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FBP17-pmCherryC1
(Plasmid #27688)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27688 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pmCherry-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4722
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FBP17
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1850
  • Entrez Gene
    FNBP1 (a.k.a. FBP17)
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer mCherry-F 5'-ccccgtaatgcagaagaaga
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Cloned from cDNA library from human kidney.

This plasmid may be slow growing in bacteria. For difficult constructs you can use TG1s (they are Endonuclease positive, but good for cloning) and INV F' E.coli strains. The latter give the best results. Also when growing in culture try to switch to 2xTY media, which is richer that LB.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FBP17-pmCherryC1 was a gift from Christien Merrifield (Addgene plasmid # 27688 ; http://n2t.net/addgene:27688 ; RRID:Addgene_27688)
  • For your References section:

    A high precision survey of the molecular dynamics of Mammalian clathrin-mediated endocytosis. Taylor MJ, Perrais D, Merrifield CJ. PLoS Biol. 2011 Mar . 9(3):e1000604. 10.1371/journal.pbio.1000604 PubMed 21445324