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pEG-MeCP2_R294X (pc4753)

Plasmid

#248580

Purpose

Mammalian expression plasmid of MeCP2-R294X (aa1- aa293)

Depositor

Cristina Cardoso

Publication

Romero et al Commun Biol. 2025 Dec 8. doi: 10.1038/s4

Insert

MeCP2 (MECP2 Human)

Tags

GFP

Expression

Mammalian

Mutation

aa1-aa293

Promoter

CMV

Available since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pZucchini2_SFFV-RXRA-eGFP-IRES-mCherry_PuroR

Plasmid

#247929

Purpose

Overexpression vector for eGFP tagged RXRA or NR2B1. Monitor post-translational degradation of RXRA via EGFP:mCherry ratio. Contains puromycin-resistance selection marker.

Depositor

Benjamin Ebert, Jonathan Tsai

Publication

Tsai et al Mol Cell. 2023 Aug 3;83(15):2753-2767.e1

Insert

Retinoid X receptor alpha (RXRA Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Available since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-9-RECK ΔCRD

Plasmid

#246708

Purpose

Expression vector for human RECK lacking the CRD, N-terminal 3xFLAG tag

Depositor

Calvin Kuo, Mario Vallon

Publication

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

RECK (RECK Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Δaa 343-476

Promoter

CMV

Available since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Igκ SP-sRECK-His

Plasmid

#246688

Purpose

Expression vector for secreted human RECK, C-terminal 6xHis tag

Depositor

Calvin Kuo, Mario Vallon

Publication

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

RECK (RECK Human)

Tags

6xHis

Expression

Mammalian

Mutation

ΔGPI anchor signal

Promoter

CMV

Available since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Igκ SP-sGPR124-Fc

Plasmid

#246684

Purpose

Expression vector for a secreted human GPR124 ECD-mouse IgG2a Fc fusion protein

Depositor

Calvin Kuo, Mario Vallon

Publication

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

mouse IgG2a Fc

Expression

Mammalian

Mutation

ECD

Promoter

CMV

Available since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pZucchini2_SFFV-ESR1-eGFP-IRES-mCherry_PuroR

Plasmid

#247930

Purpose

Overexpression vector for eGFP tagged ESR1 or ERα. Monitor post-translational degradation of ERα via EGFP:mCherry ratio. Contains puromycin-resistance selection marker.

Depositor

Benjamin Ebert, Jonathan Tsai

Publication

Tsai et al Mol Cell. 2023 Aug 3;83(15):2753-2767.e1

Insert

Estrogen Receptor Alpha (ESR1 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Available since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-hU6-FOXP2_sgRNA1-hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#231022

Purpose

All-in-one lentiviral vector (Gersbach lab design) for CRISPRi targeting FOXP2

Depositor

Tomasz Nowakowski

Publication

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

FOXP2 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-hU6-FOXP2_sgRNA2-hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#231023

Purpose

All-in-one lentiviral vector (Gersbach lab design) for CRISPRi targeting FOXP2

Depositor

Tomasz Nowakowski

Publication

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

FOXP2 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-hU6-ROBO2_sgRNA-hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#231024

Purpose

All-in-one lentiviral vector (Gersbach lab design) for CRISPRi targeting ROBO2

Depositor

Tomasz Nowakowski

Publication

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

ROBO2 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-hU6-NTC_sgRNA-hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#231025

Purpose

All-in-one lentiviral vector (Gersbach lab design) for CRISPRi with non-targeting control sgRNA

Depositor

Tomasz Nowakowski

Publication

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

Non-targeting control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-CG6867-sfGFP

Plasmid

#240237

Purpose

Expression of CG6867-sfGFP under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Publication

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

CG6867 (CG6867 Fly)

Tags

sfGFP

Expression

Insect

Promoter

UAS

Available since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-mCGN-mCGNL1-Rod1-Myc

Plasmid

#236526

Purpose

For expression of chimeric CGN containing the Rod1 domain from CGNL1 (mouse) in mammalian cells

Depositor

Sandra Citi

Publication

Flinois et al J Cell Sci. 2024 Mar 1;137(5):jcs260745.

Insert

CGN-CGNL1 (Cgn Mouse)

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2puro-p120catenin(Canis)-exon4 116-138 gRNA

Plasmid

#209923

Purpose

A knock-out vector for the dog CTNND1

Depositor

Michiyuki Matsuda

Publication

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

A gRNA targeting the dog CTNND1 gene.

Use

CRISPR and Lentiviral

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pSN667

Plasmid

#219963

Purpose

Expressed sfGFP fused human KIF1Bß(1-721) in insect cells

Depositor

Shinsuke Niwa

Insert

codon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)

Tags

sfGFP::2xStrepII tag

Expression

Insect

Mutation

encodes 1-721

Promoter

polyhedrin

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_DCAF8L2:1-631

Plasmid

#211239

Purpose

Mammalian expression of full-length human DCAF8L2. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF8L2:M1-S631 (DCAF8L2 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

145-147: missing in Poly E region

Promoter

CMV

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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MBP-3C-hTspan12LEL(118-218)_pAcGP67a

Plasmid

#216383

Purpose

Baculovirus transfer vector to secrete human Tspan12 LEL (residues 118-218) fused to MBP

Depositor

William Weis

Publication

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Tetraspanin-12 (TSPAN12 Human)

Use

Baculovirus

Tags

GP64 signal sequence-MBP-3C

Expression

Insect

Promoter

Polyhedrin

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFBOH-LIC_MAGEA3:104-314

Plasmid

#210941

Purpose

Insect expression of human MAGEA3 fragment E104 to E314. Untagged.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

MAGEA3:E104-E314 (MAGEA3 Human)

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_SHKBP1:182-585

Plasmid

#210935

Purpose

Insect expression of human SHKBP1 fragment T182 to L585. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SHKBP1:T182-L585 (SHKBP1 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

Q507L natural variant (VAR_036714)

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_TRAF7:382-670

Plasmid

#210930

Purpose

Insect expression of human TRAF7 fragment P382 to C670. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

TRAF7:P382-C670 (TRAF7 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_TLE3:472-772

Plasmid

#210928

Purpose

Insect expression of human TLE3 fragment G472 to Y772. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

TLE3:G472-Y772 (TLE3 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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