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PL-SIN-EOS-S(4+)-EGFP

Plasmid

#21317

Purpose

Lentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EOS-S(4+) promoter region

Available since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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pROP-IP-GFP

Plasmid

#122136

Purpose

GFP Reporter plasmid for B-Rex contain imperfect operator sequence

Depositor

Srivatsan Raman

Article

Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23101

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-FGFR1

Plasmid

#116740

Purpose

Lentiviral expression of FGFR1

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FGFR1 (FGFR1 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Luc-PS9

Plasmid

#177942

Purpose

Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

Luc2 (ORF1ab )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX/PD-L1 WT

Plasmid

#121466

Purpose

Expression of GST-human PD-L1 in E. Coli

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA

Plasmid

#108871

Purpose

Expression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafish

Depositor

Alex Schier

Article

Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

Inserts

5xU6:sgRNA
Cas9-t2A-GFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6 and hsp70

Available since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-nef-CIAO2-Flp

Plasmid

#149296

Purpose

Cre-dependent expression of Flp recombinase.

Depositor

Edward Callaway

Article

Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

Insert

Flp recombinase (flp Budding Yeast)

Use

AAV

Tags

Expression

Mutation

Derived from the mouse codon-optomized FlpO. The…

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ 2xFLAG-2xSTREP_BACH1

Plasmid

#159130

Purpose

expresses BACH1 in mammalian cells

Depositor

Michele Pagano

Article

Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

Insert

BACH1 (BACH1 Human)

Use

Tags

2X-FLAG _ 2X-STREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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GST-RH▲-MNase

Plasmid

#136292

Purpose

Expression of catalytic inactive RNase H fused to micrococcal nuclease

Depositor

Kavitha Sarma

Article

Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

Insert

RNase HI-D10R-E48R and MNase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBbS5a-Opto-Cre-Vvd-2

Plasmid

#160400

Purpose

Updated light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.

Depositor

Mary Dunlop

Article

Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

Insert

Opto-Cre-Vvd-2

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

lacUV5

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pFPV25.1

Plasmid

#20668

Purpose

S. typhimurium rpsM promoter driving gfpmut3 expression in bacteria. Promoter is not regulated by acidic conditions.

Depositor

Raphael Valdivia

Article

Valdivia et al Mol Microbiol. 1996 Oct . 22(2):367-78.

Type

Empty backbone

Use

Tags

GFP mut3

Expression

Bacterial

Mutation

Promoter

Available since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC7A5_STOP

Plasmid

#161363

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC7A5 (SLC7A5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CS-TRE-c-Myc-Ubc-rtTA

Plasmid

#198190

Purpose

Generation of Expandable megakaryocyte cell lines from human iPS cells

Depositor

Hiromitsu Nakauchi

Article

Nakamura et al Cell Stem Cell. 2014 Apr 3;14(4):535-48.

Insert

hC-MYC (MYC Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_BiCHATe27_Gq_dTomato

Plasmid

#213832

Purpose

AAV construct expressing HA-hM3Dq-dTomato driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_Gq_dTomato

Depositor

Gordon Fishell

Article

Furlanis et al bioRxiv 2024.07.17.603924

Insert

HA-GqDREADD-dTomato

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag beta-2-adrenergic-receptor

Plasmid

#14697

Depositor

Robert Lefkowitz

Article

Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

Insert

B2AR (ADRB2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1

Plasmid

#215030

Purpose

Mammalian expression of human TWIST1

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-24p3/LCN2-Luc

Plasmid

#25463

Depositor

Sarah Gaffen

Article

Shen et al J Leukoc Biol. 2005 Mar . 77(3):388-99.

Insert

24p3/lipocalin 2 proximal promoter (Lcn2 Mouse)

Use

Luciferase

Tags

Expression

Mutation

none

Promoter

Available since

June 25, 2010

Availability

Academic Institutions and Nonprofits only
Shank3-HA/pRK5

Plasmid

#197335

Purpose

Plasmid expressing an antigen targeting Shank3

Depositor

Morgan Sheng

Insert

Shank3 (Shank3 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-RhoA-T19N

Plasmid

#12967

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

RhoA DN (RHOA Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T19N Dominant negative

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pRSET-QUE2m

Plasmid

#129350

Purpose

ATP measurement

Depositor

Hiroyuki Noji

Article

Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

Insert

QUEEN-2m

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 25, 2019

Availability

Academic Institutions and Nonprofits only

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