We narrowed to 10,134 results for: yeast

1. pBS-SKII-URA-GAL1-fSNAP-3xHA

   Plasmid

   #188935

   Purpose

   Integration cassette to create N-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   URA-GAL1-fSNAP-3xHA
   

   Expression

   Yeast

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTUP1

   Plasmid

   #104341

   Purpose

   Express TUP1

   Depositor

   Alexander Varshavsky

   Article

   Xia et al J Biol Chem. 2008 Oct 24;283(43):28958-6

   Insert

   TUP1 (TUP1 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS267

   Plasmid

   #122380

   Purpose

   CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codon

   Depositor

   Rebecca Shapiro

   Article

   Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

   Insert

   HSP90
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMJ123: pRS426-His-MBP-SpuFz1_PSP1

   Plasmid

   #205256

   Purpose

   S. cerevisiae expression vector for SpuFz1 reprogrammed guide (targeting PSP1)

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1
   

   Tags

   10xHis, MBP

   Expression

   Yeast

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. RIB VP64 R1

   Plasmid

   #161493

   Purpose

   CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R1_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (Targeting R1 in the RIB1 promoter)
   

   Expression

   Yeast

   Promoter

   pGAP, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYC56

   Plasmid

   #63910

   Purpose

   Recipient integrative vector to construct mCherry tagged fusions

   Depositor

   Irene Castano Navarro

   Article

   Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

   Type

   Empty backbone

   Tags

   mCherry

   Expression

   Yeast

   Available Since

   April 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWS2247

   Plasmid

   #123026

   Purpose

   Tet promoter (TetO(x7)-pLEU2m)

   Depositor

   Tom Ellis

   Article

   Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

   Insert

   pTet [2]
   

   Expression

   Yeast

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPpT4- PGK1prom-TIR1-FLAG

   Plasmid

   #189724

   Purpose

   Enables expression of OsTIR1 from the PGK1 promoter, used to implement the AID system in Komagataella phaffii (original name pAEA454)

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

   Insert

   OsTIR1
   

   Expression

   Yeast

   Promoter

   PGK1 promoter

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSSN6

   Plasmid

   #104340

   Purpose

   Express SSN6

   Depositor

   Alexander Varshavsky

   Article

   Xia et al J Biol Chem. 2008 Oct 24;283(43):28958-6

   Insert

   SSN6 (CYC8 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMJ240: pRS426-His-MBP-MmeFz2_Native

    Plasmid

    #205255

    Purpose

    S. cerevisiae expression vector for MmeFz2 native guide

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    MmeFz2
    

    Tags

    10xHis, MBP

    Expression

    Yeast

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Htb2-GFP

    Plasmid

    #117669

    Purpose

    Colorize the nuclei

    Depositor

    Pau Ferrer, Benjamin Glick

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    Htb2-GFP
    

    Expression

    Yeast

    Mutation

    Contains the gene encoding the Histone Htb2 from …

    Promoter

    pGAP

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRG714_lexO-AscI_LexA-TF_URA3MX

    Plasmid

    #154822

    Purpose

    Inducible expression of AscI endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    AscI endonuclease
    LexA-ER-B112
    

    Tags

    FLAG and NLS

    Expression

    Yeast

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRG645_lexO-AscI_LexA-TF_LEU2MX

    Plasmid

    #154813

    Purpose

    Inducible expression of AscI endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    AscI endonuclease
    LexA-ER-B112
    

    Tags

    FLAG and NLS

    Expression

    Yeast

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. p404-Grx5-GFP1-10

    Plasmid

    #91952

    Purpose

    Expresses GFP1-10 in the mitochondrial matrix

    Depositor

    Rong Li

    Article

    Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

    Insert

    Grx5-GFP1-10
    

    Expression

    Yeast

    Promoter

    GAP

    Available Since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. MAP72_MS2

    Plasmid

    #220623

    Purpose

    Cassette vector to enable overexpression of rNTases as MS2 coat protein fusions in S. cerevisiae.

    Depositor

    Marvin Wickens

    Article

    Preston et al Nat Methods. 2019 May;16(5):437-445. doi

    Type

    Empty backbone

    Use

    Shuttle vector

    Expression

    Yeast

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYES2-Ub-R-GFP

    Plasmid

    #11953

    Depositor

    Nico Dantuma

    Article

    Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

    Insert

    Ub-R-GFP
    

    Expression

    Yeast

    Mutation

    Ubiquitin fused to N-terminus of GFP. Arginine at…

    Available Since

    May 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTCW012

    Plasmid

    #83527

    Purpose

    Cloning destination vector for phermone mediated expression from the FUS1J2 promoter and CYC1 terminator

    Depositor

    Claudia Vickers

    Article

    Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    pFUS1J2

    Available Since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pADH137

    Plasmid

    #90986

    Purpose

    NAT-marked CAS9 expression construct; part 1 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH118-series gRNA expression constructs.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Inserts

    C. albicans LEU2 1 of 2
    pENO1
    Cas9
    NAT 1 of 2
    

    Use

    CRISPR

    Available Since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-Lifeact-Dronpa

    Plasmid

    #182330

    Purpose

    Actin-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    Lifeact-Dronpa
    

    Tags

    N-terminal 17 amino acids from budding yeast Abp1…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pADH140

    Plasmid

    #90988

    Purpose

    NAT-marked CAS9 expression construct; part 1 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH143-series gRNA expression constructs.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Inserts

    C. maltosa LEU2 1 of 2
    pENO1
    Cas9
    NAT 1 of 2
    

    Use

    CRISPR

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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