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Showing: 3831 - 3840 of 9568 results

  1. pCR8/GW_TOPO_Cdk12_Nterm_FlagHa

    Plasmid
    #127177
    Purpose
    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector
    Depositor
    Phil Sharp
    Article
    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:
    Insert
    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    Use
    Entry vector with transgene for gateway cloning
    Tags
    Flag- HA- Tandem Epitopes
    Promoter
    None
    Available Since
    July 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    127177-plasmid-map-sequence-id-246566

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  2. pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)

    Plasmid
    #133794
    Purpose
    U6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    NmeCas9 and Nme2Cas9 sgRNA entry vector
    Expression
    Mammalian
    Mutation
    sgRNA architecture from Amrani et al. Genome Biol…
    Promoter
    U6
    Available Since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133794-plasmid-map-sequence-id-268076

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  3. pGEX-GST-BcPC-PLC

    Plasmid
    #224255
    Purpose
    Express N-terminal HRV 3C protease cleavable GST fused to Bacillus cereus PC-PLC in E.coli
    Depositor
    Chiaki Murakami
    Article
    Murakami et al Anal Biochem. 2017 Jun 1;526:43-49. doi:
    Insert
    Bacillus cereus phospholipase C (plC, plC Bacillus cereus ATCC 14579)
    Tags
    GST and HRV-3C (LEVLFQGP) site
    Expression
    Bacterial
    Mutation
    The gene of Bacillus cereus ATCC 14579 was used f…
    Promoter
    lac promoter
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    224255-plasmid-map-sequence-id-457146

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  8. pCAP03-sp44/p21

    Plasmid
    #120232
    Purpose
    Carries bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites that can be amplified with homology sequence for refactoring.
    Depositor
    Bradley Moore
    Article
    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-
    Insert
    bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites
    Use
    Synthetic Biology
    Mutation
    aac(3)IV (apramycin resistance gene)
    Available Since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    120232-plasmid-map-sequence-id-233750

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Showing: 3831 - 3840 of 9568 results