Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||44161||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 7200
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Alt nameTransforming growth factor-beta-activated kinase
SpeciesM. musculus (mouse)
Insert Size (bp)1800
Entrez GeneMap3k7 (a.k.a. B430101B05, Tak1)
/ Fusion Protein
- Flag (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Article Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Alternative plasmid name: pcDNA Flag-TAK1
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3 TAK1/F was a gift from Xin Lin (Addgene plasmid # 44161 ; http://n2t.net/addgene:44161 ; RRID:Addgene_44161)
For your References section:TAK1 is recruited to the tumor necrosis factor-alpha (TNF-alpha) receptor 1 complex in a receptor-interacting protein (RIP)-dependent manner and cooperates with MEKK3 leading to NF-kappaB activation. Blonska M, Shambharkar PB, Kobayashi M, Zhang D, Sakurai H, Su B, Lin X. J Biol Chem. 2005 Dec 30;280(52):43056-63. Epub 2005 Oct 31. 10.1074/jbc.M507807200 PubMed 16260783