159,352 results

1. pLV puro Tet VSVG

   Plasmid

   #82724

   Purpose

   Lentiviral plasmid that expresses VSVG protein under a doxycycline-inducible promoter

   Depositor

   Warren Zipfel

   Insert

   VSVG
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBP-P43

   Plasmid

   #112775

   Purpose

   Strong promoter activity during exponential and stationary phase

   Depositor

   Chris French

   Article

   Wicke et al bioRxiv 185108

   Insert

   P43 promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. p8327 pLIX YAP1 S127A

   Plasmid

   #184528

   Purpose

   Expression of YAP1 S127A

   Depositor

   Elizabeth White

   Article

   Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

   Insert

   YAP1 S127A (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   S127A

   Promoter

   TRE promoter, Tet ON

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-REPORT(PGKCMV)-nucTomato-hygro

   Plasmid

   #208573

   Purpose

   LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an PGK/CMV promoter driving nuclear d2eGFP E2A Hygromycin

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   mnucTomato
   d2nucEGFP
   HygroR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK/CMV and minP

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF1680

   Plasmid

   #142824

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   GTF2IRD2B (GTF2IRD2B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV_PGK_LTB4R

   Plasmid

   #208087

   Purpose

   Mammalian expression of the wild-type human LTB4 receptor LTB4R.

   Depositor

   Sean R Collins

   Article

   Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

   Insert

   LTB4R (LTB4R Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   PGK

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. hsKIF5B(1-560)-HaloTag-FRB

   Plasmid

   #213899

   Purpose

   Imaging of KIF5B fused to HaloTag

   Depositor

   Lukas Kapitein

   Insert

   hsKIF5B(aa1-560)-HaloTag-FRB (KIF5B Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. M13cp-dg3 hp

   Plasmid

   #218095

   Purpose

   Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pIII

   Depositor

   Kevin Esvelt

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Active Wnt10A-V5

   Plasmid

   #43822

   Depositor

   Xi He

   Article

   MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

   Insert

   WNT10A (WNT10A Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-Peroxisomes-0

    Plasmid

    #54501

    Purpose

    Localization: Peroxisomes, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Type

    Empty backbone

    Use

    Tags

    EGFP and Peroximal Targeting Signal 1 (PTS1)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pBAC-lacZ

    Plasmid

    #13422

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. T7-RelA(S536E)

    Plasmid

    #24156

    Depositor

    Warner Greene

    Article

    Chen et al Mol Cell Biol. 2005 Sep . 25(18):7966-75

    Insert

    RelA (RELA Human)
    

    Use

    Tags

    T7 gp10

    Expression

    Mammalian

    Mutation

    S536E

    Promoter

    Available since

    April 29, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pRK5-Myc-Parkin

    Plasmid

    #17612

    Depositor

    Ted Dawson

    Article

    Zhang et al Proc Natl Acad Sci U S A 2000 Nov 21;97(

    Insert

    Parkin (PRKN Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. wtBxb1

    Plasmid

    #222337

    Purpose

    Plasmid for expressing wild type Bxb1 in mammalian cells

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized wild type Bxb1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET246-pUC57 12xMS2V6

    Plasmid

    #104390

    Purpose

    MS2V6 cloning

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    12xMS2V6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. EF1a-dCas9-KRAB-GFP backbone

    Plasmid

    #194281

    Purpose

    Vector for CRISPRi-GFP expression ready for gateway cloning of sgRNA

    Depositor

    Elizabeth Kirby

    Article

    Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

    Insert

    dCas9-KRAB-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-hNox3

    Plasmid

    #58341

    Purpose

    expresses human Nox3 in mammalian cells

    Depositor

    Botond Banfi

    Article

    Kiss et al Curr Biol. 2006 Jan 24;16(2):208-13.

    Insert

    NADPH oxidase 3 (NOX3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGFP Cortactin

    Plasmid

    #50728

    Purpose

    mammalian expression of GFP-Cortactin. Note- This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

    Depositor

    Kenneth Yamada

    Insert

    Cortactin (CTTN Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. TFORF2236

    Plasmid

    #141982

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GATA4 (GATA4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV_Anc689-NG-nCas9

    Plasmid

    #163527

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    Anc689-NG-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Promoter

    Available since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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