)-9myc | C-terminal PCR-based tagging with CUbo(K48R/K63R)-9myc in budding yeast with HygR selection |
| 212790 | YIp128-CUP1-NUbo-GFP-His6 | Copper-inducible expression of the artificial test substrate NUbo-GFP-His6 in budding yeast |
| 212791 | YIp128-CUP1-His7-NUbo-GFP | Copper-inducible expression of the artificial test substrate His7-NUbo-GFP in budding yeast |
| 212792 | YIp128-CUP1-His6-CUbo-GFP | Copper-inducible expression of the artificial test substrate His6-CUbo-GFP in budding yeast |
| 212793 | YIp128-CUP1-His6-CUbo(K48R)-GFP | Copper-inducible expression of the artificial test substrate His6-CUbo(K48R)-GFP in budding yeast |
| 212794 | YIp128-CUP1-His6-CUbo(K63R)-GFP | Copper-inducible expression of the artificial test substrate His6-CUbo(K63R)-GFP in budding yeast |
| 212795 | YIp128-CUP1-His6-CUbo(K48R/K63R)-GFP | Copper-inducible expression of the artificial test substrate His6-CUbo(K48R/K63R)-GFP in budding yeast |
| 212796 | YIp128-CUP1-CUbo-AID*-GFP | Copper-inducible expression of the artificial test substrate His6-CUbo-AID*-GFP in budding yeast |
| 212797 | pcDNA5-FRT-TO-NUbo-FLAG | Constitutive/Doxycycline-inducible expression of NUbo-FLAG in mammalian cells |
| 212798 | pcDNA5-FRT-TO-NUbo-mCherry-FLAG | Constitutive/Doxycycline-inducible expression of NUbo-mCherry-FLAG in mammalian cells |
| 212799 | pcDNA5-FRT-TO-Myc-E3(1)-CUbo | Constitutive/Doxycycline-inducible expression of Myc-E3(1)-CUbo in mammalian cells |
| 212800 | pcDNA5-FRT-TO-Myc-E3(1) C885A-CUbo | Constitutive/Doxycycline-inducible expression of Myc-E3(1) C885A-CUbo in mammalian cells |
| 212801 | pDEST-3xFLAG-Ubc7 | Constitutive expression of 3xFLAG-tagged budding yeast Ubc7 in mammalian cells |
| 212802 | pcDNA5-FRT-TO-NUbo-E3(48)-FLAG | Constitutive/Doxycycline-inducible expression of NUbo-E3(48)-FLAG in mammalian cells |
| 212803 | pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-FLAG | Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-FLAG in mammalian cells |
| 212804 | pcDNA5-FRT-TO-NUbo-E3(48)-P2A-3xFLAG-Ubc7 | Constitutive or doxycycline-inducible expression of NUbo-E3(48)-P2A-3xFLAG-Ubc7 in mammalian cells |
| 212805 | pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7 | Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7 in mammalian cells |
| 212806 | pcDNA5-FRT-TO-Ubc13 | Constitutive or doxycycline-inducible expression of budding yeast Ubc13 in mammalian cells |
| 212807 | pcDNA5-FRT-TO-Mms2 | Constitutive or doxycycline-inducible expression of budding yeast Mms2 in mammalian cells |
| 212808 | pcDNA5-FRT-TO-NUbo-E3(63)-FLAG | Constitutive or doxycycline-inducible expression of NUbo-E3(63)-FLAG in mammalian cells |
| 212809 | pcDNA5-FRT-TO-NUbo-E3(63)-mCherry-FLAG | Constitutive or doxycycline-inducible expression of NUbo-E3(63)-mCherry-FLAG in mammalian cells |
| 212810 | pcDNA5-FRT-TO-NUbo-H2B-His8 | Constitutive or doxycycline-inducible expression of NUbo-H2B-His8 in mammalian cells |
| 212811 | pcDNA5-FRT-TO-His6-NUbo-H2B-His8 | Constitutive or doxycycline-inducible expression of His6-NUbo-H2B-His8 in mammalian cells |
| 212812 | pcDNA5-FRT-TO-His8-H2B-CUbo | Constitutive or doxycycline-inducible expression of His8-H2B-CUbo in mammalian cells |
| 212813 | pcDNA5-FRT-TO-His8-H2B-CUbo(K48R) | Constitutive or doxycycline-inducible expression of His8-H2B-CUbo(K48R) in mammalian cells |
| 212814 | pcDNA5-FRT-TO-His8-H2B-CUbo(K63R) | Constitutive or doxycycline-inducible expression of His8-H2B-CUbo(K63R) in mammalian cells |
| 212815 | pcDNA5-FRT-TO-EGFR*-CUbo-GFP | Constitutive or doxycycline-inducible expression of EGFR*-CUbo-GFP in mammalian cells |
| 212816 | pcDNA5-FRT-TO-EGFR*-CUbo(K48R)-GFP | Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K48R)-GFP in mammalian cells |
| 212817 | pcDNA5-FRT-TO-EGFR*-CUbo(K63R)-GFP | Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K63R)-GFP in mammalian cells |
| 212818 | pcDNA5-FRT-TO-EGFR*-CUbo(K48R K63R)-GFP | Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K48R K63R)-GFP in mammalian cells |
| 212819 | pcDNA5-FRT-TO-EGFR*-CUbo-His6 | Constitutive or doxycycline-inducible expression of EGFR*-CUbo-His6 in mammalian cells |
| 212820 | pcDNA5-FRT-TO-EGFR*-CUbo(K63R)-His6 | Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K63R)-His6 in mammalian cells |
| 212821 | pcDNA5-FRT-TO-EGFR*-CUbo | Constitutive or doxycycline-inducible expression of EGFR*-CUbo in mammalian cells |
| 212822 | pcDNA5-FRT-TO-EGFR*-CUbo(K63R) | Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K63R) in mammalian cells |