159,461 results

1. piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)

   Plasmid

   #209080

   Purpose

   Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expression

   Depositor

   Vikram Khurana

   Article

   Lam et al bioRxiv 2022.11.08.515615

   Inserts

   SNCA (SNCA Human)
   NGN2 (Neurog2 Mouse)
   

   Use

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   A53T

   Promoter

   TRE4G

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCFD4-U6:1_U6:3tandemgRNAs

   Plasmid

   #49411

   Purpose

   expression of two gRNAs from Drosophila U6:1 and U6:3

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Inserts

   dU6-1:gRNA
   dU6-3:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   dU6-1 and dU6-3

   Available since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. 5HT6-G-GECO1.0

   Plasmid

   #47499

   Purpose

   Fluorescent reporter for Ca2+ inside primary cilia

   Depositor

   Takanari Inoue

   Article

   Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

   Insert

   5-hydroxytriptamine receptor isoform 6 (Htr6 Mouse)
   

   Use

   Tags

   G-GECO1.0

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. p10xQUAS-CsChrimson

   Plasmid

   #163629

   Purpose

   10xQUAS effector expressing CsChrimson.mVenus for red-shifted optogenetic activation of neurons; Contains both P-element ends and attB integration sequence

   Depositor

   Christopher Potter

   Article

   Task et al Elife. 2022 Apr 20;11. pii: 72599. doi:

   Insert

   CsChrimson.mVenus
   

   Use

   Tags

   mVenus

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBV-Luc mut MBS1-4

   Plasmid

   #16565

   Depositor

   Bert Vogelstein

   Article

   Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

   Insert

   c-MYC binding sites (MYC Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Mutant MBS1-4

   Promoter

   Available since

   March 24, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pHR SFFVp PHAkt Cerulean

   Plasmid

   #50837

   Purpose

   Fluorescent reporter for membrane PIP3 levels

   Depositor

   Wendell Lim

   Article

   Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

   Insert

   PHAkt (AKT1 )
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Cerulean

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA6a-mEOS3.2-KanMX6

   Plasmid

   #87030

   Purpose

   Tag S. pombe genes in C terminal with mEOS3.2

   Depositor

   Thomas Pollard, Julien Berro

   Article

   Laplante et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

   Insert

   mEOS3.2
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLVX-IRES-Gαq*-BERKY3

   Plasmid

   #158425

   Purpose

   BRET biosensor for detection of Gαq-GTP in lentiviral vector pLVX-IRES-Hyg

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Lyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. K44A dynamin 1 pEGFP

   Plasmid

   #34681

   Depositor

   Sandra Schmid

   Article

   Song et al Mol Biol Cell. 2004 May;15(5):2243-52. E

   Insert

   dynamin 1ba (DNM1L Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K44A

   Promoter

   Available since

   Jan. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTU2-AmyE-Pveg-eGFP

    Plasmid

    #112792

    Purpose

    Expresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformation

    Depositor

    Chris French

    Article

    Wicke et al bioRxiv 185108

    Insert

    Pveg-eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-ChRger2-TS-EYFP

    Plasmid

    #127237

    Purpose

    High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    ChRger2-TS-EYFP
    

    Use

    AAV

    Tags

    EYFP and SpyTag

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-Synapto-pHluorin

    Plasmid

    #168510

    Purpose

    Expression of Synapto-pHluorin for optical monitoring of presynaptic release in neurons.

    Depositor

    Franck Polleux

    Article

    Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

    Insert

    Synaptophysin-pHluorin (Syp Mouse)
    

    Use

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-HA-Ebp1

    Plasmid

    #67792

    Purpose

    Mammalian expression of Ebp1 tagged with HA

    Depositor

    Stephen Goff

    Article

    Wang et al J Virol. 2014 Feb;88(3):1825-9. doi: 10.

    Insert

    Ebp1 (PA2G4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pMM668

    Plasmid

    #68890

    Purpose

    pExchange(tdk) - B. thetaiotaomicron suicide vector expressing thymidine kinase

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EX-HA-FRB-Cb5_pLX304

    Plasmid

    #120915

    Purpose

    Lentiviral vector expressing EX-HA-FRB-Cb5 on mammalian ER membrane

    Depositor

    Alice Ting

    Article

    Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

    Insert

    EX-HA-FRB-Cb5
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-tdTomato-CNA35

    Plasmid

    #61606

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with tdTomato

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    tdTomato and CNA35
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-EF1a-EGFP-RAB5A-IRES-Puromycin

    Plasmid

    #134858

    Purpose

    Expresses EGFP-tagged early endosome marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Ras-related protein 5A
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mApple-MyosinIIA-C-18

    Plasmid

    #54929

    Purpose

    Localization: Cytoskeleton, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Article

    Burnette et al J Cell Biol. 2014 Apr 14;205(1):83-96. d

    Insert

    MyosinIIA
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pDONR223_ATG16L1_WT

    Plasmid

    #82255

    Purpose

    Gateway Donor vector containing ATG16L1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    ATG16L1 (ATG16L1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-IRES-Gαi*-BERKY3

    Plasmid

    #158424

    Purpose

    BRET biosensor for detection of Gαi-GTP in lentiviral vector pLVX-IRES-Hyg

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
    

    Use

    Lentiviral

    Tags

    myc

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge