163,606 results

1. pci-EGFP-UPF1-WT

   Plasmid

   #135994

   Purpose

   WT UPF1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   UPF1
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT3-EF1aH-PIK3CA H1047R

   Plasmid

   #180031

   Purpose

   This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used in LoxP mice.

   Depositor

   Xin Chen

   Article

   Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv

   Insert

   PIK3CA H1047R (PIK3CA Human)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   H1047R

   Promoter

   EF1a

   Available Since

   March 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-Syn-PSD95-mCherry

   Plasmid

   #125694

   Purpose

   Exogenous expression of PSD95 (red fluorescence)

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   PSD95-mCherry
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available Since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-PGK- dCas9-SunTag-P2A-HygR

   Plasmid

   #193137

   Purpose

   Expresses dCas9 fused to the SunTag scaffold

   Depositor

   Brad Rosenberg

   Article

   Danziger et al PLoS Pathog. 2022 Apr 14;18(4):e1010464.

   Insert

   dCas9-SunTag-T2A-HygR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   PGK

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAK1

   Plasmid

   #73244

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   AAK1 (AAK1 Human)
   

   Use

   Tags

   TEV-His6-Flag

   Expression

   Bacterial

   Mutation

   pfam00069:Pkinase 47-307

   Promoter

   T7

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

6. OPEN Beta2-microglobulin

   Plasmid

   #215657

   Purpose

   E. coli expression of human beta-2-microglobulin containing a cysteine mutation for disulfide linkage to mutant HLA.

   Depositor

   Nikolaos Sgourakis

   Article

   Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

   Insert

   OPEN Beta2-microglobulin (B2M Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Histidine 31 to Cysteine

   Promoter

   T7

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP

   Plasmid

   #86457

   Purpose

   Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites

   Depositor

   Ralf Kuehn

   Article

   Chu et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

   Insert

   BFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Pgk

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. plk1 WT GFP-myc

   Plasmid

   #132963

   Purpose

   To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

   Depositor

   Michael Yaffe

   Article

   Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

   Insert

   polo-like kinase 1 (PLK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMH-HA

   Plasmid

   #101767

   Purpose

   Gateway-compatible destination vector for mammalian expression of N-terminal HA-tag fusion protein

   Depositor

   Michael Huen

   Article

   An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

   Type

   Empty backbone

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV HA-DP-1

    Plasmid

    #133705

    Purpose

    Expression of HA-tagged TFDP1.

    Depositor

    James A. DeCaprio

    Article

    Krek et al Science. 1993 Dec 3. 262(5139):1557-60.

    Insert

    TFDP1 (TFDP1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSPIH6

    Plasmid

    #190676

    Purpose

    E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)

    Depositor

    John Vederas

    Article

    Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi

    Inserts

    Small ubiquitin-like modifier
    Mxe GyrA intein with C-terminal chitin binding domain and His tag
    

    Use

    Tags

    Chitin binding domain, His tag, and N-terminal Hi…

    Expression

    Bacterial

    Mutation

    Added C-terminal his tag and None

    Promoter

    T7

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLibacceptorV2

    Plasmid

    #106250

    Purpose

    Recombination-mediated cassette exchange with target genomic loci

    Depositor

    Sriram Kosuri

    Article

    Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDNA3.1-hMcl-1

    Plasmid

    #25375

    Depositor

    Roger Davis

    Article

    Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

    Insert

    MCL1 (MCL1 Human)
    

    Use

    Tags

    V5 and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. Rosa26 mT/mG

    Plasmid

    #17787

    Depositor

    Liqun Luo

    Article

    Muzumdar et al Genesis. 2007 Sep . 45(9):593-605.

    Insert

    Rosa26 mT/mG
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    membrane tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. hBax C3-EGFP

    Plasmid

    #19741

    Depositor

    Richard Youle

    Article

    Nechushtan et al EMBO J. 1999 May 4. 18(9):2330-41.

    Insert

    Bax (BAX Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available Since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pICE-HA-MRE11-WT

    Plasmid

    #82033

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of wild-type human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    MRE11 (MRE11 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-tet

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. His-Tev-Nedd4L

    Plasmid

    #186806

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-Nedd4L.

    Depositor

    Lalit Deshmukh

    Article

    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

    Insert

    His-Tev-Nedd4L (NEDD4L Human)
    

    Use

    Tags

    His6-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pΔΦ

    Plasmid

    #60009

    Purpose

    Deleted foamy virus vector plasmid containing a backbone with a polylinker region for insertion of a transgene cassette. Ampicillin resistant.

    Depositor

    David Russell

    Article

    Trobridge et al Mol Ther. 2002 Sep;6(3):321-8.

    Type

    Empty backbone

    Use

    Deleted foamy virus

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 Neo(+) hSUMF1

    Plasmid

    #24386

    Depositor

    Carolyn Bertozzi

    Article

    Wu et al Proc Natl Acad Sci U S A. 2009 Mar 3. 10

    Insert

    hSUMF1 (SUMF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. mTurquoise2

    Plasmid

    #198196

    Purpose

    Spectral correction: Expression of mTurquoise2

    Depositor

    Christian Wahl-Schott

    Article

    Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A206K

    Promoter

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge