162,408 results
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Plasmid#227721PurposePlasmid contains T2A 3xNLS GFP, with some nlp-51 homology sequence before, Amp ResistantDepositorInsertT2A 3xNLS GFP with some nlp-51 homology sequence (nlp-51 Nematode)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-acatenin
Plasmid#186458PurposeExpresses human alpha-catenin with an N-terminal GST tag and a PRescission Protease cleavage siteDepositorInsertCTNNA1 (CTNNA1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
DRH015_ AAV-hSyn-WT-THR
Plasmid#225088PurposeExpression of wild-type thyroid receptor beta (THRB, human) with an HA tag in neurons driven by human synapsin promoter.DepositorInsertWT-THRB (THRB Human)
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 mScarletI3-T2A-mTurquoise2
Plasmid#219845Purposeequimolar expression of mScarletI3 and mTurquoise2 in budding yeastDepositorInsertmScarletI3-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 27, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHIV-EGFP.Flag-EZH2-mt1
Plasmid#220244PurposeExpresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.DepositorInsertEZH2 mt 1 (EZH2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationR27A, R31A, F165APromoterEF-1αAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-A'
Plasmid#209115PurposeBacterial expression of CC-GEMS ligand SUMO-A'DepositorInsertSUMO-A'
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
GpACR1-pcDNA3.1-mScarlet
Plasmid#204964PurposeExpression of GpACR1 in fusion with mScarlet in mammalian cellsDepositorInsertGpACR1
UseTagsmScarletExpressionMammalianMutationPromoterCMV (+ enhancer)Available sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
AiP12214 - pAAV-ProB12-SYFP2-WPRE3-BGHpA (Alias: CN2214)
Plasmid#208161PurposeAAV vector for SYFP2 expression in brain astrocytesInsertSYFP2
UseAAVTagsExpressionMutationNAPromoterBeta Globin minimal promoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM33_CLIP_CAP_Gly
Plasmid#178470PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertCLIP_CAP_Gly (CLIP1 Human, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-DEPTOR S286/287/291A
Plasmid#159160PurposeExpresses DEPTOR S286/287/291A mutant in mammalian cellsDepositorInsertDEPTOR (DEPTOR Human)
UseTagsHAExpressionMammalianMutationchanged serine 286,287 and 291 to alaninePromoterCMVAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-DEPTOR S286/287/291D
Plasmid#159161PurposeExpresses DEPTOR S286/287/291D mutant in mammalian cellsDepositorInsertDEPTOR (DEPTOR Human)
UseTagsHAExpressionMammalianMutationchanged serine 286,287 and 291 to aspartic acidPromoterCMVAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
p206_LTJ_sgRNACD45.2_R3
Plasmid#82674PurposesgRNA targeting murine CD45.2 region 3. Co-expresses SpCas9-2A-EGFP.DepositorInsertsgRNA targeting CD45.2 region 3
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
gTBEv5
Plasmid#220621Purposevector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoterDepositorInsertnSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
UseTagsExpressionMammalianMutationhUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…PromoterhU6, EF1aAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSGKP-km
Plasmid#117233PurposeA sgRNA expression plasmid for genome editing in Klebsiella PneumoniaeDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Vinculin-23
Plasmid#55159PurposeLocalization: Focal Adhesions, Excitation: 587, Emission: 610DepositorInsertVinculin (VCL Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLBS-mScarlet
Plasmid#129337Purposecytosol marker, lentivirusDepositorInsertmScarlet (Addgene 85042)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA-HA-human CMYC S62A
Plasmid#203436PurposeMammalian expression of MYCDepositorInsertMYC (MYC Human)
UseTags2 x HAExpressionMammalianMutationS62APromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
3X FLAG-FUS-WT
Plasmid#44985DepositorInsertFUS (Full length) (FUS Human)
UseTags3X FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
FHRE-Luc
Plasmid#1789PurposeReporter plasmid for FOXO3a. Forkhead responsive element with luciferase readout.DepositorInsertFHRE (FOXO3 Human)
UseLuciferaseTagsExpressionMammalianMutation3 copies of FREPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_ENO1-Halo
Plasmid#175330PurposeProduction of HaloTag fusion proteinDepositorInsertENO1 (ENO1 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only