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TrHKI-pGFPN3
(Plasmid #21918)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21918 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 21918-D50 50 μg of DNA in Tris buffer $495
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DNA 21918-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pGF-N3
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha, HB101 and other general purpose strains
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Truncated human HKI (lacking exon 1 sequence that encodes for the mitochondrial binding domain) in pGFP-N3 plasmid
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2688
  • Mutation
    This is the truncated human HKI cDNA (lacking exon 1 that encodes for the mitochondrial binding domain of HKI) in pGFP-N3
  • GenBank ID
    M75126
  • Entrez Gene
    HK1 (a.k.a. HK, HK1-ta, HK1-tb, HK1-tc, HKD, HKI, HMSNR, HXK1, NEDVIBA, NMSR, RP79, hexokinase)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TrHKI-pGFPN3 was a gift from Hossein Ardehali (Addgene plasmid # 21918 ; http://n2t.net/addgene:21918 ; RRID:Addgene_21918)

  • For your References section:

    Glucose phosphorylation and mitochondrial binding are required for the protective effects of hexokinases I and II. Sun L, Shukair S, Naik TJ, Moazed F, Ardehali H. Mol Cell Biol. 2008 Feb . 28(3):1007-17. 10.1128/MCB.00224-07 PubMed 18039843
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