A CRISPR system permits genetic engineering of essential genes and gene families.
Vyas VK, Barrasa MI, Fink GR
Sci Adv. 2015;1(3):e1500248. PubMed Article
Vyas VK, Barrasa MI, Fink GR
Sci Adv. 2015;1(3):e1500248. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 111423 | pV1010 | gRNA plasmid for cleavage of Candida albicans ADE2 - integrates at RPS1 (Part of Duet system) |
| 111424 | pV1025 | Expression of Candida albicans codon-optimized Cas9 (CaCas9) - integrates at ENO1 (Part of Duet system) |
| 111425 | pV1081 | Solo vector for expression of CaCas9 and sgRNA for CaADE2 mutagenesis - integrates at ENO1 |
| 111427 | pV1090 | gRNA entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at RPS1 (Part of Duet system) |
| 111428 | pV1093 | CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 |
| 111429 | pV1200 | CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP) |