166,289 results

1. PX459v3

   Plasmid

   #178799

   Purpose

   SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Cbh (Cas9-2A Puro) and U6 (gRNA)

   Available Since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX-EF1a_Empty

   Plasmid

   #221480

   Purpose

   Empty vector control for pLX307 overexpresion experiments

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag-ZDHHC21

   Plasmid

   #231669

   Purpose

   Expresses human ZDHHC21protein

   Depositor

   Chunhong Ma

   Article

   Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

   Insert

   ZDHHC21 (ZDHHC21 Human)
   

   Tags

   3×Flag Tag

   Expression

   Mammalian

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. EF1a-mito-dsRED2

   Plasmid

   #174541

   Purpose

   mitochondria targeted dsRED expressed off of EF1a promoter

   Depositor

   Thomas Schwarz

   Insert

   Mito
   

   Use

   Lentiviral

   Tags

   dsRED

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEAK10-His-Myc-Kal9

   Plasmid

   #25441

   Depositor

   Betty Eipper

   Article

   Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

   Insert

   Kal9
   

   Tags

   His and Myc

   Expression

   Mammalian

   Available Since

   July 15, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pFCIV-CEACAM6

   Plasmid

   #149303

   Purpose

   lentiviral packaging vector for CEACAM6 expression.

   Depositor

   James Fleckenstein

   Article

   Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

   Insert

   CEACAM6 (CEACAM6 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   ubiquitin C

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHyo094 (eMutaT7)

   Plasmid

   #173124

   Purpose

   Expresses eMutaT7 for mutagenizing genes downstream from a T7 promoter in e.coli

   Depositor

   Seokhee Kim

   Article

   Park et al Nucleic Acids Res. 2021 Apr 6;49(6):e32.

   Insert

   pmCDA1-T7RNAP
   

   Expression

   Bacterial

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-AXL-V5/HIS

   Plasmid

   #200117

   Purpose

   Expression of human AXL receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human AXL receptor tyrosine kinase (AXL Human)
   

   Tags

   V5/His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. EndophilinA1-mRuby2

   Plasmid

   #171945

   Purpose

   Expresses Endophilin A1 in mammalian cells

   Depositor

   Cheng-han Yu

   Article

   Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   Endophilin A1 (SH3GL2 Human)
   

   Tags

   mRuby2

   Expression

   Mammalian

   Available Since

   July 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEAK10-His-Myc-Kal5

    Plasmid

    #25440

    Depositor

    Betty Eipper

    Article

    Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

    Insert

    Kal5
    

    Tags

    His and Myc

    Expression

    Mammalian

    Available Since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pT3EF1aH-myr-Akt

    Plasmid

    #179909

    Purpose

    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.

    Depositor

    Xin Chen

    Article

    Ho et al Hepatology. 2012 Mar;55(3):833-45. doi:

    Insert

    Akt1 (Akt1 Mouse)
    

    Tags

    HA and Myr

    Expression

    Mammalian

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDF0112 cmv-cluciferase-polya-ef1a-g-luciferase-polya

    Plasmid

    #186980

    Purpose

    mammalian expression of Cluc-Gluc reporter

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

    Insert

    Cluc, Gluc
    

    Use

    Luciferase

    Available Since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-Casp2-Flag

    Plasmid

    #11811

    Depositor

    Guy Salvesen

    Insert

    Caspase-2 (CASP2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-CMV-Galpha-s ONE-GO

    Plasmid

    #189731

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-s ONE-GO
    GNAS (GNAS Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pLPT41

    Plasmid

    #85524

    Purpose

    Titration sponge for TetR

    Depositor

    Johan Paulsson

    Article

    Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

    Type

    Empty backbone

    Use

    Synthetic Biology

    Promoter

    PLtet

    Available Since

    April 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL3-Basic-Rab18-sfGFP(N) HDR template

    Plasmid

    #129415

    Purpose

    HDR tempalte for tagging of endogenous human RAB18 N-terminus with sfGFP

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    RAB18 HDR template (RAB18 Human)
    

    Use

    CRISPR and TALEN; Endogenous tagging hdr template

    Tags

    sfGFP

    Expression

    Mammalian

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOpen-EcoRIR

    Plasmid

    #165504

    Purpose

    Type II restriction enzyme that recognizes the double-stranded sequence GAATTC and cleaves after G-1.

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    EcoRI
    

    Use

    Synthetic Biology

    Available Since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. lenti-sgRNA neo

    Plasmid

    #104992

    Purpose

    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and G418 (neomycin) resistance from an EF-1a promoter.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV_Zika_NS5_Flag

    Plasmid

    #79639

    Purpose

    Third generation Self-inactivating lentivector expressing NS5 from Zika virus

    Depositor

    Vaithi Arumugaswami

    Insert

    NS5
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC57 (Gent)

    Plasmid

    #54338

    Depositor

    Martin Parniske

    Article

    Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

    Type

    Empty backbone

    Use

    Cloning vector

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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