We narrowed to 15,953 results for: grna

1. pYPQ141D-peg

   Plasmid

   #141081

   Purpose

   Gateway compatible prime editing gRNA empty backbone vector

   Depositor

   Yiping Qi

   Article

   Tang et al Mol Plant. 2020 Mar 25. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX459-APP-KO

   Plasmid

   #176487

   Purpose

   To knockout APP gene

   Depositor

   Wade Harper

   Article

   Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

   Insert

   gRNA sequence
   

   Use

   CRISPR

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEasyCas9

   Plasmid

   #184909

   Purpose

   Expresses the Streptococcus pyogenes Cas9 protein

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRDA_887

   Plasmid

   #201164

   Purpose

   Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65 and NLS

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Promoter

   EFS

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDIRECT_23A

   Plasmid

   #91138

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:bar

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:AtCas9 + AtU6:gRNA
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMOD_B2520

   Plasmid

   #91077

   Purpose

   Module B, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning
   

   Use

   CRISPR

   Promoter

   OsU6

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Sg-A

   Plasmid

   #83807

   Purpose

   Express sgRNA targeting SA site

   Depositor

   Bo Feng

   Article

   He et al Nucleic Acids Res. 2016 May 19;44(9):e85

   Insert

   SgRNA
   

   Use

   CRISPR

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. SiC-V1-Scr

   Plasmid

   #133042

   Purpose

   SiC-V1 vector with scramble (Scr) sgRNA. This lentivirus construct is used for stably expressing spCas9 with a dTomato reporter in a cell line of interest.

   Depositor

   Sriram Neelamegham

   Article

   Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

   Insert

   SpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   dTomato

   Available Since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pST_119_LVL2 cam

   Plasmid

   #179333

   Purpose

   NT-CRISPR plasmid for integration of multiple gRNAs.

   Depositor

   Anke Becker

   Article

   Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

   Insert

   Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, sfGFP dropout to be replaced with gRNAs
   

   Use

   CRISPR

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDIRECT_10A

    Plasmid

    #91207

    Purpose

    Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: none

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    35S:AtCas9 + AtU6:gRNA
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ132A2.0

    Plasmid

    #99887

    Purpose

    Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU6

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ133A2.0

    Plasmid

    #99891

    Purpose

    Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU6

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330-PITCh-H2BC11

    Plasmid

    #207755

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H2BC11 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of H2BC11 (H2BC11 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ131B2.0

    Plasmid

    #99885

    Purpose

    Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU3

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHDE-35S-Cas9-mCherry-UBQ

    Plasmid

    #78932

    Purpose

    For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants. Enable production of two gRNAs

    Depositor

    Yunde Zhao

    Article

    Gao et al Plant Physiol. 2016 May 15. pii: pp.0066

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUDP004

    Plasmid

    #101165

    Purpose

    E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    ScTDH3

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330-EN479

    Plasmid

    #86234

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locus

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    spCas9-nickase and sgRNA against mouse rosa26 locus
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBAtC

    Plasmid

    #78097

    Purpose

    Plant expression of Cas9 and gRNA empty backbone; Basta resistance

    Depositor

    Jin-Soo Kim

    Article

    Kim et al J Integr Plant Biol. 2016 Mar 4. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    NLS-HA

    Expression

    Plant

    Promoter

    U6

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAtC

    Plasmid

    #78098

    Purpose

    Plant expression of Cas9 and gRNA empty backbone; Hygro Resistance

    Depositor

    Sang-Gyu Kim, Jin-Soo Kim

    Article

    Kim et al J Integr Plant Biol. 2016 Mar 4. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    U6

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYPQ133B2.0

    Plasmid

    #99892

    Purpose

    Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU3

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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