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pLenti6.3-hEPO
(Plasmid #50436)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50436 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLenti6.3/V5-DEST gateway vector
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 9387
  • Total vector size (bp) 10000
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EPO
  • Alt name
    erythropoietin
  • Alt name
    MVCD2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    582
  • Entrez Gene
    EPO (a.k.a. DBAL, ECYT5, EP, MVCD2)
  • Promoter CMV

Cloning Information

Resource Information

Depositor Comments

Human erythropoietin (hEPO) ORF with stop codon (582 bp; Ultimate ORF clone number IOH7334) from GeneCopoeia ORF cDNA clones in pDONR vector (GeneCopoeia; GC-A1011).
Because the stop codon is inserted before V5 epitope, the V5 tag on the pLenti6.3/V5-DEST vector won’t be transcribed.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti6.3-hEPO was a gift from Juan Melero-Martin (Addgene plasmid # 50436)
  • For your References section:

    Induction of erythropoiesis using human vascular networks genetically engineered for controlled erythropoietin release. Lin RZ, Dreyzin A, Aamodt K, Li D, Jaminet SC, Dudley AC, Melero-Martin JM. Blood. 2011 Nov 17;118(20):5420-8. doi: 10.1182/blood-2011-08-372946. Epub 2011 Sep 21. 10.1182/blood-2011-08-372946 PubMed 21937702