We narrowed to 9,174 results for: Pol

1. mCherry-PARP1

   Plasmid

   #215136

   Purpose

   Expresses mCherry-PARP1 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-CAG-mRuby3-Gal8-P2A-Zeo

   Plasmid

   #150815

   Purpose

   Mammalian expression of mRuby3-Galectin8 (Gal8) fusion protein

   Depositor

   Jordan Green

   Article

   Rui et al Sci Adv. 2022 Jan 7;8(1):eabk2855. doi:

   Insert

   mRuby3-Gal8-P2A-Zeo (LGALS8 Human, Synthetic)
   

   Tags

   Gal8 is fused to the c-terminus of mRuby3

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBabe-Puro-MEK-DD

   Plasmid

   #15268

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   Map2k1 (Mapk1 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   S218D S222D

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a_His-TEV-EiCsm6

   Plasmid

   #172487

   Purpose

   Bacterial expression of codon-optimized His-EiCsm6 with a TEV protease cleavage site.

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

   Insert

   Enterococcus italicus Csm6 (codon optimized)
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7 promoter and lac operator

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21a(+)-HIV RT-6xHis-RBS-prot

   Plasmid

   #159149

   Purpose

   expresses His-tagged HIV reverse transcriptase and untagged HIV protease

   Depositor

   Andrea Pauli

   Article

   Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

   Inserts

   Human immunodeficiency virus 1 (HIV-1) reverse transcriptase
   Human immunodeficiency virus 1 (HIV-1) protease
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   HIV-1 group M/subtype B – BH10 strain

   Available Since

   Sept. 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pEGFP-N1-PARP1-E988K

   Plasmid

   #211580

   Purpose

   CMV driven PARP1-E988K -eGFP expressing construct with IRES-Neomycin selection

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Contains E988K mutation

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac1-pGC-A

   Plasmid

   #186626

   Purpose

   pFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)

   Depositor

   Debra Hansen

   Article

   Zhang et al Sci Rep. 2022 Jul 12;12(1):11824. doi: 1

   Insert

   NPR1 (NPR1 Human)
   

   Tags

   hemagglutinin (HA) signal peptide + His10-tag + T…

   Expression

   Insect

   Mutation

   deleted amino acids 1-32

   Promoter

   polyhedring

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-PARP1_D770A-mCherry

   Plasmid

   #192260

   Purpose

   Expresses PARP1 D770A mutant in mammalian cells. Tagged with mCherry.

   Depositor

   Michael Cohen

   Article

   Arnold et al Cell Chem Biol. 2022 Dec 15;29(12):1694-

   Insert

   PARP1_D770A (PARP1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   D770A

   Promoter

   CMV

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-PARP1_R878A-mCherry

   Plasmid

   #192261

   Purpose

   Expresses PARP1 R878A mutant in mammalian cells. Tagged with mCherry.

   Depositor

   Michael Cohen

   Article

   Arnold et al Cell Chem Biol. 2022 Dec 15;29(12):1694-

   Insert

   PARP1_R878A (PARP1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   R878A

   Promoter

   CMV

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-PARP1-del.p119K120S–eGFP

    Plasmid

    #211554

    Purpose

    A piggyBac vector containing CMV-PARP1-del.p119K120S-eGFP-IRES-Neo cassette.

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Piggybac

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    deletion of amino acids 119K 120S

    Promoter

    CMV

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-N1-PARP1-delM43;F44I

    Plasmid

    #211579

    Purpose

    CMV driven PARP1-delM43;F44I -eGFP expressing construct with IRES-Neomycin selection

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    PARP1 (PARP1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Contains deletion of M43 and F44I

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

    Plasmid

    #67977

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE
    

    Use

    Lentiviral

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWZL Neo Myr Flag SRPK2

    Plasmid

    #20525

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    SRPK2 (SRPK2 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Available Since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.2 TDP-43 NLS1 YFP

    Plasmid

    #84912

    Purpose

    Mammalian expresion of TDP-43 NLS1 YFP

    Depositor

    Aaron Gitler

    Article

    Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    K82A, R83A, K84A

    Promoter

    CMV

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWZL Neo Myr Flag YES1

    Plasmid

    #20665

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    YES1 (YES1 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Available Since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pCJ217

    Plasmid

    #162685

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating two point mutations, G489C and S530C, to introduce a 6th disulfide bond (from PETase).

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with mutations to introduce a 6th disulfide bond from PETase
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    G489C, S530C; codon optimized for E. coli K12

    Promoter

    T7/lac

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W

    Plasmid

    #67979

    Purpose

    Cas9 activity reporter (control) with BFP and GFP.

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
    

    Use

    Lentiviral

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGJK_His-SUMO-LbuCas13a

    Plasmid

    #172488

    Purpose

    Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

    Insert

    bdSUMO-LbuCas13a (codon-optimized)
    

    Tags

    His6 and bdSUMO

    Expression

    Bacterial

    Promoter

    T7 promoter and lac operator

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. TRIPZ-bioGEF-UBnc-PURO

    Plasmid

    #208044

    Purpose

    Enables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    UBnc (UBC Human)
    

    Use

    Lentiviral

    Tags

    Low-affinity Avitag

    Mutation

    L73P Mutation near C-terminus suppresses cleavage…

    Promoter

    tetO/UBC

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W

    Plasmid

    #67981

    Purpose

    Cas9 activity reporter (control) with mCherry and GFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Mutation

    Deleted BbsI site within WPRE

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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