PurposeFluorescent protein for superresolution imaging and a focal adhesion protein for binding substrate
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||101151||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Gene/Insert namehuman codon optimized mMaple3 fused to zyxin
SpeciesH. sapiens (human), Synthetic
- Promoter cmv
- Cloning method Gibson Cloning
- 5′ sequencing primer CTGCTCTGAGCCCATCATGC
- 3′ sequencing primer GGACAAACCACAACTAGAATGC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pZyxin-mMaple3 was a gift from Xiaowei Zhuang (Addgene plasmid # 101151 ; http://n2t.net/addgene:101151 ; RRID:Addgene_101151)
For your References section:Characterization and development of photoactivatable fluorescent proteins for single-molecule-based superresolution imaging. Wang S, Moffitt JR, Dempsey GT, Xie XS, Zhuang X. Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):8452-7. doi: 10.1073/pnas.1406593111. Epub 2014 May 27. 10.1073/pnas.1406593111 PubMed 24912163