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pPOTv7-g418-mNG

Plasmid

#179821

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mNG

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-g418-mScarletI

Plasmid

#179822

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mScarlet-I

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-g418-mScarlet

Plasmid

#179823

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mScarlet

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv7-blast-SNAPtag

Plasmid

#179786

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SNAP

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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WT-short-UBASH3A-V5-6xHis

Plasmid

#192102

Purpose

Mammalian expression of human wild-type short-form UBASH3A with V5-6xHis tag

Depositor

Patrick Concannon

Publication

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Tags

V5, 6xHis

Expression

Mammalian

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0030

Plasmid

#172777

Purpose

Level 0 module containing NT2_nYFP

Depositor

Benjamin Field

Publication

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS N-terminal eYFP (1-174)

Use

Synthetic Biology

Mutation

no

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0031

Plasmid

#172778

Purpose

Level 0 module containing CT_nYFP

Depositor

Benjamin Field

Publication

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS N-terminal eYFP (1-174)

Use

Synthetic Biology

Mutation

no

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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SAE2-mGarnet

Plasmid

#175240

Purpose

Bacterial expression and purification, combined with SAE1 to form the E1 enzyme heterodimer

Depositor

Michael Rosen

Publication

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

UBA2 (UBA2 Human)

Expression

Bacterial

Promoter

tac

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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FRB-polySH35

Plasmid

#175234

Purpose

Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

Depositor

Michael Rosen

Publication

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

NCK1 (NCK1 Human)

Expression

Bacterial

Promoter

tac

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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DYNC2LI1

Plasmid

#132527

Purpose

Human LIC3, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Publication

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNC2LI1 (DYNC2LI1 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pET15b-GB

Plasmid

#129689

Purpose

express E. coli GreB

Depositor

Jeff Gelles

Publication

Tetone et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Insert

his6/thrombin/GreB (greB E. coli)

Tags

His6

Expression

Bacterial

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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dFP

Plasmid

#120542

Purpose

Expresses dFP biliverdin-binding fluorescence-activating full length protein in bacteria.

Depositor

Brian Chow

Publication

Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/

Insert

de novo fluorescence-activating protein (dFP)

Tags

6xHis - TEV cleavage

Expression

Bacterial

Promoter

T7

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3_K214R_Keima

Plasmid

#127143

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3_Keima (RPS3 Human)

Use

Lentiviral

Mutation

K214R

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3_K230R

Plasmid

#127134

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3 (RPS3 Human)

Use

Lentiviral

Mutation

K230R

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3_K227R

Plasmid

#127133

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3 (RPS3 Human)

Use

Lentiviral

Mutation

K227R

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_TNK1-1/1-SV1/3

Plasmid

#91548

Purpose

Protein expression and purification of human SH3 domain construct TNK1-1/1-SV1/3

Depositor

Sachdev Sidhu

Publication

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

TNK1-1/1-SV1/3 (TNK1 Human)

Expression

Bacterial

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGAP4-1/1

Plasmid

#91518

Purpose

Protein expression and purification of human SH3 domain construct ARHGAP4-1/1

Depositor

Sachdev Sidhu

Publication

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGAP4-1/1 (ARHGAP4 Human)

Expression

Bacterial

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3GL1-1/1

Plasmid

#91473

Purpose

Protein expression and purification of human SH3 domain construct SH3GL1-1/1

Depositor

Sachdev Sidhu

Publication

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3GL1-1/1 (SH3GL1 Human)

Expression

Bacterial

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SLA2-1/1

Plasmid

#91450

Purpose

Protein expression and purification of human SH3 domain construct SLA2-1/1

Depositor

Sachdev Sidhu

Publication

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SLA2-1/1 (SLA2 Human)

Expression

Bacterial

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGAP9-1/1

Plasmid

#91442

Purpose

Protein expression and purification of human SH3 domain construct ARHGAP9-1/1

Depositor

Sachdev Sidhu

Publication

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGAP9-1/1 (ARHGAP9 Human)

Expression

Bacterial

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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