pACAGW-H2B-PAGFP-AAV
(Plasmid #33000)

• Depositing Lab: Massimo Scanziani
• Publication: Lien et al Front Neural Circuits. 2011;5:16. Epub 2011 Nov 29.

Backbone

• Vector backbone: N/A
• Backbone size w/o insert (bp): 4771
• Vector type: Mammalian Expression, AAV ; adeno associated virus

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl2
• Growth instructions: Grow on Stbl2 cells
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: H2B-PAGFP
• Alt name: histone2b-photoactivatable gfp
• Species: H. sapiens (human); Aequorea victoria
• Insert Size (bp): 1116
• Entrez Gene: H2BC21 (a.k.a. GL105, H2B, H2B-GL105, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE)
• Tag / Fusion Protein:
  • PAGFP (C terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: André Nussenzweig
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid containing the H2B-PAGFP fusion gene was a gift from André Nussenzweig (Kruhlak et al., 2006). The H2B-PAGFP gene was subcloned into the pACAGW-ChR2-Venus-AAV plasmid (Addgene # 20071).

Kruhlak, M. J., Celeste, A., Dellaire, G., Fernandez-Capetillo, O., Müller, W. G., McNally, J. G., Bazett-Jones, D. P., and Nussenzweig, A. (2006). Changes in chromatin structure and mobility in living cells at sites of DNA double-strand breaks. J. Cell Biol. 172, 823-34.

There is a 5bp deletion in Addgene's sequence compared to depositor's reference sequence. The deletion is in the vector sequence upstream of the ORF and does not affect plasmid function. There is also a mutation D26G in H2B; this mutation is found in depositor's reference sequence as well.