We narrowed to 16,131 results for: form

1. pAAV-CAG-DIO-ChroME2f-ST-P2A-H2B-mRuby3

   Plasmid

   #171150

   Purpose

   Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

   Depositor

   Hillel Adesnik

   Article

   Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

   Insert

   ChroME2f-P2A-H2B-mRuby3
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV_SEPT9_i1-msfGFP

   Plasmid

   #180328

   Purpose

   mammalian expression of human SEPT9_i1 fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

   Insert

   SEPTIN9_v1 (SEPTIN9 Human)
   

   Tags

   msfGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSYN-DRD2-V2tail-TevN-BLITz1-TetR-VP16-bGHpA

   Plasmid

   #89874

   Purpose

   expresses DRD2-iTango component in mammalian cells for AAV production

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   DRD2-V2tail-TevN-BLITz1-TetR-VP16
   

   Use

   AAV

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   short isoform of DRD2 (deletion of 242-270)

   Promoter

   Human synapsin

   Available Since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRX-IP/SECFP-hATG16A1

   Plasmid

   #58994

   Purpose

   Expresses SECFP-hATG16A1 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

   Insert

   Homo sapiens autophagy related 16-like 1 (S. cerevisiae) (ATG16L1 Human)
   

   Use

   Retroviral

   Tags

   SECFP

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C1 hSlp4-a

   Plasmid

   #40032

   Depositor

   Fernando Martin-Belmonte

   Article

   Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

   Insert

   hSlp4-a (SYTL4 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Oct. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pBW2426_pCAG-PV1-iCre-N270-L1-GID1-NLS-BGHpA

   Plasmid

   #108729

   Purpose

   Expresses N-terminus to aa270 of Cre recombinase fused to GID1 CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Cre recombinase fragment N-terminus to aa270
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GID1,NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. actc1b-GcAMP5

   Plasmid

   #126563

   Purpose

   Muscle specific expression of the calcium reporter GcAMP5 in zebrafish. Parton lab clone APT

   Depositor

   Rob Parton

   Article

   Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

   Insert

   GcAMP5
   

   Available Since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 barr1 flag

   Plasmid

   #14687

   Depositor

   Robert Lefkowitz

   Article

   Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

   Insert

   beta arrestin 1 (Arrb1 Rat)
   

   Tags

   flag

   Expression

   Mammalian

   Available Since

   June 19, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pFUW-TetO-Junb

   Plasmid

   #178441

   Purpose

   doxycycline-inducible expression of Mouse Junb in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

   Insert

   Junb (Junb Mouse)
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOPINB-hRNF216

    Plasmid

    #167354

    Purpose

    Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells

    Depositor

    Bernhard Lechtenberg

    Article

    Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

    Insert

    E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
    

    Tags

    3C protease cleavage site and 6x-His tag

    Expression

    Bacterial

    Mutation

    codon optimised for expression in E. coli

    Promoter

    T7

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLCHKO_HPRT1_exon2_1_Lb

    Plasmid

    #155051

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBW2209_pCAG-PV1-iCre-N229-L1-GID1-NLS-BGHpA

    Plasmid

    #108723

    Purpose

    Expresses N-terminus to aa229 of Cre recombinase fused to GID1 CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment N-terminus to aa229
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GID1,NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBW2229_pCAG-PV1-GAI-L1-iCre-230C-NLS-BGHpA

    Plasmid

    #108724

    Purpose

    Expresses aa230 to C-terminus of Cre recombinase fused to GAI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment aa230 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GAI and NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19_CoV-2_linker

    Plasmid

    #211731

    Purpose

    Linker fragment for direct rescue of SARS-CoV-2, including 5' Promoter and 3' Termination signals and 100bp SARS-CoV-2 homology regions

    Depositor

    Thomas Klimkait

    Article

    Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

    Insert

    Linker fragment SARS-CoV-2
    

    Expression

    Mammalian

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL4.23_Promoter_1-3

    Plasmid

    #210629

    Purpose

    Luciferase experiment for TAL1 promoter 1-3

    Depositor

    Maayan Salton

    Article

    Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

    Insert

    promoter 1-3 (TAL1 Human)
    

    Expression

    Mammalian

    Promoter

    Minimal Promoter

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-Rab8a-WT

    Plasmid

    #101048

    Purpose

    Expresses HA-tagged human Rab8-WT in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Article

    Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

    Insert

    Rab8a (RAB8A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETDuet1-MBP-NAAEF-PNR(217-410 C275)-His6

    Plasmid

    #177849

    Purpose

    Bacterial Expression of PNR

    Depositor

    Karsten Melcher

    Article

    Tan et al PLoS One. 2013 Sep 12;8(9):e74359. doi:

    Insert

    photoreceptor cell-specific nuclear receptor (NR2E3 Human)
    

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLCHKO_HPRT1_exon3_1_Lb

    Plasmid

    #155053

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 3
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. PD2529 human alpha5 full

    Plasmid

    #221395

    Purpose

    Mammalian expression of human integrin alpha5 full length

    Depositor

    Timothy Springer

    Article

    Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

    Insert

    integrin alpha5 full length (ITGA5 Human)
    

    Tags

    CD33 secretion peptide (MPLLLLLPLLWAGALA)

    Expression

    Mammalian

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-IRES-tdTomato-FlagAkt1W80A

    Plasmid

    #64833

    Purpose

    cDNA of Akt1 with point mutation from Trp to Ala at position 80. This mutation confers resistance to the MK-2206 drug

    Depositor

    Eva Gonzalez

    Article

    Kajno et al Biochem J. 2015 Apr 9.

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Point mutation from Trp to Ala at position 80

    Promoter

    CMV

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only