159,352 results

1. pFA6a-link-yomRuby2-Kan

   Plasmid

   #44953

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mRuby2 with Kan selection

   Depositor

   Kurt Thorn, Wendell Lim

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomRuby2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-BCL6-IRES-GFP

   Plasmid

   #31391

   Depositor

   Hilda Ye

   Article

   Yu et al Blood. 2005 Feb 15. 105(4):1777-84.

   Insert

   B-cell lymphoma 6 (BCL6 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. MTK3_038

   Plasmid

   #123752

   Purpose

   Encodes R-Flinc-A, a cAMP reporter, as a Type 3 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   R-FlincA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTPGI_dCas9_VP64

   Plasmid

   #49013

   Purpose

   encodes yeast-optimized dCas9_VP64 synthetic transcription factor

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   pTPGI (galactose+aTc inducible)

   Available since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMZ7

   Plasmid

   #223180

   Purpose

   pORI28 derivative with lacZ and upp gene inserted into the backbone; for generating gene-specific knockouts.

   Depositor

   Paul Blainey

   Article

   Zhu et al bioRxiv [Preprint]. 2023 Dec 30:2023.12.

   Type

   Empty backbone

   Use

   Chromosomal integration

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. mTq2-Calmodulin

   Plasmid

   #198200

   Purpose

   FRET donor: Calmodulin-2 tagged with N-terminal mTurquoise2

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   Calmodulin 2 (Calm2 Rat)
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA 3 5' cMyc SMC1 wt

   Plasmid

   #32363

   Depositor

   Michael Kastan

   Article

   Kim et al Genes Dev. 2002 Mar 1;16(5):560-70.

   Insert

   SMC1A (SMC1A Human)
   

   Use

   Tags

   c-Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNESx2-EGFP-C1-PABDx2-Spo20

   Plasmid

   #220082

   Purpose

   PA biosensor with tandem Spo20-PABDs and two NES domains to aid in nuclear export

   Depositor

   Gerry Hammond

   Article

   Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

   Insert

   Spo20 (SPO20 Budding Yeast)
   

   Use

   Tags

   X. leavis map2k1.L(32-44)-GGSG-X. leavis map2k1.L…

   Expression

   Mammalian

   Mutation

   amino acids 51-91, tandem dimer

   Promoter

   CMV

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. mTurquoise2

   Plasmid

   #198196

   Purpose

   Spectral correction: Expression of mTurquoise2

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A206K

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZac2.1 gfaABC1D-Ezr-eGFP

    Plasmid

    #176856

    Purpose

    Expresses Ezrin fused with eGFP at the C-terminus in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    Ezr-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    gfaABC1D

    Available since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKI1.1R

    Plasmid

    #85808

    Purpose

    CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

    Depositor

    Tetsuya Higashiyama

    Article

    Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

    Insert

    human-codon-optimized SpCas9
    

    Use

    CRISPR

    Tags

    FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    AtRPS5A

    Available since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBAD24-sfGFPx2

    Plasmid

    #51559

    Purpose

    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)

    Depositor

    Sankar Adhya, Francisco Malagon

    Article

    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

    Insert

    superfolder GFP
    

    Use

    Tags

    GLESTCRHASLAVLADERRFSA and MARARA

    Expression

    Bacterial

    Mutation

    Contains additonal aa in the N and C terminus (in…

    Promoter

    Available since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP SMG1 WT

    Plasmid

    #199571

    Purpose

    Expresses WT SMG1 fused to GFP

    Depositor

    Michael Kastan

    Article

    McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

    Insert

    SMG1 WT (SMG1 Human)
    

    Use

    Tags

    GFP fusion protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. DsRed-rab7 DN

    Plasmid

    #12662

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB7 (Homo sapiens) (RAB7A Human)
    

    Use

    Tags

    DsRed and HA

    Expression

    Mammalian

    Mutation

    T22N

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Drp1K38A

    Plasmid

    #45161

    Depositor

    Richard Youle, Alexander van der Bliek

    Article

    Smirnova et al J Cell Biol. 1998 Oct 19;143(2):351-8.

    Insert

    Drp1 (DNM1L Human)
    

    Use

    Tags

    HA (AYPYDVPDY)

    Expression

    Mammalian

    Mutation

    K38A

    Promoter

    CMV

    Available since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF2652

    Plasmid

    #142969

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ARNTL2 (BMAL2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLminP_Luc2P_RE9

    Plasmid

    #90343

    Purpose

    HIF1a - Hypoxia gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    HIF1a

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. PTGER2-Tango

    Plasmid

    #66484

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    PTGER2 (PTGER2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRXIP-GFP-PI4K2A

    Plasmid

    #221760

    Purpose

    Stable expression of GFP-PI4K2A in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    PI4K2A (PI4K2A Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-FAST-p10

    Plasmid

    #89152

    Purpose

    Express p10-FAST in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    NBV p10-FAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promotor

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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