We narrowed to 6,955 results for: CAR
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tetO-HAND1
Plasmid#170689Purposedoxycycline-inducible overexpression of HAND1DepositorInsertHAND1 (HAND1 Human)
UseLentiviral; Doxycycline inducibleExpressionMammalianPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-FHIP
Plasmid#198527PurposeExpression of GAL4 DNA-binding domain (BD)-FHIP fusion protein in yeast (yeast two-hybrid assays)DepositorInsertFHIP (FHIP1B Human)
TagsGAL4-DNA binding domain fragmentExpressionYeastMutationsilent mutations in codons 353 and 651 as well as…PromoterADH1Available sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-NLS-YFP-CCTc
Plasmid#184327PurposeMammalian expression of the nuclear localized YFP-CCTd(peptide of Cav1.2)DepositorInsertCCTc (CACNA1C Human)
ExpressionMammalianAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
t1-405(T144E,T150E)
Plasmid#166130PurposeFor expression of human talin-1 head (residues 1-405) in E. coli. Includes mutations T144E and T150E. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.DepositorInsertHuT1head1-405(T144E,T150E) (TLN1 Human)
TagsHis6-tag, Xpress-epitope (DLYDDDDK) and enterokin…ExpressionBacterialMutationresidues 1-405, mutations T144E and T150EAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE
Plasmid#166805PurposeMammalian cell expression vector for FLAG-tagged NLRP1. The LL1193E mutant abolishes FL-NLRP1 association with DPP9 at interface I.DepositorAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 LIC 6A NLRP1-FLAG K1277E
Plasmid#166808PurposeMammalian cell expression vector for FLAG-tagged NLRP1. The K1277E mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activityDepositorAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 LIC 6A NLRP1-FLAG H1276G
Plasmid#166807PurposeMammalian cell expression vector for FLAG-tagged NLRP1. The H1276G mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activityDepositorAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 LIC 6A NLRP1-FLAG P1214R
Plasmid#166806PurposeMammalian cell expression vector for FLAG-tagged NLRP1. The P1214R patient mutant abolishes NLRP1-CT association with DPP9 at interface II.DepositorAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hYAP1-Y1K)-PGKpuro2ABFP-W
Plasmid#163170PurposeLentiviral gRNA plasmid targeting human YAP1 gene, co-expression of BFP tagDepositorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hYAP1-Y1K)-PGKpuro2AmCherry-W
Plasmid#163172PurposeLentiviral gRNA plasmid targeting human YAP1 gene, co-expression of mCherry tagDepositorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
prCOrev
Plasmid#153961PurposeUsed to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)DepositorInsert5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the opposite direction
UseRetroviral; A reporter plasmid detecting dna reco…TagsNLS-ZFExpressionMammalianMutationNo mutationPromoterLTRAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
prCO
Plasmid#153960PurposeUsed to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)DepositorInsert5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the same direction
UseRetroviral; A reporter plasmid detecting dna reco…TagsNLS-ZFExpressionMammalianMutationNo mutationPromoterLTRAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Halo-Tev- HSPB7 (zebrafish) C117S
Plasmid#108915PurposeExpress C117S mutant of Zebrafish Halo-Tev-HSPB7 in mammalian cellsDepositorInsertHalo-TEV-HSPB7
TagsHalo-TEVExpressionMammalianMutationCysteine 117 to SerinePromoterSP6Available sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3 SIRT1-NTerm Exon1-Exon3
Plasmid#105681PurposeBacterial Expression construct of N terminus of SIRT1: Exon1-Exon3 (deltaExon2)DepositorInsertSIRT1-NTerm: Exon1-Exon3 (Sirt1 Mouse)
TagsGSTExpressionBacterialMutationSynonymous base changes not affecting the protein…Promotertac_PromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-RP2-C86Y,P95L
Plasmid#86074PurposeA bacterial expression plasmid encoding ciliary localization effected RP2 (RP2-C86Y,P95L) with N-terminus GST-tag.DepositorInsertRP2 (RP2 Human)
TagsGSTExpressionBacterialMutationChanged cysteine-86 and proline-95 to tyrosine an…PromotertacAvailable sinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
FPGT-TNNI3K gRNA (BRDN0001145543)
Plasmid#75902Purpose3rd generation lentiviral gRNA plasmid targeting human FPGT-TNNI3KDepositorInsertFPGT-TNNI3K,TNNI3K (FPGT-TNNI3K Human)
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FPGT-TNNI3K gRNA (BRDN0001147862)
Plasmid#75903Purpose3rd generation lentiviral gRNA plasmid targeting human FPGT-TNNI3KDepositorInsertFPGT-TNNI3K,TNNI3K (FPGT-TNNI3K Human)
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FPGT-TNNI3K gRNA (BRDN0001147836)
Plasmid#75904Purpose3rd generation lentiviral gRNA plasmid targeting human FPGT-TNNI3KDepositorInsertFPGT-TNNI3K,TNNI3K (FPGT-TNNI3K Human)
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FPGT-TNNI3K gRNA (BRDN0001148036)
Plasmid#75905Purpose3rd generation lentiviral gRNA plasmid targeting human FPGT-TNNI3KDepositorInsertFPGT-TNNI3K,TNNI3K (FPGT-TNNI3K Human)
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only
