Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

His-ZZ-TEV-SNAPf-DYNC2H1
(Plasmid #132524)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 132524 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pACEBac1
  • Backbone manufacturer
    Geneva Biotech
  • Backbone size w/o insert (bp) 2932
  • Total vector size (bp) 17001
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamycin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DYNC2H1
  • Alt name
    Dynein 2 Heavy chain 1
  • Alt name
    DHC2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    14069
  • Mutation
    Codon optimised for Sf9 expression
  • Entrez Gene
    DYNC2H1 (a.k.a. ATD3, DHC1b, DHC2, DNCH2, DYH1B, SRPS2B, SRTD3, hdhc11)
  • Tags / Fusion Proteins
    • 8x His (N terminal on insert)
    • ZZ (N terminal on insert)
    • Linker-TEV-linker-TEV (N terminal on insert)
    • Precission cleavage site (N terminal on insert)
    • SNAPf (N terminal on insert)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Cloned from genes synthesised by Epoch, codon optised for insect cell expression.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloned by Ruta Zalyte in Andrew Carter's lab. For cloning strategy, please refer to the Methods section of Toropova et al 2019.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His-ZZ-TEV-SNAPf-DYNC2H1 was a gift from Andrew Carter (Addgene plasmid # 132524 ; http://n2t.net/addgene:132524 ; RRID:Addgene_132524)
  • For your References section:

    Structure of the dynein-2 complex and its assembly with intraflagellar transport trains. Toropova K, Zalyte R, Mukhopadhyay AG, Mladenov M, Carter AP, Roberts AJ. Nat Struct Mol Biol. 2019 Aug 26. pii: 10.1038/s41594-019-0286-y. doi: 10.1038/s41594-019-0286-y. 10.1038/s41594-019-0286-y PubMed 31451806