Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 62,918 results for: cloning

Clear keywords
Showing: 7861 - 7880 of 62918 results

  1. JI501: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 gRNA scaffold

    Plasmid
    #121841
    Purpose
    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven SaCas9 gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to dCas9 w/ gRNA expression
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Gateway Cloning and Synthetic Biology
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121841-plasmid-map-sequence-id-237395

    Enlarge

  2. LF701: pMAGIC (L1-R5) hU6::xCas9(3.7) gRNA scaffold

    Plasmid
    #121817
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Gateway Cloning and Synthetic Biology
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121817-plasmid-map-sequence-id-237365

    Enlarge

  3. FB007

    Plasmid
    #119705
    Purpose
    Promoter gpdA from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.
    Depositor
    Jose Marcos
    Publication
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Promoter gpdA
    Use
    Synthetic Biology; Domestication of dna parts for…
    Promoter
    gpdA
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119705-plasmid-map-sequence-id-237147

    Enlarge

  6. pLV-H1-SGIBX

    Plasmid
    #174843
    Purpose
    empty vector for cloning shRNA and/or miRNA
    Depositor
    Helen Morrison
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Available since
    Nov. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    174843-plasmid-map-sequence-id-347986

    Enlarge

  7. pLV-H1-SX

    Plasmid
    #174845
    Purpose
    empty vector for cloning shRNA and/or miRNA
    Depositor
    Helen Morrison
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Available since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174845-plasmid-map-sequence-id-344688

    Enlarge

  8. pDNR-hAkt1

    Plasmid
    #49387
    Purpose
    donor vector for cloning human Akt1 with 6xHN tag
    Depositor
    Randall McKinnon
    Insert
    Akt1 (AKT1 Human)
    Use
    Cre/Lox; Donor vector
    Tags
    6xHN (6 repeating His-Asn subunits)
    Mutation
    tga to cca at nt 1725 with respect to GenBank ID …
    Promoter
    none
    Available since
    Dec. 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. pGGSG-Bromo-T7-P2a-Neo

    Plasmid
    #235516
    Purpose
    Cloning vector for GGSG-BromoTag-T7-P2A-Neo. Clone into ZraI and AfeI
    Depositor
    Brian Hendrich
    Insert
    BromoTag
    Use
    Mouse Targeting
    Tags
    BromoTag, T7, neo
    Mutation
    None
    Available since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    235516-plasmid-map-sequence-id-474044

    Enlarge

  10. pGGSG-Bromo-T7-T2a-Pac

    Plasmid
    #235518
    Purpose
    Cloning vector for GGSG-BromoTag-T7-T2A-Pac. Clone into PmlI and AfeI
    Depositor
    Brian Hendrich
    Insert
    BromoTag
    Use
    Mouse Targeting
    Tags
    BromoTag, T7, Puro
    Mutation
    None
    Available since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    235518-plasmid-map-sequence-id-474042

    Enlarge

  11. pGGSG-FKBP-3xF-T2a-Bsd

    Plasmid
    #235520
    Purpose
    Cloning vector for GGSG-FKBP-3xFLAG-T2A-Bsd. Clone into ZraI and AfeI
    Depositor
    Brian Hendrich
    Insert
    FKBP
    Use
    Mouse Targeting
    Tags
    Fkbp, 3xFALG, Bsd
    Mutation
    None
    Available since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    235520-plasmid-map-sequence-id-474011

    Enlarge

  12. pGGSG-Bromo-T7-T2a-Bsd

    Plasmid
    #235519
    Purpose
    Cloning vector for GGSG-BromoTag-T7-T2A-Bsd. Clone into ZraI and AfeI
    Depositor
    Brian Hendrich
    Insert
    BromoTag
    Use
    Mouse Targeting
    Tags
    BromoTag, T7, Bsd
    Mutation
    None
    Available since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    235519-plasmid-map-sequence-id-474008

    Enlarge

  13. pGGSG-Bromo-T7-P2a-Pac

    Plasmid
    #235517
    Purpose
    Cloning vector for GGSG-BromoTag-T7-P2A-Pac. Clone into PmlI and AfeI
    Depositor
    Brian Hendrich
    Insert
    BromoTag
    Use
    Mouse Targeting
    Tags
    BromoTag, T7, Puro
    Mutation
    None
    Available since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    235517-plasmid-map-sequence-id-473996

    Enlarge

  14. PB-TRE3G-CCRB

    Plasmid
    #236772
    Purpose
    A piggybac-based cloning vector containing TRE3G promoter followed by cloning site and CAG promoter-driven nuclear-localized Clover-P2A-rtTA-IRES-BSD.
    Depositor
    Kazutoshi Takahashi
    Publication
    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do
    Type
    Empty backbone
    Use
    Piggybac
    Expression
    Mammalian
    Promoter
    TRE3G promoter, CAG promoter
    Available since
    May 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    236772-plasmid-map-sequence-id-473454

    Enlarge

Showing: 7861 - 7880 of 62918 results