159,481 results

1. pLVX-EF1a-GFP-LaminA-IRES-Hygromycin

   Plasmid

   #134867

   Purpose

   Expresses EGFP-tagged nuclear envelope marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Lamin A (LMNA Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. #1.4 post-G-SynapShot-mScarlet

   Plasmid

   #214716

   Purpose

   Expresses green #1.4 post-SynapShot and mScarlet

   Depositor

   Won Do Heo

   Article

   Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

   Insert

   #1.4 post-G-SynapShot
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKDsgRNA-NT

   Plasmid

   #89960

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   empty gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSBtet-GP

   Plasmid

   #60495

   Purpose

   SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and puromycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TCE & RPBSA

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGWB415

   Plasmid

   #74809

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with 3xHA (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXspuro-mCherry-NBR1

   Plasmid

   #74242

   Purpose

   Retroviral expression of mCherry-NBR1

   Depositor

   Jayanta Debnath

   Article

   Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590.

   Insert

   neighbor of BRCA1 gene 1 (NBR1 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3β-FLAG-CBP-HA

   Plasmid

   #32908

   Depositor

   Tso-Pang Yao

   Article

   Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

   Insert

   CBP (Crebbp Mouse)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-R-GECO1.2

   Plasmid

   #45494

   Purpose

   Red intensiometric genetically encoded Ca2+-indicators for optical imaging 1.2

   Depositor

   Robert Campbell

   Article

   Wu et al ACS Chem Neurosci. 2013 Mar 19.

   Insert

   R-GECO1.2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…

   Promoter

   CMV

   Available since

   July 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-mDlx-GFP-Fishell-1

   Plasmid

   #83900

   Purpose

   GFP expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

   Depositor

   Gordon Fishell

   Article

   Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   mDlx

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. epiCRISPR

    Plasmid

    #135960

    Purpose

    Expresses SpCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-CRX-T2A-NeuroD1

    Plasmid

    #194607

    Purpose

    Piggybac Tet-ON plasmid for inducing hiPSCs into photoreceptor-like cells via CRX and NeuroD1 expression.

    Depositor

    Haruhisa Inoue

    Article

    Otsuka et al iScience. 2022 Mar 8;25(4):103987. doi:

    Insert

    CRX-T2A-NeuroD1
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. GST-RBD

    Plasmid

    #15247

    Depositor

    Martin Schwartz

    Article

    Ren et al EMBO J. 1999 Feb 1. 18(3):578-85.

    Insert

    Rhotekin (Rtkn Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 7-89 (Rho binding domain)

    Promoter

    Available since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIRESneo-FLAG/HA Ago2 corrected

    Plasmid

    #10822

    Depositor

    Thomas Tuschl

    Article

    Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    3 silent mutations in orf

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJM253 (miniCTX1-rhaSR-PrhaBAD)

    Plasmid

    #110571

    Purpose

    Integration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoter

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    rhaSR-PrhaBAD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhaSR-PrhaBAD

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJRH-1364 LV EF1a-CD4 IRES-EGFP

    Plasmid

    #201920

    Purpose

    2nd generation lentiviral transfer plasmid for engineering cells to express human CD4

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    CD4 (CD4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHAGE-AXL

    Plasmid

    #116714

    Purpose

    Lentiviral expression of AXL

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    AXL (AXL Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PAM1

    Plasmid

    #198740

    Purpose

    pTFIID-GFP, Geneticin resistance

    Depositor

    Chantal Abergel

    Article

    Philippe et al Nat Protoc. 2023 Nov 14. doi: 10.1038/s4

    Insert

    GFP
    

    Use

    Acanthamoeba expression

    Tags

    Expression

    Mutation

    Promoter

    pTFIID

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-11b_PcrA_M6_H93A

    Plasmid

    #194156

    Purpose

    Engineered PcrA_M6 helicase to support isothermal amplification method SHARP

    Depositor

    Taekjip Ha

    Article

    Gavrilov et al Nat Commun. 2022 Oct 23;13(1):6312. doi:

    Insert

    Engineered helicase PcrA_M6
    

    Use

    Tags

    6x His tag and FLAG tag

    Expression

    Bacterial

    Mutation

    H93A, C96A, N187C, C247A, L384V, L409C

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1 3xHA-KillerRed-OMP25

    Plasmid

    #174544

    Purpose

    localizes KillerRed to mitochondria

    Depositor

    Minna Roh-Johnson

    Article

    Kidwell et al eLife. 2023 Mar 6;12:e85494. doi: 10.755

    Insert

    3xHA-KillerRed-OMP25
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pXPG

    Plasmid

    #71248

    Purpose

    improved luciferase reporter gene plasmid

    Depositor

    Peter Cockerill

    Article

    Bert et al Plasmid. 2000 Sep;44(2):173-82.

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    none

    Available since

    Nov. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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