163,606 results

1. pHAGE2 mCherry-Atl3

   Plasmid

   #86680

   Purpose

   Lentivirus to stably express fluorescent protein in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

   Insert

   ATL3 (ATL3 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Geminin-mStrawberry reporter

   Plasmid

   #158690

   Purpose

   This plasmid is a Geminin cell cycle reporter. It has CMV promoter driving the expression of Geminin fused to mStrawberry-pGK-BSD

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   mStrawberry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pScaf-3024.1

   Plasmid

   #111404

   Purpose

   pScaf phagemid with insert for generating DNA origami scaffold 3024 bases in length

   Depositor

   Shawn Douglas

   Article

   Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

   Insert

   pScaf-3024
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. phCMV-229E-D15

   Plasmid

   #188908

   Purpose

   express truncated 229E spike for pseudovirus production

   Depositor

   David Nemazee

   Insert

   229E spike (S 229E)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   c-terminal 15aa deletion

   Promoter

   CMV

   Available Since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-miniDq-mCherry-P2A-HA-KORD

   Plasmid

   #204359

   Purpose

   AAV vector for coexpression of miniDq and KORD under the control of human synapsin promoter

   Depositor

   Masao Doi

   Article

   Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

   Insert

   miniDq-mCherry-P2A-HA-KORD
   

   Use

   AAV

   Tags

   HA (for KORD) and mCherry (for miniDq)

   Expression

   Mutation

   For miniDq, the third intracellular loop (ICL3) o…

   Promoter

   hSyn

   Available Since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMT1-24X-NOG(AtUBI10)

   Plasmid

   #202016

   Purpose

   T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the AtUBI10 promoter.

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

   Inserts

   dCas9-24XGP41
   NbGP41P-sfGFP-VP64-GB1
   

   Use

   Tags

   GB1 and sfGFP

   Expression

   Plant

   Mutation

   Promoter

   AtUBI10

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCI flag PCAF

   Plasmid

   #8941

   Depositor

   Yoshihiro Nakatani

   Article

   Yang et al Nature. 1996 Jul 25. 382(6589):319-24.

   Insert

   PCAF (KAT2B Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. LLP249 pGK-dCas9-DNMT3a V3

   Plasmid

   #100938

   Purpose

   dCas9 and DNMT WT driven by pGK promoter and Puromycin driven by EF1a

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   dCas9, DNMT3a catalytic domain (DNMT3A Human, S.pyogenes)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and 3xTy1

   Expression

   Mammalian

   Mutation

   D10A, H840A for dCas9

   Promoter

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ153

   Plasmid

   #69304

   Purpose

   Gateway entry vector with pco-dCas9-3X(SRDX)

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   pco-dCas9-3X(SRDX) (Plant codon-optimized)
   

   Use

   CRISPR

   Tags

   2XFLAG

   Expression

   Mutation

   SRDX (X3) repressor fusion; NLS fusion

   Promoter

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-ERBB2-V5/HIS

    Plasmid

    #201103

    Purpose

    expression of human ERBB2 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human ERBB2 receptor tyrosine kinase, full length, wildtype (ERBB2 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQTEV-ANP32A

    Plasmid

    #31563

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    acidic (leucine-rich) nuclear phosphoprotein 32 family, member A (ANP32A Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBSDONR P4r-P2

    Plasmid

    #121527

    Purpose

    Gateway Entry Vector for Multisite Gateway Cloning

    Depositor

    Roger Innes

    Article

    Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRRL Luciferase

    Plasmid

    #120798

    Purpose

    For production of lentivirus to express luciferase, primarily for in vivo bioluminescence tracking experiments.

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRAN473

    Plasmid

    #120813

    Purpose

    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target protein

    Depositor

    Craig Ellermeier, David S. Weiss

    Article

    Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1

    Insert

    red fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. retro-gRNA-eGFP

    Plasmid

    #116926

    Purpose

    Retrovirus expressing eGFP with BBSI cloning sites for sgRNA

    Depositor

    Diane Mathis, Christophe Benoist

    Article

    Magnuson et al Proc Natl Acad Sci U S A. 2018 Nov 6;115

    Insert

    eGFP
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. V5-GFP-TurboID-KDEL_pLX208

    Plasmid

    #202020

    Purpose

    expresses GFP-TurboID in the mammalian ER lumen, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    V5-TurboID-KDEL
    

    Use

    Lentiviral

    Tags

    GFP and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSN840

    Plasmid

    #184832

    Purpose

    Expresses human KIF5A(∆exon27) and human KLC1 in insect cells

    Depositor

    Kyoko Chiba

    Article

    Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

    Inserts

    KIF5A (∆exon27) (KIF5A Human)
    KLC1 (KLC1 Human)
    

    Use

    Cre/Lox

    Tags

    His-FLAG and mScarlet-2xStrepII

    Expression

    Insect

    Mutation

    ∆exon27 form of human KIF5A

    Promoter

    p10 and polyhedrin

    Available Since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHB-4

    Plasmid

    #140957

    Purpose

    For PHB production in E. coli (thrC3-pAC). Please note that this is listed as #140956 in the publication.

    Depositor

    Kang Zhou

    Article

    Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

    Insert

    phaCAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+) Flag-His-ATM kd

    Plasmid

    #31986

    Depositor

    Michael Kastan

    Article

    Canman et al Science. 1998 Sep 11;281(5383):1677-9.

    Insert

    ATM kd (ATM Human)
    

    Use

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    3'UTRf 9 nucl/D2870A N2875k. catalytically …

    Promoter

    Available Since

    March 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA-SOCS1

    Plasmid

    #174571

    Purpose

    Human HA-tagged SOCS1 expression (N-term. tag) (CMV prom.)

    Depositor

    Mathew Garnett

    Article

    Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

    Insert

    HA-SOCS1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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