We narrowed to 17,522 results for: emb

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pET SUMO-FL-syt7

Plasmid

#213630

Purpose

Bacterial expression of SUMO-tagged mouse FL-syt7

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

FL-syt7 (Syt7 Mouse)

Tags

SUMO

Expression

Bacterial

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pSF279

Plasmid

#167674

Purpose

Intermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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hMTP

Plasmid

#124281

Purpose

Expresses human MTP in mammalian cells.

Depositor

Mahmood Hussain

Article

Sellers et al J Biol Chem. 2003 May 30;278(22):20367-7

Insert

MTTP (MTTP Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pME-puro-SNAP-FLAG-CD59

Plasmid

#50374

Purpose

Express SNAP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

Depositor

Reika Watanabe

Article

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

SNAP

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pLPO2

Plasmid

#209976

Purpose

Contains Level 0 Part: replication origin (SH71rep) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

replication origin (SH71_rep)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLSU4cg10

Plasmid

#126078

Purpose

Plant transformation vector for stable expression of plastid targeted GFP1-10 in plant cells

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

Chloroplastic FNR transit peptide

Use

Binary

Tags

GFP1-10

Expression

Plant

Promoter

longCaMV35S

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pRU325

Plasmid

#167676

Purpose

Intermediate vector for eight gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mScarlet-EFS-Blast

Plasmid

#227260

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-3XMyc-mTrim25

Plasmid

#51394

Depositor

Narry Kim

Article

Kwon et al Nat Struct Mol Biol. 2013 Sep;20(9):1122

Insert

Trim25 (Trim25 Mouse)

Use

Lentiviral

Tags

3XMyc

Expression

Mammalian

Promoter

EF1alpha

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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Mut1-RS

Plasmid

#182881

Purpose

A pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut1-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.

Depositor

Miriam Amiram

Article

Hadar et al Chembiochem. 2021 Apr 16;22(8):1379-1384

Insert

Mut1-RS

Expression

Bacterial

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-eNOS-GFP

Plasmid

#22444

Depositor

William Sessa

Article

Sowa et al J Biol Chem. 1999 Aug 6. 274(32):22524-3

Insert

WT eNOS-GFP

Tags

GFP

Expression

Mammalian

Available Since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-VAMP5-IRES-Puromycin

Plasmid

#134662

Purpose

Expresses EGFP-tagged plasma membrane marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

vesicle-associated membrane protein 5 (VAMP5 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pMK382

Plasmid

#214373

Purpose

Tet-OsTIR1(F74G) (AAVS1 insertion donor)

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

Tet-OsTIR1(F74G)

Expression

Mammalian

Promoter

Tet ON 3G promoter

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-TRE3G-BCCP-PupE-IRES-BFP

Plasmid

#113405

Purpose

Express inducible BCCP-PupE in mammalian cell

Depositor

Min Zhuang

Article

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Insert

BCCP-PupE-IRES-BFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pMK445 (BSD-P2A-BromoTag)

Plasmid

#214375

Purpose

BromoTag for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

BSD-P2A-BromoTag

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pJRA108

Plasmid

#209341

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mCherry fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-YFP-raptor

Plasmid

#73385

Purpose

Expresses YFP and HA tagged Human raptor

Depositor

Jie Chen, Taekjip Ha

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

Regulatory Associated Protein Of MTOR, Complex 1 (RPTOR Human)

Tags

HA and YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
pLPC-NFLAG TRF1

Plasmid

#16058

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Mutation

aa2-439

Available Since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
Cag.Luc.puro

Plasmid

#74409

Purpose

Constitutive Luciferase expression in mammalian cells along with puromycin selection

Depositor

Georgios Stathopoulos

Article

Giannou et al EMBO Mol Med. 2017 May;9(5):672-686. doi

Insert

Luciferase

Expression

Mammalian

Promoter

CAG

Available Since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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pBIG2abc

Plasmid

#80617

Purpose

pBIG2abc - Step2 vector abc (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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