166,033 results

1. pDONR223-TLK2

   Plasmid

   #23629

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   TLK2 (TLK2 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

2. TFORF1842

   Plasmid

   #143324

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   YWHAZ (YWHAZ Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-FLAG-MTOR-I2500F

   Plasmid

   #69014

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-I2500F (MTOR Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Isoleucine 2500 to Phenylalanine

   Available Since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET31b-T7-Spinach2-Ct

   Plasmid

   #79158

   Purpose

   Expresses the Ct biosensor under a T7 promoter

   Depositor

   Ming Hammond

   Article

   Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

   Insert

   T7-tRNA-Spinach2-Ct
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. AP1

   Plasmid

   #179678

   Purpose

   Accessory plasmids

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   [SD8] gIII
   

   Expression

   Bacterial

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-VPR-S-L1-dCjCas9 MiniCAFE

   Plasmid

   #169910

   Purpose

   Expression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.

   Depositor

   Zhili Rong

   Article

   Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

   Insert

   MiniCAFE
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D8A, HNH-truncation (Δ495–609 aa) in CjCas9

   Promoter

   CMV

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. CpxR-GFP

   Plasmid

   #220150

   Purpose

   To track the natural CpxR promoter's transcriptional dynamics

   Depositor

   Andre Sanches Ribeiro

   Article

   Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

   Insert

   CpxR promoter only
   

   Expression

   Bacterial

   Available Since

   Aug. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA GSK3 beta K85A pcDNA3

   Plasmid

   #14755

   Depositor

   Jim Woodgett

   Article

   Hughes et al EMBO J. 1993 Feb . 12(2):803-8.

   Insert

   GSK3 beta K85A (GSK3B Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K85A. Kinase dead mutant.

   Available Since

   April 12, 2007

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. lentiCRISPR v2-Nr4a1 double gRNAs

   Plasmid

   #162791

   Purpose

   Multiplex Guide RNAs to generate Nr4a1 knockout by CRISPR

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Multiplex Nr4a1 gRNA 1 and 2 (Nr4a1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Promoter

   hU6

   Available Since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-Neo-Bam p53 wt

    Plasmid

    #16434

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 (TP53 Human)
    

    Expression

    Mammalian

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. TRE-TDP-43ΔNLS-mClover3

    Plasmid

    #214916

    Purpose

    inducible expression of TDP-43 harboring mutant NLS and C-terminal mClover3. Expression yields cytosolic diffuse TDP-43

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    TDP-43ΔNLS (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    mClover3

    Mutation

    mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKm

    Plasmid

    #170331

    Purpose

    backbone vector for clone construction

    Depositor

    Deqiang Duanmu

    Article

    Wang et al Front Microbiol. 2021 Jun 18;12:686008.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCA14-pHR-SFFV-GFP-RPA43

    Plasmid

    #199445

    Purpose

    Expression of GFP-RPA43 in mammalian cells

    Depositor

    Baohui Chen

    Article

    Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

    Insert

    RPA43 (POLR1F Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. mEmerald-Talin-C-18

    Plasmid

    #62763

    Purpose

    Localization: Talin/Focal Adhesions, C1 Cloning Vector, Excitation:487, Emission:509

    Depositor

    Michael Davidson

    Insert

    Talin
    

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pFLAG-Tetherin

    Plasmid

    #41070

    Depositor

    Paul Spearman

    Article

    Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

    Insert

    Tetherin (BST2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pMXs-mRFP1

    Plasmid

    #21315

    Purpose

    MoMLV-based Retroviral vector, expresses mRFP1

    Depositor

    James Ellis

    Article

    Hotta et al Nat Methods. 2009 May . 6(5):370-6.

    Insert

    monomeric Red Fluorescence Protein 1
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV BiP-Myc-KDEL-wt

    Plasmid

    #27164

    Purpose

    Mammalian expression of human Bip with Myc tag near the C-terminus, retaining the C-terminal KDEL ER localization sequence

    Depositor

    Ron Prywes

    Article

    Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

    Insert

    BiP (HSPA5 Human)
    

    Tags

    KDEL and Myc

    Expression

    Mammalian

    Available Since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.2/v5-DEST hGlut4

    Plasmid

    #18087

    Depositor

    Wolf Frommer

    Article

    Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

    Insert

    Glut4 (SLC2A4 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. mApple-N1

    Plasmid

    #54567

    Purpose

    Localization: N1 Cloning Vector, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Tags

    mApple

    Expression

    Mammalian

    Available Since

    July 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. FUGW-APPIce-T2A-mCherry

    Plasmid

    #190806

    Purpose

    Express Icelandic mutant APP from Ubiquitin C promoter, co-expressing mCherry

    Depositor

    Ronald Hart

    Insert

    APP (APP Human)
    

    Use

    Lentiviral

    Mutation

    Icelandic (A673T)

    Promoter

    Ubi C

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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