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pEN_TGmiR_Gb2-K

Plasmid

#25766

Purpose

Entry vector withTRE promoter driving mouse G beta 2 miR30-based shRNA with GFP coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Gb2 miR-shRNA (Gnb2 Mouse)

Use

Entry vector

Tags

GFP

Expression

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR221-ARL1

Plasmid

#67316

Purpose

hArl1 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL1 (ARL1 Human)

Use

Gateway entry vector

Tags

Expression

Bacterial

Mutation

bp 231 C to T; silent mutation

Promoter

None

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
D998-EGFR-ET1

Plasmid

#44186

Depositor

Timothy Springer

Article

Mi et al Biochemistry. 2008 Sep 30;47(39):10314-2

Insert

D998-EGFR-ET1 (EGFR Human)

Use

Tags

3C cleavge site, protein C peptide tag, his6, SBP…

Expression

Mammalian

Mutation

EGFR amino acids 1−998

Promoter

CVM

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO/myc-His-A-YAP2-1st&2nd WW mut

Plasmid

#19061

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Jul 17. ():.

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Tryptophan 199 changed to Alanine,and Proline 202…

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA6-E2F4CA-Myc

Plasmid

#80428

Purpose

Mammalian expression of constitutively active chick E2F4

Depositor

José María Frade

Article

Morillo et al Mol Cell Biol. 2012 Jul;32(14):2722-37.

Insert

E2F transcription factor 4 (E2F4 Chicken)

Use

Tags

Myc

Expression

Mammalian

Mutation

T261E, T263E

Promoter

CMV

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221-ARFRP1-no stop

Plasmid

#67343

Purpose

hArfrp1 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARFRP1 (ARFRP1 Human)

Use

Gateway entry vector

Tags

Expression

Bacterial

Mutation

Promoter

None

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pENTR1A dn-cHSF1

Plasmid

#134724

Purpose

Gateway entry vector for constitutively active dominant-negative HSF1

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

dn-cHSF1 (HSF1 Human)

Use

Entry vector for gateway cloning

Tags

Expression

Mutation

Deletion of amino acids 186-202, 379−529

Promoter

None

Available since

July 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/HisMaxB-YAP2-1st&2nd WW mutant

Plasmid

#19000

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Jul 17. ():.

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

His

Expression

Mammalian

Mutation

Tryptophan 199 changed to Alanine, and Proline 2…

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pMX_hTAF4_Cterminus

Plasmid

#44335

Depositor

Marc Timmers

Article

Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

Insert

TAF4 (TAF4 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Functional C-terminal fragment 485-1085(end)

Promoter

LTR

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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pNUT N6His K206E hTFNG

Plasmid

#70130

Purpose

Expresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobe

Depositor

Ross MacGillivray, Anne Mason

Article

He et al Biochemistry. 1999 Jul 27;38(30):9704-11

Insert

mutated human serum transferrin (TF Human)

Use

Tags

N-terminal signal peptide, 4 aa link, 6 His, Fact…

Expression

Mammalian

Mutation

changed Lys206 to a glutamic acid, Asn413Asp, Asn…

Promoter

SV40

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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