164,722 results

1. pDD120

   Plasmid

   #119879

   Purpose

   Constitutive expression of Che9c60 and Che9c61 recombinases on pB264/pBR322 backbone with kanamycin resistance.

   Depositor

   Tae Seok Moon

   Article

   DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

   Insert

   pConstitutive_Che9c60 and Che9c61
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. LifeAct-EGFP-APEX2 in pCSDEST2

   Plasmid

   #170523

   Purpose

   Vector for expression of LifeAct APEX2 in mammalian cells. Parton lab clone FTE

   Depositor

   Rob Parton

   Article

   Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

   Insert

   Cavin4 (Cavin4 Mouse)
   

   Use

   Tags

   APEX2-P2A-mKate2

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKI_ΩBBMVP16&SRDXWUSm1

   Plasmid

   #220349

   Purpose

   Expresses BBM-VP16 and SRDX-WUSm1 in plant cells

   Depositor

   Tomoko Igawa

   Article

   Sato et al Front Plant Sci. 2024 Apr 3;15:1308417.

   Inserts

   BABY BOOM (BBM Mustard Weed)
   WUSCHEL (WUS Mustard Weed)
   

   Use

   Tags

   Superman Repression Domain X (SRDX) and VP16 tran…

   Expression

   Plant

   Mutation

   two amino acid substitution in WUS-box: L255A L25…

   Promoter

   CaMV 35S and RPS5A

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOPINK-OTUD4 (OTU, aa 22-196)

   Plasmid

   #61413

   Purpose

   Expresses human OTUD4 (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD4 (OTU domain-containing protein 4) (OTUD4 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-21 and aa 197-1114.

   Promoter

   T7

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLumi-LgBiT

   Plasmid

   #188319

   Purpose

   Expression of LgBiT via sigma 70 promoter in E. coli

   Depositor

   Kate Adamala

   Article

   Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

   Insert

   LgBiT
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   sigma 70 promoter

   Available Since

   Sept. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. CAG-Cas9-T2A-EGFP-ires-puro

   Plasmid

   #78311

   Purpose

   Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

   Depositor

   Timo Otonkoski

   Article

   Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

   Insert

   WT SpCas9-T2A-EGFP-ires-puromycin resistance
   

   Use

   CRISPR

   Tags

   T2A-EGFP-ires-puro

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCerulean-Nucleus-12

   Plasmid

   #54556

   Purpose

   Localization: Nucleus, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   mCerulean-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

8. Str-KDEL_ST-SBP-EGFP

   Plasmid

   #65264

   Purpose

   synchronize trafficking of ST from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
   

   Use

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. Col1a-frt-hygro-pA

   Plasmid

   #20730

   Depositor

   Rudolf Jaenisch

   Article

   Beard et al Genesis. 2006 Jan . 44(1):23-8.

   Insert

   Col1A-frt-hygro-pA
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 24, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-EFS-NLS-CasRx-U6-DR-sgRNA_LacZ-DR

    Plasmid

    #154003

    Purpose

    Vector expressed CasRx and sgRNA_LACZ for AAV package

    Depositor

    Hui Yang

    Article

    He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

    Insert

    CasRx
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available Since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNatB (pACYCduet-naa20-naa25)

    Plasmid

    #53613

    Purpose

    Allows expression of the fission yeast NatB complex - chloramphenicol marker

    Depositor

    Dan Mulvihill

    Article

    Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

    Inserts

    naa20 (naa20 Fission Yeast)
    naa25 (naa25 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBABEpuro-ERBB2

    Plasmid

    #40978

    Depositor

    Matthew Meyerson

    Article

    Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF0266

    Plasmid

    #142578

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TCF4 (TCF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. PGK-H2BeGFP

    Plasmid

    #21210

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    Phosphoglycerate kinase promoter (PGK1 Human)
    

    Use

    Lentiviral

    Tags

    H2B-eGFP

    Expression

    Mutation

    Promoter

    Available Since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEF.DEST51-mVenus

    Plasmid

    #154899

    Purpose

    Expresses a fluorescent protein, mVenus

    Depositor

    Johanna Ivaska

    Article

    Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

    Insert

    mVenus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Flag-Keap1

    Plasmid

    #28023

    Depositor

    Qing Zhong

    Article

    Fan et al Autophagy. 2010 Jul 22. 6(5):.

    Insert

    Keap1 (KEAP1 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAVS1-Bxb1-LP-TC

    Plasmid

    #183754

    Purpose

    AAVS1 targeting construct with the Bxb1 landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    loxP-eGFP-attP-Bsd-lox257
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMK289 (mAID-mClover-NeoR)

    Plasmid

    #72827

    Purpose

    C-terminal tagging with mAID-mClover using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-mClover-Neo
    

    Use

    CRISPR

    Tags

    mAID-mClover

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. NanoLuc-PM

    Plasmid

    #164784

    Purpose

    Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors

    Depositor

    Laszlo Hunyady

    Article

    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

    Insert

    Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Oplophorus gracilirostris, Human)
    

    Use

    Tags

    NanoLuc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330 Pten

    Plasmid

    #59909

    Purpose

    pX330 backbone expressing sgRNA targeting Pten to edit mouse Pten. Expresses Cas9 from CBh promoter

    Depositor

    Tyler Jacks

    Article

    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

    Insert

    sgRNA targeting Pten (Pten Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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