166,289 results

1. NG-ABE9

   Plasmid

   #240625

   Purpose

   Adenine base editor with narrowed editing window, recognizing NG PAM

   Depositor

   Tingting Jiang

   Article

   Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:

   Insert

   TadA8e (N108Q/L145T)-linker-nCas9 (D10A, L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   TadA8e (N108Q/L145T), Cas9 (D10A, L1111R, D1135V,…

   Available Since

   July 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. PET-B-HypaSpCas9-NLS-6xHis

   Plasmid

   #207386

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HypaSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N692A, M694A, Q695A, H698A, amino acids 1005-1013…

   Promoter

   T7

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHage2-EF1a-mCherryTRF1-IRES-Blast

   Plasmid

   #182588

   Purpose

   expresses mcherry-trf1 in mammalian cells

   Depositor

   Agnel Sfeir

   Article

   Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

   Insert

   hTRF1 (TERF1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-OptoTGFBRs

   Plasmid

   #118942

   Purpose

   Mammalian expression plasmid of OptoTGFBRs (optogenetically-activated TGFb receptors)

   Depositor

   Won Do Heo

   Article

   Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

   Insert

   OptoTGFBRs (Tgfb1, Tgfbr2 Mustard Weed, Mouse)
   

   Tags

   tdTomato

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. elavl3:hArr-TEV;he1.1:YFP

   Plasmid

   #218855

   Purpose

   D. rerio elavl3 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.

   Depositor

   Marnie Halpern

   Article

   Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

   Insert

   hArr
   

   Tags

   human Arrestin-Tobbaco Etch Virus protease fusion…

   Expression

   Bacterial

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZVsE_SC12

   Plasmid

   #219722

   Purpose

   Zika virus stable E dimer with fusion loop mutation and increased expression

   Depositor

   Brian Kuhlman

   Article

   Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi

   Insert

   ZKV sE SC12
   

   Tags

   8xHis and human serum albumin (HSA)

   Expression

   Mammalian

   Mutation

   G106D, A264W, T267R, S286W, S287P, G29K, A25M

   Available Since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. His6_MBP_FMRP_P451S

   Plasmid

   #198693

   Purpose

   Expression of human FMRP protein in E. coli

   Depositor

   Simpson Joseph

   Article

   Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

   Insert

   FMR1 (FMR1 Human)
   

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   changed proline 451 to serine

   Promoter

   T7

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. mPapaya1-H2B-6

   Plasmid

   #56651

   Purpose

   Localization: Nucleus/Histones, Excitation: 530, Emission: 541

   Depositor

   Michael Davidson

   Article

   Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

   Insert

   H2B (H2BC11 Human)
   

   Tags

   mPapaya1

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available Since

   Dec. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVSVΔG-eGFP-linker

   Plasmid

   #231707

   Purpose

   Encodes the genome of VSV driven by a T7 polymerase promoter. It lacks the G protein (glycoprotein) and encodes eGFP.

   Depositor

   Ron Geller

   Article

   Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1

   Insert

   VSV antigenome
   

   Use

   Vsv expression

   Expression

   Mammalian

   Mutation

   ΔG (VSV glycoprotein removed)

   Promoter

   T7

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. mEGFP-HRas G12V

    Plasmid

    #18666

    Depositor

    Karel Svoboda

    Article

    Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

    Insert

    H-Ras (HRAS Human)
    

    Tags

    monomeric EGFP

    Expression

    Mammalian

    Mutation

    G12V mutation in HRas

    Available Since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-BirA*-dCas9-eGFP

    Plasmid

    #84779

    Purpose

    Expresses BirA*-dCas9-eGFP in mammalian cells.

    Depositor

    Heinrich Leonhardt

    Article

    Schmidtmann et al Nucleus. 2016 Sep 27:0.

    Insert

    BirA*-dCas9-eGFP
    

    Use

    CRISPR

    Tags

    cMyc

    Expression

    Bacterial and Mammalian

    Promoter

    CAG

    Available Since

    July 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBABE puro human IRS-1

    Plasmid

    #11025

    Depositor

    Ronald Kahn

    Article

    Bruning et al Mol Cell Biol. 1997 Mar . 17(3):1513-21.

    Insert

    IRS-1 (IRS1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDSW1728

    Plasmid

    #120812

    Purpose

    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt)

    Depositor

    Craig Ellermeier, David S. Weiss

    Article

    Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1

    Insert

    red fluorescent protein (mCherry), codon-optimized for C. difficile
    

    Expression

    Bacterial

    Promoter

    Ptet

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAS9c

    Plasmid

    #141250

    Purpose

    Constitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    TEF1 (Cas9), pSNR52 (gRNA)

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT22-GluCNZ-6His

    Plasmid

    #213504

    Purpose

    Designed for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.

    Depositor

    John Denu

    Article

    Kuznetsov et al J Biol Chem. 2018 Aug 3;293(31):12271-12

    Insert

    Glucanase (glucan endo-1,3-beta-glucosidase)
    

    Tags

    6His tag

    Expression

    Bacterial

    Mutation

    1-36 Signal Sequence Deletion

    Promoter

    T7

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. 765 pME nls mCerulean

    Plasmid

    #195958

    Purpose

    Gateway pME fluorophore

    Depositor

    Kristen Kwan, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    mCerulean nuclear
    

    Use

    Other

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3 c-SRC (WT)

    Plasmid

    #42202

    Depositor

    Robert Lefkowitz

    Article

    Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

    Insert

    c-SRC (SRC Human)
    

    Expression

    Mammalian

    Mutation

    L176F mutation compared to reference sequence NM_…

    Promoter

    CMV

    Available Since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBBR1_DVK_AE(J23100-BCD2-E0040m-B0015)

    Plasmid

    #197660

    Purpose

    Expresses GFP under the control of J23100 and BCD2

    Depositor

    Eric Young

    Article

    Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223-NME6

    Plasmid

    #23562

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    NME6 (NME6 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pX330A-FBL/PITCh

    Plasmid

    #63671

    Purpose

    Expresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only