We narrowed to 62,186 results for: cloning
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Plasmid#224482PurposeGateway compatible 5' entry clone containing the Zebrafish unc-503 promoter with a minimal c-fos promoter and b-globin intronDepositorInsertUnc-503 promoter
UseGateway CloningAvailable SinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME V5 mScarlet-I (JDW 1323)
Plasmid#224487PurposeGateway compatible middle entry clone containing V5 tagged m-Scarlet-I (Cytosolic far red fluorescent reporter)DepositorInsertV5-mScarlet-I
UseGateway CloningAvailable SinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-LexA-mODC-LexOP-35SCaMV (JDW 1348)
Plasmid#224505PurposeGateway compatible middle entry clone containing a Destablized LexA and LexOperon with minimal CaMV 35S promoter as an all in one casette for RU486 driven gene expressionDepositorInsertLexA-mODC-LexOP-35SCaMV
UseGateway CloningAvailable SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)
Plasmid#224512PurposeA Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)DepositorInsert3xHA-BirA-T2A-mCherry-Caax
UseGateway CloningAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME nls EGFP-Cre (JDW 1329)
Plasmid#224515PurposeA Gateway compatible middle entry clone containing a NLS-EGFP-Cre fusionDepositorInsertnls-EGFP-Cre
UseCre/LoxAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME iCre-I (JDW 1203)
Plasmid#224520PurposeA Gateway compatible middle entry clone containing a loxP flanked, self inactivating Cre that contains an intron to prevent recombination in bacteriaDepositorInsertiCre-I
UseCre/LoxAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox (JDW 1232)
Plasmid#224522PurposeA Gateway compatible middle entry clone containing loxP flanked 3xNLS-mCherry-V5-3xSTOP cassetteDepositorInsertlox-3xNLS-mCherry-V5-SV40pA-3xstop-lox
UseCre/LoxAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E EFS-rtTA/rtTA3 (JDW 1214)
Plasmid#224533PurposeA Gateway compatible 3' entry clone containing the Human EFS promoter driving rtTA-3rd generation transactivatorDepositorInsertrtTA
UseGateway CloningPromoterEFSAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-Actin-Vhh-mNeonGreen-HA (JDW 1225)
Plasmid#224534PurposeA Gateway compatible 3' entry clone containing an Actin nanobody fused to mNeonGreen with a c-terminal HA tagDepositorInsertActin-Vhh-mNeonGreen-HA
UseGateway CloningAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-DEST-Myc
Plasmid#119752PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Myc tag.DepositorTypeEmpty backboneTagsGST and MycExpressionBacterialPromotertac promoterAvailable SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TOgw-C-mEGFP
Plasmid#223953PurposeGateway cloning of genes of interest with a C-terminal mEGFP fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneTagsmEGFPExpressionMammalianAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TOgw-C-mECFP
Plasmid#223950PurposeGateway cloning of genes of interest with a C-terminal mECFP fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneTagsmECFPExpressionMammalianAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TOgw-C-mEYFP
Plasmid#223951PurposeGateway cloning of genes of interest with a C-terminal mEYFP fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneTagsmEYFPExpressionMammalianAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TOgw-N-mEGFP
Plasmid#223949PurposeGateway cloning of genes of interest with an N-terminal mEGFP fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneTagsmEGFPExpressionMammalianAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKP579
Plasmid#159598PurposeExpress Cba and Cbi proteinDepositorInsertcba and cbi
ExpressionBacterialMutationnoneAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R777-E112 Hs.IRS2-nostop
Plasmid#70396PurposeGateway ORF clone of codon optimized human IRS2 [NM_003749] without stop codon (for C-terminal fusions)DepositorInsertIRS2 (IRS2 Human)
UseGateway CloningAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRDB_345
Plasmid#231113PurposeABE activity-based selection cloning vectorDepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-MCS-Ds
Plasmid#102416PurposeEmpty vector with a multiple cloning site flanked by Ds integration elementsDepositorTypeEmpty backboneExpressionBacterialAvailable SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BbsI)sgRNA_CAG-Cas9-venus-bpA
Plasmid#86986PurposeFor cloning and expression of sgRNA together with expression of a Cas9-Venus fusion proteinDepositorInsertCas9-venus
TagsCas9-Venus fusion proteinExpressionMammalianPromoterCAGAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
GG-dest
Plasmid#69538PurposeDestination plasmid for gRNA concatenation Golden Gate cloningDepositorTypeEmpty backboneAvailable SinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits only