163,606 results

1. pGL3 RARE-RFP

   Plasmid

   #183054

   Purpose

   Fluorescent reporter for Retinoic Acid activity

   Depositor

   Chaya Kalcheim

   Article

   Rekler et al Elife. 2022 Apr 8;11. pii: 72723. doi: 1

   Insert

   RFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   RARE, SV40

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. dCas9-CBP FL

   Plasmid

   #179550

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of full length human CBP driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with c-terminal fusion of full length human CBP (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. His6-MBP-uTEV3

   Plasmid

   #135464

   Purpose

   Bacterial expression of uTEV3 (full-length) protease

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   His6-MBP-uTEV3
   

   Use

   Tags

   MBP, His6

   Expression

   Bacterial

   Mutation

   S219V mutation (improves stability) , I138T/S153N…

   Promoter

   T5

   Available Since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMitoLOC

   Plasmid

   #58980

   Purpose

   Expression of preCOX4-mCherry & preSU9-GFP for colocalisation analysis of yeast mitochondrila

   Depositor

   Markus Ralser

   Article

   Vowinckel et al Mitochondrion. 2015 Sep;24:77-86. doi: 1

   Inserts

   mCherry
   GFP
   

   Use

   Tags

   preCOX4 and preSU9

   Expression

   Yeast

   Mutation

   Promoter

   ADH1 and MET17

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGP-CMV-jGCaMP7f

   Plasmid

   #104483

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Insert

   jGCaMP7f
   

   Use

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-Tol2

   Plasmid

   #31823

   Depositor

   Stephen Ekker

   Article

   Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

   Insert

   Tol2 Transposase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV_Xph15-GCaMP6f

   Plasmid

   #135538

   Purpose

   Encodes a specific PSD-95 binder (Xph15) fused to GCaMP6f

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph15
   

   Use

   Tags

   GCaMP6f

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. BFP-Rab5

   Plasmid

   #49147

   Purpose

   mammalian expression of BFP-Rab5 fusion

   Depositor

   Gia Voeltz

   Article

   Friedman et al Mol Biol Cell. 2013 Apr;24(7):1030-40. d

   Insert

   Rab5 (RAB5B Human)
   

   Use

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-NLS-copGFP-EF1-BlastiS

   Plasmid

   #132772

   Purpose

   Spills into the cytoplasm during nuclear envelope rupture.

   Depositor

   Jan Lammerding

   Article

   Denais et al Science. 2016 Apr 15;352(6283):353-8. do

   Insert

   NLS-copGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLENTI_RPS3_Keima

    Plasmid

    #127140

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    RPS3_Keima (RPS3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK5_mCherry_P2AT2A_GFP-nb-2xKS_FtoG

    Plasmid

    #238237

    Purpose

    For overexpression of mCherry_P2AT2A_GFP-nb-2xKS_FtoG

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry_P2AT2A_GFP-nb-2xKS_FtoG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pdest METTL1-WT Neo

    Plasmid

    #223058

    Purpose

    METTL1 gene expression in mammalian cells

    Depositor

    Alejandro Gutierrez

    Article

    Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

    Insert

    METTL1 (METTL1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-PBD-EYFP

    Plasmid

    #13723

    Depositor

    Klaus Hahn

    Article

    Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

    Insert

    PBD-EYFP (PAK1 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    p21 binding domain of human Pak1(amino acids 65-1…

    Promoter

    Available Since

    March 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)-FluoSTEP-ICUE

    Plasmid

    #181845

    Purpose

    Genetically encoded FRET-based sensor for monitoring cAMP dynamics near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    FluoSTEP-ICUE (RAPGEF3 Human)
    

    Use

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pL-CRISPR.EFS.GFP

    Plasmid

    #57818

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    EFS (EEF1A1 Human)
    P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    EFS and hU6

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3β-FLAG-CBP-HA

    Plasmid

    #32908

    Depositor

    Tso-Pang Yao

    Article

    Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

    Insert

    CBP (Crebbp Mouse)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSCVpuro-Cre

    Plasmid

    #34564

    Purpose

    Retroviral expression of Cre

    Depositor

    Kai Ge

    Article

    Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

    Insert

    Cre
    

    Use

    Cre/Lox and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available Since

    Jan. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. dSaCas9-VPR

    Plasmid

    #205251

    Purpose

    In vitro transcription plasmid for production of dSaCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #68495

    Depositor

    Rasmus Bak

    Article

    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

    Insert

    dSaCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. p1321 HPV-16 E6/E7

    Plasmid

    #8641

    Depositor

    Peter Howley

    Article

    Münger et al J Virol. 1989 Oct . 63(10):4417-21.

    Insert

    HPV-16 E6/E7 (E7, E6 Human papillomavirus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 16, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3 AIF 1-90 mCherry FRB

    Plasmid

    #67530

    Purpose

    For expression of mCherry fused FRB to the mitochondrial innermembrane

    Depositor

    Stephen Tait

    Article

    Ichim et al Mol Cell. 2015 Mar 5;57(5):860-72. doi:

    Inserts

    AIF a.a. 1 - 90
    mCherry
    FRB
    

    Use

    Tags

    AIF 1 - 90, FRB, and mCherry

    Expression

    Mammalian

    Mutation

    T->L

    Promoter

    CMV

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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