We narrowed to 60,203 results for: cloning

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pDT-sgRNA-XMAS-2x

Plasmid

#164414

Purpose

Dual targeted guide and cloning vector. Targets pEF-XMAS-2xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

Depositor

Xiao Wang

Article

Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

Insert

sgRNA

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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bdSUMO-R9-GFP-HiBit

Plasmid

#170990

Purpose

For bacterial expression of bdSUMO tagged muGFP with R9 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-R9-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pZA1

Plasmid

#158479

Purpose

Entry clone for Golden Gate assembly. Golden Gate compatible wild-type A. thaliana ZAR1 with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

STOP codon

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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Sha2Cas9

Plasmid

#192140

Purpose

Expresses Sha2Cas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

Insert

Sha2Cas9

Use

AAV

Expression

Mammalian

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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SpeCas9

Plasmid

#192139

Purpose

Expresses SpeCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

Insert

SpeCas9

Use

AAV

Expression

Mammalian

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pFOSGE_DUM1H01

Plasmid

#71090

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

nej (nej Fly)

Use

Gateway entry vector

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only
pQTEV-SARA1

Plasmid

#34809

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

SARA1 (SAR1A Human)

Tags

His

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 DNAJB5

Plasmid

#19527

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DnaJ (Hsp40) homolog, subfamily B, member 5 (DNAJB5 Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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BB3aN_14*

Plasmid

#98548

Purpose

empty BB3 for insertion and overexpression of 1 gene in P. pastoris

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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E2_pENTR_R4R3_T CTP

Plasmid

#171736

Purpose

ptpTALECD vector assembly, Entry clone 2 with attR4 and R3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

A terminator of Arabidopsis heat shock protein, RPS5A promoter, and RecA1 plastid targeting signal

Use

Multisite gateway entry vector

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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KTK_001

Plasmid

#180517

Purpose

Empty backbone for creation of entry parts for the KTK, contains sfGFP dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-zDicer

Plasmid

#171539

Purpose

Encodes Zebrafish Dicer protein

Depositor

Yan Zeng

Article

Li et al Biochem Biophys Res Commun. 2019 Jun 18;

Insert

dicer1(MK642699) (dicer1 Zebrafish)

Expression

Mammalian

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pC0.062

Plasmid

#119651

Purpose

Level 0 part. Terminator for luxICDABEG operon from Vibrio fischeri.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

Bba_B0011

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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BB1_34_RPS25Att

Plasmid

#98526

Purpose

BB1 with insert: RPS25Att

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

RPS25Att

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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KTK_247

Plasmid

#180529

Purpose

Entry vector containing pTac promoter

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

pTac promoter

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS DNAJA4

Plasmid

#19548

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DnaJ (Hsp40) homolog, subfamily A, member 4 (DNAJA4 Human)

Tags

His

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pCA0.001

Plasmid

#119557

Purpose

Level 0 acceptor vector for promoters truncated to transcriptional start site with lacZ compatible with blue/white screening

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA6/ERGIC2-WT

Plasmid

#51479

Purpose

Expresses ERGIC2-WT in mammalian cells

Depositor

Simon Kwok

Article

Kwok et al DNA Cell Biol. 2001 Jun;20(6):349-57.

Insert

ERGIC2-WT (ERGIC2 Human)

Tags

none

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

March 6, 2014

Availability

Academic Institutions and Nonprofits only
kank2_pJet1.2

Plasmid

#121980

Purpose

for generating kank2 riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank2 (LOC571549 Zebrafish)

Use

Cloning vector

Promoter

T7

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-2TAG

Plasmid

#158138

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the first serine residue…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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