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C234-E26: attB2r-Halotag-attB3

Plasmid

#162915

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal Halotag fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Halotag

Use

Synthetic Biology

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E14: attB4-EF1p>-attB5

Plasmid

#162975

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the human EF1alpha promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human EF1alpha promoter

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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512 pME-miR

Plasmid

#26032

Depositor

Nathan Lawson

Article

Nicoli et al Nature. 2010 Apr 22. 464(7292):1196-200.

Insert

ef1a (eef1a1l1 Zebrafish)

Use

Entry vector

Available Since

Aug. 26, 2010

Availability

Academic Institutions and Nonprofits only
mEmerald-RatLC3B-C-7

Plasmid

#54147

Purpose

Localization: Microtubule-Binding Protein, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Fong et al Autophagy. 2012 May 1;8(5):794-811. doi:

Insert

RatLC3B

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
C511-E02: attB5r-NanoLuc-nostop-attB1r

Plasmid

#162938

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal Nanoluciferase fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Nanoluciferase

Use

Synthetic Biology

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E33: attB4-UbCp>-attB1r

Plasmid

#162925

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human Ubiquitin C promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human Ubiquitin C promoter

Use

Synthetic Biology

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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C232-E29: attB2r-mClover3-attB3

Plasmid

#162906

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal mClover3 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Clover3 fluorescent protein

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C232-E30: attB2r-mRuby3-attB3

Plasmid

#162907

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal mRuby3 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Ruby3 fluorescent protein

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C234-E25: attB2r-SNAP-attB3

Plasmid

#162914

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal SNAP tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

SNAP tag

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E02: attB4-CAGp>-attB5

Plasmid

#162972

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the CAG promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

chicken beta-actin/CMV enhancer promoter

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E24: attB4-UbCp>-attB5

Plasmid

#162980

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the human Ubiquitin C promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human Ubiquitin C promoter

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E23: attB5r-mRuby3-nostop-attB1r

Plasmid

#162945

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mRuby3 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Ruby3 fluorescent protein

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E22: attB5r-mClover3-nostop-attB1r

Plasmid

#162944

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mClover3 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Clover3 fluorescent protein

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C238-E02: attB2r-BGHpolyA-attB3

Plasmid

#162916

Purpose

Gateway attB2r/attB3 entry clone containing a polyA sequence for transcription termination

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Bovine growth hormone polyadenylation sequence

Use

Synthetic Biology

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TRPgamma

Plasmid

#83271

Purpose

Mammalian expression plasmid for Drosophila TRPgamma cDNA

Depositor

Craig Montell

Article

Xu et al Neuron. 2000 Jun;26(3):647-57.

Insert

TRPgamma (Trpgamma Fly)

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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C453-E23: attB4-TRE3Gp>-attB5

Plasmid

#162979

Purpose

Gateway attB4/attB5 entry clone for inducible mammalian expression using the TRE3G promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Tet-regulated TRE3G minimal promoter

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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776 pME-miR-126

Plasmid

#26033

Depositor

Nathan Lawson

Article

Nicoli et al Nature. 2010 Apr 22. 464(7292):1196-200.

Insert

miR-126 (mir126a Zebrafish)

Use

Entry vector

Available Since

Aug. 26, 2010

Availability

Academic Institutions and Nonprofits only
pRK5 Flag-RagA(R37P)

Plasmid

#99717

Purpose

Mammalian expression of Flag-RagA(R37P)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagA (RRAGA )

Tags

Flag

Mutation

R37P

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-RagC(K84T)

Plasmid

#99722

Purpose

Mammalian expression of HA-RagC(K84T)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagC (RRAGC )

Tags

HA

Mutation

K84T

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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C512-E04: attB5r-mCherry-nostop-attB1r

Plasmid

#162941

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mCherry fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Cherry fluorescent protein

Use

Synthetic Biology

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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