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Dynein-2_complex
(Plasmid #132536)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 132536 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pACEBac1
  • Backbone manufacturer
    Geneva Biotech
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 31586
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    DYNC2H1
  • Alt name
    Dynein 2 Heavy chain 1
  • Alt name
    DHC2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    12921
  • Mutation
    Codon optimised for Sf9 expression
  • Entrez Gene
    DYNC2H1 (a.k.a. ATD3, DHC1b, DHC2, DNCH2, DYH1B, SRPS2B, SRTD3, hdhc11)
  • Tags / Fusion Proteins
    • 8x His (N terminal on insert)
    • ZZ (N terminal on insert)
    • Linker-TEV-linker-TEV (N terminal on insert)
    • SNAPf (N terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    DYNC2LI1
  • Alt name
    LIC3
  • Alt name
    Cytoplasmic Dynein 2 Light Intermediate Chain 1
  • Alt name
    D2LIC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1056
  • Mutation
    Codon optimised for Sf9 expression
  • Entrez Gene
    DYNC2LI1 (a.k.a. CGI-60, D2LIC, LIC3)

Gene/Insert 3

  • Gene/Insert name
    WDR34
  • Alt name
    WD repeat-containing protein 34
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1695
  • Mutation
    Codon optimised for Sf9 expression
  • Entrez Gene
    WDR34 (a.k.a. DIC5, FAP133, SRTD11, bA216B9.3)
  • Tag / Fusion Protein
    • Strep (C terminal on insert)

Gene/Insert 4

  • Gene/Insert name
    WDR60
  • Alt name
    WD repeat-containing protein 60
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3201
  • Mutation
    Codon optimised for Sf9 expression
  • Entrez Gene
    WDR60 (a.k.a. FAP163, SRPS6, SRTD8)

Gene/Insert 5

  • Gene/Insert name
    DYNLL1
  • Alt name
    LC8-1
  • Alt name
    Dynein Light Chain LC8-Type 1
  • Alt name
    HDLC1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    270
  • Mutation
    Codon optimised for Sf9 expression
  • Entrez Gene
    DYNLL1 (a.k.a. DLC1, DLC8, DNCL1, DNCLC1, LC8, LC8a, PIN, hdlc1)

Gene/Insert 6

  • Gene/Insert name
    DYNLL2
  • Alt name
    LC8-2
  • Alt name
    Dynein Light Chain LC8-Type 2
  • Alt name
    DLC2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    270
  • Mutation
    Codon optimised for Sf9 expression
  • Entrez Gene
    DYNLL2 (a.k.a. DNCL1B, Dlc2, RSPH22)

Gene/Insert 7

  • Gene/Insert name
    DYNLRB1
  • Alt name
    Rbl-1
  • Alt name
    Roadblock-1
  • Alt name
    RB-1
  • Insert Size (bp)
    291
  • Mutation
    Codon optimised for Sf9 expression
  • Entrez Gene
    DYNLRB1 (a.k.a. BITH, BLP, DNCL2A, DNLC2A, ROBLD1)

Gene/Insert 8

  • Gene/Insert name
    DYNLRB2
  • Alt name
    Roadblock-2
  • Alt name
    Rbl-2
  • Alt name
    RB-2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    291
  • Mutation
    Codon optimised for Sf9 expression
  • Entrez Gene
    DYNLRB2 (a.k.a. DNCL2B, DNLC2B, ROBLD2)

Gene/Insert 9

  • Gene/Insert name
    DYNLT1
  • Alt name
    TCTEX-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    342
  • Mutation
    Codon optimised for Sf9 expression
  • Entrez Gene
    DYNLT1 (a.k.a. CW-1, TCTEL1, tctex-1)

Gene/Insert 10

  • Gene/Insert name
    TCTEX1D2
  • Alt name
    Tctex1 domain-containing protein 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    429
  • Mutation
    Codon optimised for Sf9 expression
  • Entrez Gene
    TCTEX1D2 (a.k.a. SRTD17)

Gene/Insert 11

  • Gene/Insert name
    DYNLT3
  • Alt name
    TCTEX-3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    351
  • Mutation
    Codon optimised for Sf9 expression
  • Entrez Gene
    DYNLT3 (a.k.a. RP3, TCTE1L, TCTEX1L)

Gene/Insert 12

  • Gene/Insert name
    LC8-like
  • Alt name
    LOC392067
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    894
  • Mutation
    Codon optimised for Sf9 expression
  • Entrez Gene
    LOC392067

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Cloned from genes synthesised by Epoch, codon optised for insect cell expression.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Cloned by Ruta Zalyte in Andrew Carter's lab. For cloning strategy, please refer to the Methods section of Toropova et al 2019.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Dynein-2_complex was a gift from Andrew Carter (Addgene plasmid # 132536 ; http://n2t.net/addgene:132536 ; RRID:Addgene_132536)
  • For your References section:

    Structure of the dynein-2 complex and its assembly with intraflagellar transport trains. Toropova K, Zalyte R, Mukhopadhyay AG, Mladenov M, Carter AP, Roberts AJ. Nat Struct Mol Biol. 2019 Aug 26. pii: 10.1038/s41594-019-0286-y. doi: 10.1038/s41594-019-0286-y. 10.1038/s41594-019-0286-y PubMed 31451806