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pRGEN-CMV-10XGCN4-L1-dCjCas9 S-D

Plasmid

#169912

Purpose

Expression of a SunTag-dCjCas9 fusion protein in which 10 copies of the GCN4 peptide are fused to the N terminus of dCjCas9.

Depositor

Zhili Rong

Article

Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

Insert

dCjCas9

Use

CRISPR

Tags

10XGCN4, 2xSV40NLS, and SV40NLS-HA

Expression

Mammalian

Mutation

D8A, H559A in cjCas9

Promoter

CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBV-Luc wt MBS1-4

Plasmid

#16564

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

c-MYC binding sites (MYC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only

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pCSDest

Plasmid

#22423

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Type

Empty backbone

Available Since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_NUP98_HOXD13

Plasmid

#205871

Purpose

Express mEGFP-tagged fusion protein, NUP98_HOXD13 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

NUP98_HOXD13 (NUP98, HOXD13 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLX-sgRNA

Plasmid

#50662

Purpose

Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

Depositor

Eric Lander, David Sabatini

Article

Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

Insert

sgAAVS1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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hIRE1a wt

Plasmid

#20744

Depositor

Fumihiko Urano

Article

Lipson et al PLoS ONE. 2008 . 3(2):e1648.

Insert

IRE1 (ERN1 Human)

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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pGL403

Plasmid

#119953

Purpose

Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

Inserts

gpps
limS

Expression

Bacterial

Mutation

N-terminal truncation of 56 amino acid residues (…

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pIB4

Plasmid

#25453

Depositor

Benjamin Glick

Article

Sears et al Yeast. 1998 Jun 15;14(8):783-90.

Insert

Pichia pastoris HIS4 (PAS_chr1-4_0160 Pichia pastoria)

Use

Pichia pastoris expression

Available Since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF)

Plasmid

#101139

Purpose

Luciferase reporter plasmid. Note that both renilla luciferase and ORF-firefly luciferase are encoded by a single mRNA and both are translated independently via internal ribosomal binding sites.

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-Calreticulin-N-16

Plasmid

#55006

Purpose

Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Calreticulin (CALR Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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Ac5-STABLE1-neo

Plasmid

#32425

Depositor

Rosa Barrio, James Sutherland

Article

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Insert

GFP-T2A-neo

Expression

Insect

Promoter

Actin5c

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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CROP-sgRNA-MS2

Plasmid

#153457

Purpose

CROP-seq vector with mCherry and x2 MS2 loops in gRNA scaffold

Depositor

Wolf Reik

Article

Alda-Catalinas et al Cell Syst. 2020 Jul 22;11(1):25-41.e9. d

Insert

sgRNA empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pKan-AID*-9myc

Plasmid

#99522

Purpose

C-terminal AID*-9myc degron cassette with KanR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pHSV-ELGA1

Plasmid

#246926

Purpose

Bioluminescent endolysosomal Ca2+ indicator lower Ca2+ affinity

Depositor

Teresa Alonso

Article

Calvo et al J Cell Biol. 2025 Dec 1;224(12):e2024100

Insert

ELGA1 (Endo-lysosome GFP-Aequorin)

Use

Herpes viral vector

Expression

Mammalian

Mutation

D117A, D119A, D163A in aequorin gene

Promoter

IE4/5 promoter (HSV)

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pHSV-ELGA

Plasmid

#246796

Purpose

Bioluminescent endolysosomal Ca2+ indicator

Depositor

Teresa Alonso

Article

Calvo et al J Cell Biol. 2025 Dec 1;224(12):e2024100

Insert

ELGA (Endo-lysosome GFP-Aequorin)

Use

Herpes viral vector

Expression

Mammalian

Mutation

D119A in aequorin gene

Promoter

IE4/5 promoter (from HSV)

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pET-hisT4DNALig

Plasmid

#216671

Purpose

Expresses his-tagged T4 DNA ligase in E. coli hosts

Depositor

Po-Min Chiang

Article

Hsueh et al PLoS Biol. 2025 Dec 15;23(12):e3003537.

Insert

T4 DNA Ligase (30 T4 phage)

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_AIO-GZV-SynZiFTR_ETV2_Down-Tandem

Plasmid

#241394

Purpose

Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression of ETV2

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

ETV2; ZF10-NS3-p65 (ETV2, RELA, KRAS Human)

Expression

Mammalian

Promoter

ZF10; CAG

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-NFIX

Plasmid

#207192

Purpose

doxycycline-inducible expression of Human NFIX in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-NFIX

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pRP-BRG1-HA-SmBiT

Plasmid

#241815

Purpose

Expresses HA-tagged and HiBiT-tagged BRG1 for use with the Promega HiBiT system

Depositor

Ethan Lee

Article

Kassel et al Nat Commun. 2025 Jun 5;16(1):5248. doi:

Insert

BRG1 (SMARCA4 Human)

Tags

HA and SmBiT

Expression

Mammalian

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-TRE-PFE3-P2A-GFP

Plasmid

#236180

Purpose

AAV version of Tet inducible PFE3 for ablating excitatory synapses.

Depositor

Don Arnold

Article

Bareghamyan et al eLife. 2025 Apr 29;13:RP103757. doi: 10.

Insert

PFE3-P2A-GFP

Use

AAV

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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