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Elimination of paternal mitochondria in mouse embryos occurs through autophagic degradation dependent on PARKIN and MUL1.
Rojansky R, Cha MY, Chan DC
Elife. 2016 Nov 17;5. pii: e17896. doi: 10.7554/eLife.17896.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
78518pBABEpuro mTurquoise2 LC3BFluorescent reporter for LC3B visualization
78520pCLBW cox8 EGFP mCherryFluorescent mitophagy reporter
78522pFUChW nontargeting shRNALentiviral vector encoding shRNA and mCherry reporter
78523pFUChW Parkin shRNA Mul1 shRNALentiviral vector encoding two shRNAs and mCherry reporter