164,722 results

1. T7-pAc5.1 mCherry EcoRV

   Plasmid

   #206490

   Purpose

   Schneider cell imaging

   Depositor

   Anne Ephrussi

   Article

   Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

   Type

   Empty backbone

   Use

   Tags

   mCherry

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDONR223-HK2

   Plasmid

   #23854

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   HK2 (HK2 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pDest-521

   Plasmid

   #159688

   Purpose

   Gateway destination vector for E. coli protein expression

   Depositor

   Dominic Esposito

   Article

   Tran et al Nat Commun. 2021 Feb 19;12(1):1176. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRS305-Leu2-PINO4-PfV-GS-Sapphire

   Plasmid

   #116930

   Purpose

   Yeast integrative vector for the exression of the 40nm GEM under the INO4 promoter

   Depositor

   Liam Holt

   Article

   Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

   Insert

   Pfv
   

   Use

   Tags

   Sapphire

   Expression

   Yeast

   Mutation

   Promoter

   INO4

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-mCherry-CDK4KTR

   Plasmid

   #126680

   Purpose

   Fluorescent reporter for CDK4/6 activity

   Depositor

   Hee Won Yang

   Article

   Yang et al Elife. 2020 Apr 7;9. pii: 44571. doi: 10

   Insert

   RB cterm
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-sgBrca2#1/Cre

   Plasmid

   #173627

   Purpose

   Expresses a Brca2-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Brca2 (Brca2 Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AICSDP-10:LMNB1-mEGFP

   Plasmid

   #87422

   Purpose

   Homology arms and linker-mEGFP sequence for N-terminus tagging of human LMNB1

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   LMNB1 Homology Arms with mEGFP-linker (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available Since

   March 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pdCas9-sgRNA-RFP

   Plasmid

   #166005

   Purpose

   Bacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)

   Depositor

   Martin Buck

   Article

   Bradley et al Biotechnol Rep (Amst). 2021 Oct 9;32:e00

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ADCYAP1R1-Tango

   Plasmid

   #66208

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   ADCYAP1R1 (ADCYAP1R1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

10. CCR4-Tango

    Plasmid

    #66241

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    CCR4 (CCR4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-FLuc

    Plasmid

    #170575

    Purpose

    Express firefly luciferase. Used in MLV-based SARS-CoV-2 pseudovirus assay.

    Depositor

    David Nemazee

    Article

    Rogers et al Science. 2020 Aug 21;369(6506):956-963.

    Insert

    Luc
    

    Use

    Luciferase and Retroviral

    Tags

    NA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Active Wnt10B-V5

    Plasmid

    #43823

    Depositor

    Xi He

    Article

    MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

    Insert

    WNT10B (WNT10B Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pExp-Sumo

    Plasmid

    #112574

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Sumo fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8His and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HIP-flash (HOP-flash mutant)

    Plasmid

    #83466

    Purpose

    Negative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter gene

    Depositor

    Barry Gumbiner

    Article

    Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

    Insert

    Multimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 2Bc-T MBP_BoMoC(ed)_6xH

    Plasmid

    #186461

    Purpose

    Recombinant B. mori truncated R2 RT expression

    Depositor

    Kathleen Collins

    Article

    Upton et al Proc Natl Acad Sci U S A. 2021 Oct 19;11

    Insert

    B. mori truncated R2 RT
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPaint-mNeon-PuroR

    Plasmid

    #174090

    Purpose

    CRISPaint gene tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    mNeonGreen
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOCC65

    Plasmid

    #118865

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric GFP

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-mGFP-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease, mGFP

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223-PIK3R5

    Plasmid

    #23819

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PIK3R5 (PIK3R5 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pNuc-TrxRFP1

    Plasmid

    #98998

    Purpose

    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, nuclear localization

    Depositor

    Huiwang Ai

    Article

    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

    Insert

    TrxRFP1 and nuclear localization sequence
    

    Use

    Tags

    His-tag and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAB744

    Plasmid

    #176579

    Purpose

    Expressing the whole n-butanol pathway.

    Depositor

    Arpita Bose

    Article

    Bai et al Commun Biol. 2021 Nov 3;4(1):1257. doi:

    Insert

    phaJ, ter, adhE2, phaA, phaB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge