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Structure of the dynein-2 complex and its assembly with intraflagellar transport trains.
Toropova K, Zalyte R, Mukhopadhyay AG, Mladenov M, Carter AP, Roberts AJ
Nat Struct Mol Biol. 2019 Aug 26. pii: 10.1038/s41594-019-0286-y. doi: 10.1038/s41594-019-0286-y.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
132524His-ZZ-TEV-SNAPf-DYNC2H1Full length human dynein 2 heavy chain DYNC2H1, Sf9 codon optimised. With 8xHis-ZZ tag, TEV cleavage site, SNAPf tag, precission cleavage site at N-terminus. Assembled from synthesised fragments
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132525WDR60Human WDR60, codon optimised for Sf9 expression.
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132526WDR34Human WDR34, codon optimised for Sf9 expression.
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132527DYNC2LI1Human LIC3, codon optimised for Sf9 expression.
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132528DYNLT1Human TCTEX-1, codon optimised for Sf9 expression.
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132529TCTEX1D2Human TCTEC-1D2, codon optimised for Sf9 expression.
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132530DYNLL1Human LC8-1, codon optimised for Sf9 expression.
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132531DYNLL2Human LC8-2, codon optimised for Sf9 expression.
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132532DYNLT3Human TCTEX-3, codon optimised for Sf9 expression.
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132533DYNLRB1Human Rbl-1, codon optimised for Sf9 expression.
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132534DYNLRB2Human Rbl-2, codon optimised for Sf9 expression.
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132536Dynein-2_complexFull length human (Hs) IFT dynein (dynein-2) complex for Sf9 expression. Contains His-ZZ-TEV-SNAPf-HC, WDR60, WDR34-Strep, LIC3, TCTEX-1, TCTEX1D2, LC8-1, LC8-2, TCTEX-3, Rbl-1, Rbl-2, LC8-like.
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133115LC8-likeHuman LC8-like protein, codon optimised for Sf9 expression.
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Recombinant Antibodies from Article