161,641 results

1. pAAV-Ef1a-fDIO EYFP

   Plasmid

   #55641

   Purpose

   Flp-Dependent EYFP

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   EYFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-X1-Neo-GFP-FAM134B-LIRmut

   Plasmid

   #109027

   Purpose

   stable lentiviral expression of cDNA

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   FAM134B-LIRmut (RETREG1 Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   DDFELL to DDAEAL aa 453-458 to interrupt FAM134B …

   Promoter

   EIF-1a promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. UAS-mCherry

   Plasmid

   #135457

   Purpose

   Mammalian mCherry reporter to read out FLARE and SPARK activation

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   UAS

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFUW-tetO-Myb

   Plasmid

   #139826

   Purpose

   doxycycline-inducible expression of mouse Myb in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   Myb (Myb Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   P94H and Q619R- Please see depositor comment belo…

   Promoter

   TRE-mCMV

   Available since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. CL20_mEGFP_EWSR1_ERG

   Plasmid

   #205819

   Purpose

   Express mEGFP-tagged fusion protein, EWSR1_ERG from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   EWSR1_ERG (EWSR1, ERG Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGGDestTol2LC-1sgRNA

   Plasmid

   #64239

   Purpose

   destination vector for one U6x:sgRNA cassette

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Str-KDEL_SBP-EGFP-GPI

   Plasmid

   #65294

   Purpose

   synchronize trafficking of EGFP-GPI from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   GPI anchor
   

   Use

   Tags

   EGFP and IL-2 Signal Sequence and Streptavidin Bi…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGART-mCherry

   Plasmid

   #167164

   Purpose

   Expresses human GART-mCherry fusion in mammalian cells

   Depositor

   Stephen Benkovic

   Insert

   GART (GART Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmyc-GFP-TNRC6A-(1-1223)

   Plasmid

   #42013

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   EGFP and Myc

   Expression

   Mammalian

   Mutation

   contain aa1-1223

   Promoter

   CMV

   Available since

   March 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. TtGNN-GFP-empty

    Plasmid

    #106380

    Purpose

    dox inducible expression of GFP with neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-tight

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO_STf_LamB1

    Plasmid

    #181999

    Purpose

    CMV overexpression of SNAP-tag fast fusion to Lamin B1 for nuclear envelope localization in mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    STf-LamB1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. 1b34

    Plasmid

    #177692

    Purpose

    HCV E1E2 expression plasmid

    Depositor

    Justin Bailey

    Article

    Salas et al Gastroenterology. 2022 Feb;162(2):562-57

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0

    Plasmid

    #174081

    Purpose

    Expression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.

    Depositor

    Ryan Mehl

    Article

    Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-

    Insert

    NES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
    

    Use

    Tags

    NES-Flag

    Expression

    Mammalian

    Mutation

    L270G, Y271L, N311G, C313A

    Promoter

    CMV

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. p-dCas9-SSAP-MS2-BB_BbsI

    Plasmid

    #183826

    Purpose

    pU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFP

    Depositor

    Le Cong

    Article

    Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

    Insert

    U6-guideRNA-dCas9-T2A-EBFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBH

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-FLT3-V5/HIS

    Plasmid

    #201985

    Purpose

    expression of human FLT3 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    FLT3 receptor tyrosine kinase (FLT3 Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. HcKCR1_mCherry_pcDNA3.1

    Plasmid

    #177336

    Purpose

    Expresses KCR1 in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

    Insert

    HcKCR1
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    mammalian codon-adapted

    Promoter

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST-CMV-C-EGFP

    Plasmid

    #122844

    Purpose

    C-terminal EGFP tag. Gateway destination vector for mammalian expression.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Plxnb2(L)-Fc-His

    Plasmid

    #72128

    Purpose

    Expresses the extracellular region of the PlexinB2 protein (ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Plxnb2 (Plxnb2 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCherry-Rab9a

    Plasmid

    #78592

    Purpose

    Express mCherry-Rab9a in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    Rab9a (RAB9A Human)
    

    Use

    Tags

    mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA

    Plasmid

    #55201

    Purpose

    Plasmid encoding the Ribozyme/gRNA architecture. This is a modified form of the original plasmid described in the paper (Construct 13). mKate2 was replaced with dsRed2 because of distribution issues.

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    dsRed2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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