-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 10954 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepSV2neo
-
Backbone manufacturerATCC
- Backbone size w/o insert (bp) 5500
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameinterferon-gamma
-
Alt nameIFN-gamma
-
SpeciesH. sapiens (human)
-
Insert Size (bp)8600
-
Entrez GeneIFNG (a.k.a. IFG, IFI, IMD69)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer AGT AAC AGC TTC CCA CAT CA
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Human genomic DNA containing the IFN-gamma gene, approximately 2.7 kb upstream and 1 kb downstream of exons, poly A site. Young lab stock #D3126.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pSV2neo human IFN-gamma was a gift from Howard Young (Addgene plasmid # 10954 ; http://n2t.net/addgene:10954 ; RRID:Addgene_10954) -
For your References section:
Expression of transfected human interferon-gamma DNA: evidence for cell-specific regulation. Young HA, Dray JF, Farrar WL. J Immunol. 1986 Jun 15. 136(12):4700-3. PubMed 3011901