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A CRISPR system permits genetic engineering of essential genes and gene families.
Vyas VK, Barrasa MI, Fink GR
Sci Adv. 2015;1(3):e1500248.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
111423pV1010gRNA plasmid for cleavage of Candida albicans ADE2 - integrates at RPS1 (Part of Duet system)
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111424pV1025Expression of Candida albicans codon-optimized Cas9 (CaCas9) - integrates at ENO1 (Part of Duet system)
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111425pV1081Solo vector for expression of CaCas9 and sgRNA for CaADE2 mutagenesis - integrates at ENO1
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111427pV1090gRNA entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at RPS1 (Part of Duet system)
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111428pV1093CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1
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111429pV1200CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP)
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