173,051 results

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pMJ920

Plasmid

#42234

Depositor

Jennifer Doudna

Article

Jinek et al elife. 2013;2:e00471. doi: 10.7554/eLife

Insert

Cas9

Use

CRISPR

Tags

GFP, HA epitope, and NLS (nuclear localization si…

Expression

Mammalian

Mutation

codon-optimized synthetic DNA sequence

Promoter

CMV

Available Since

March 1, 2013

Availability

Academic Institutions and Nonprofits only

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pWF079_LVt-EF1a-TCRGibson_humanTRAC

Plasmid

#233448

Purpose

Gibson assembly of TCRB-P2A-TCRa

Depositor

William Freed-Pastor

Article

Ely et al Science. 2025. May 8; 388(6747)

Type

Empty backbone

Use

Lentiviral

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCSFLPe

Plasmid

#31130

Depositor

Gerhart Ryffel

Article

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

FLP recombinase

Expression

Mammalian

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pET28a T7pCONS TIR-2 sfGFP

Plasmid

#154464

Purpose

pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

Depositor

Daniel Daley

Article

Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

Insert

super folder GFP

Tags

His-thrombin

Expression

Bacterial

Promoter

T7

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pSpCTRE-CD4

Plasmid

#114010

Purpose

Dox-inducible Cas9 expression with a CD4 selection marker

Depositor

Charles Rudin

Article

Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

Insert

SpCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDN41

Plasmid

#61344

Purpose

mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES)

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Tags

GS linker-AsLov2-biNLS9 and PKIt NES

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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3xnls-mScarlet-I

Plasmid

#98816

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

3xNLS

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB2337

Plasmid

#67555

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Expression

Yeast

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pHCMV_ISFV_G

Plasmid

#207282

Purpose

Mammalian expression of Isfahan virus G protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

ISFV_G

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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ActTS-GR

Plasmid

#79774

Purpose

FRET-based actinin tension sensors

Depositor

Tetsuya Kitaguchi

Article

Yamashita et al Sci Rep. 2016 Jun 23;6:28535. doi: 10.10

Insert

ActTS-GR

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-CD19.28H.28TM.28.BB.3z

Plasmid

#200680

Purpose

Modular CD28hinge-CD28transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Michele Bernasconi

Article

Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

Insert

FMC63-CD28HD-CD28TM-CD28-41BB-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)

Use

Lentiviral

Tags

MycTag

Expression

Mammalian

Promoter

EF1a-short

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA

Plasmid

#168290

Purpose

CRISPR-activator Suntag-P65-HSF1 (SPH)

Depositor

Hui Yang

Article

Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

Insert

dCas9, Suntag-P65-HSF1

Expression

Mammalian

Promoter

CAG

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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LRG (Lenti_sgRNA_EFS_GFP)

Plasmid

#65656

Purpose

Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.

Depositor

Christopher Vakoc

Article

Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter-driven sgRNA expression and EFS promo…

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SIRT6_H133Y

Plasmid

#53149

Purpose

expression vector for catalyitc inactive human Sirtuin6

Depositor

Katrin Chua

Article

Michishita et al Nature. 2008 Mar 27;452(7186):492-6. doi

Insert

Sirt6 (SIRT6 Human)

Tags

FLAG

Expression

Mammalian

Mutation

H133Y, catalytic inactive

Promoter

CMV

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
GFP-ESR1

Plasmid

#65211

Purpose

Full length mammalian expression vector for hESR1

Depositor

Ratna Vadlamudi

Insert

esr1 (ESR1 Human)

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3

Plasmid

#48743

Purpose

Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +1060 with respect to transcription start site at +1)

Depositor

Joseph Takahashi

Article

Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

Insert

truncated mPer2 promoter/enhancer region (-1128 to +1060)

Use

Luciferase

Mutation

truncated promoter region (see pGL6)

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only
MLF2-GFP-3xFLAG

Plasmid

#230986

Purpose

Expresses GFP tagged MLF2 in mammalian cells

Depositor

Christian Schlieker

Article

Prophet et al Nat Cell Biol. 2022 Nov;24(11):1630-1641

Insert

MLF2 (MLF2 Human)

Tags

3xFLAG and GFP

Expression

Mammalian

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti SpBsmBI sgRNA Hygro

Plasmid

#62205

Purpose

An empty sgRNA expression vector for expression of sgRNA for Sp Cas9

Depositor

Rene Maehr

Article

Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pIRES-hrGFP II-TET1

Plasmid

#83568

Purpose

Expresses TET1 in mammalian cells

Depositor

Jean-Pierre Issa

Article

Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

Insert

Tet Methylcytosine Dioxygenase 1 (TET1 Human)

Tags

3X FLAG tag and hr GFP II

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pAL750 :: msfGFP

Plasmid

#250661

Purpose

Inducible promoter Pxyl expressing msfGFP in haloarcheon Haloferax volcanii

Depositor

Alexandre Bisson

Article

Rados et al Front Microbiol. 2023 Aug 10;14:1204876.

Insert

Monomeric Superfolder Green Fluorescent Protein

Use

Haloarchaeal expression

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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