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TrHKII-pGFPN3
(Plasmid #21921)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21921 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGFP-N3
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha, HB101, and other general purpose strains.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Truncated human HKII cDNA (lacking the sequence encoded by exon 1, which is the mitochondrial binding domain) in pGFP-N3 plasmid
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2751
  • Mutation
    This is the truncated human HKII cDNA sequence (lacking exon 1 whoch encoded the mitochondrial binding domain) cloned into pGFP-N3.
  • GenBank ID
    NM_000189
  • Entrez Gene
    HK2 (a.k.a. HKII, HXK2)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer NA
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TrHKII-pGFPN3 was a gift from Hossein Ardehali (Addgene plasmid # 21921 ; http://n2t.net/addgene:21921 ; RRID:Addgene_21921)
  • For your References section:

    Glucose phosphorylation and mitochondrial binding are required for the protective effects of hexokinases I and II. Sun L, Shukair S, Naik TJ, Moazed F, Ardehali H. Mol Cell Biol. 2008 Feb . 28(3):1007-17. 10.1128/MCB.00224-07 PubMed 18039843