179,844 results
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Plasmid#64924PurposeExpresses V5- and SBP-tagged hnRNPM in mammalian cellsDepositorAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by
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GFP-ESR1
Plasmid#65211PurposeFull length mammalian expression vector for hESR1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertesr1 (ESR1 Human)
TagsAcGFPExpressionMammalianPromoterCMVAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-CRY2-iSH2
Plasmid#66839PurposeExpression of a light-inducible PI3-kinase, generated by fusing the iSH2 domain of human p85α (residues 420–615) to mCherry-CRY2. Binds endogenous PI3-kinase p110α catalytic subunits constitutively.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertiSH2
TagsmCherry-CRY2ExpressionMammalianPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Syn-FLEX-rc[Chronos-GFP]
Plasmid#62722PurposeAAV-mediated expression of Chronos-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.DepositorInsertChronos-GFP
UseAAVTagsGFPExpressionMammalianPromoterhuman synapsin promoterAvailable sinceApril 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-TO-nls-st1dCas9-3nls-3XGFP-2nls
Plasmid#64113PurposeExpression of St1 dCas9-3XGFP in mammalian cellsDepositorInsertSt1 dCas9
UseCRISPR and LentiviralTags3XsfGFPExpressionMammalianPromoterCMV-TOAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
YFP-Parkin-IRES-zeo
Plasmid#61728PurposeExpression of human Parkin fused to eYFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertParkin (PRKN Human)
TagseYFPExpressionMammalianAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJW1512
Plasmid#62366PurposeN-terminal 3X FLAG fused to wt BirA - mCherry - sec61ßDepositorInsert3xFLAG-BirA-mCherry-Sec61beta tail anchor (SEC61B Human)
UseLentiviralExpressionMammalianPromoterpTRE2Available sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-UbC
Plasmid#62806PurposeTo express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.DepositorTypeEmpty backboneUseAAV; EucaryoticExpressionMammalianPromoterhUbCAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-ICUE3
Plasmid#61623PurposePlasma membrane-targeted FRET biosensor for monitoring cAMPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLyn-ICUE3 (RAPGEF3 Human)
TagsECFP, N-terminal targeting sequence from Lyn kina…ExpressionMammalianPromoterCMVAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOPINK-Cezanne (OTU, aa 53-446)
Plasmid#61581PurposeExpresses human Cezanne (OTU domain) in E. coli.DepositorInsertCezanne (OTUD7B Human)
TagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-52 and aa 447-843.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
eGFPbait-E2A-KalTA4-pA donor vector
Plasmid#61069Purposefor CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in combination with a eGFP specific sgRNA (e.g. Plasmid 61051)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertseGFPbait
KalTA4
UseZebrafish expressionTagsE2AAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSBbi-RH
Plasmid#60516PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and hygromycin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBUN411
Plasmid#50581PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistanceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserts3×FLAG-NLS-zCas9-NLS
gRNA scaffold
UseCRISPR; Plant expressionTags3xFLAG and NLSMutationmaize codon optimizedPromoterOsU3p and Ubi1pAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV5-25BoxB
Plasmid#60817PurposeCloning vector, 25 repeats of 19 bp BoxB sequence from bacteriophage-λ, for RNA labeling. BoxB-RNA is bound by 22 amino acid RNA binding domain of λ-anti-terminator protein N, e.g. tagged with GFPDepositorInsert25x BoxB
UseSynthetic BiologyExpressionMammalianMutation25 repeats of BoxB sequencePromoterCMVAvailable sinceNov. 16, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FUW-tetO-loxP-hKLF2
Plasmid#60850PurposeLentiviral DOX-inducible expression vector for human KLF2DepositorInsertKLF2 (KLF2 Human)
UseLentiviralAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG_mRuby2_smFP OLLAS
Plasmid#59761Purposeencodes multiple copies of an epitope tag OLLAS ( not detected by anti GFP ab)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmRuby2_smFP_OLLAS
ExpressionMammalianPromoterCAGAvailable sinceOct. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NUP50-N-10
Plasmid#55111PurposeLocalization: Nuclear Pore, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNUP50 (NUP50 Human)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLX304-YAP1(S94A)
Plasmid#59145PurposeYAP1 serine 94 o alanine mutants which disrupts binding to TEAD transcription factorsDepositorAvailable sinceAug. 18, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-Intersectin Long
Plasmid#47395DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertintersectin long (ITSN1 Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCS-H2B-cerulean
Plasmid#53748PurposeContains a fusion of human histone H2B to cerulean fluorescent protein in the pCS2+ vector.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthistone H2B (H2BC21 Human)
TagsCeruleanExpressionMammalianPromotersimian CMV IE94Available sinceJuly 8, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by