-
Plasmid#112510Purposehuman Ah receptor and Arnt cDNAs expressed under control of Gal1,10 bidirectional promoterDepositorUseSynthetic BiologyTagsExpressionYeastMutationPromoterGal1,10Available sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only
-
-
pCW57-RFP-P2A-MCS
Plasmid#78933PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.DepositorTypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTight TRE promoterAvailable sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
3XFlagNICD1
Plasmid#20183DepositorInsertNotch 1 Intracellular Domain (Notch1 Mouse)
UseTags3XFLAGExpressionMammalianMutationContains murine Notch 1 Intracellular Domain (Val…PromoterCMVAvailable sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
sgRNA.SFFV.tRFP
Plasmid#169941PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and tRFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-MTS-TagBFP-P2AT2A-EGFP-NLS-P2AT2A-mCherry-PTS1
Plasmid#87829PurposeHigh-efficient mammalian expression vector for co-expression of BFP-, EGFP- and mCherry-tagged proteins using P2AT2A. MTS, NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsMTS
NLS
PTS1
UseTagsExpressionMammalianMutationPromoterCMV promoter, tetracycline operatorAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
CRY2olig-FUS
Plasmid#218921PurposeExpression of CRY2olig-mCherry fused to FUSDepositorInsertCRY2olig-mCherry tagged FUS
UseTagsExpressionMammalianMutationwild-typePromoterCMVAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPING
Plasmid#26046DepositorInsertSS peptide-LacZ-KHHHHHH
UseTagsKHHHHHH and MGILPSPGMPALLSLVSLLSVLLMGCVAETGExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceMay 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-EGFPKASH
Plasmid#124882Purposelabelling of neuronal nuclear envelope with EGFPDepositorInsertEGFP
UseAAVTagsKASHExpressionMutationPromoterCaMKIIAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP::L4440
Plasmid#11335DepositorInsertGFP
UseRNAiTagsExpressionWormMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
TFORF2882
Plasmid#143653PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertXBP1 (XBP1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-RhoA-T19N
Plasmid#12967DepositorInsertRhoA DN (RHOA Human)
UseTagsEGFPExpressionMammalianMutationT19N Dominant negativePromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-NLS-mEGFP
Plasmid#105527PurposeT7 promotor drives in vitro mRNA transcription of a mEGFP with a nuclear localisation signalDepositorInsertNucleoplasmin
UseIn vitro transcriptionTagsmEGFPExpressionMutationNLS of nucleoplasmin (encoding amino acids KRPAAT…PromoterT7Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
AiP13897 - pAAV-AiE0671m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3897)
Plasmid#224038PurposeAiE0671m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L4/5_IT neuronsInsertSYFP2
UseAAVTagsExpressionMutationNAPromoterminBGAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2F
Plasmid#15800DepositorInsertUbiquitin conjugating enzyme UbE2F (UBE2F Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-cathepsinD-YMH
Plasmid#182644PurposeMammalian expression vector encoding a protein fusion composed of human cathepsin D fused to to one tyrosine sulfation (TS) motif (Y), myc (M) and His6 (H) epitopeDepositorInsertcathepsinD
UseRetroviralTagsHis6-tag, TS site from proCCK, and myc-tagExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_PIK3CA_p.E545K
Plasmid#82881PurposeGateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.DepositorInsertNM_006218 (PIK3CA Human)
UseGateway entry vectorTagsExpressionMutationE545KPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Halo-Tev-Flag-Ube2v2
Plasmid#110070PurposeExpress Halo-Tev-Flag-Ube2v2 (human) in mammalian cellsDepositorInsertHalo-Tev-Flag-Ube2v2 (UBE2V2 Human)
UseTagsHalo-TEV-FlagExpressionMammalianMutationPromoterT7Available sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTol1-UAS:GtACR2-tdTomato
Plasmid#124236PurposeZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin GtACR2 fused to the red fluorescent protein tdTomatoDepositorInsertGtACR2-tdTomato
UseZebrafish expressionTagstdTomatoExpressionMutationPromoter14xUAS-E1bAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJR019
Plasmid#198834PurposeInactivation of G protein subunits through Pertussis toxin (PTX)DepositorInsert15xUAS::PTX-SL2-GFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only