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PurposeECFP-EYFP FRET positive control
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 109330 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepECFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4731
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameECFP-EYFP FRET positive control
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SpeciesSynthetic
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Insert Size (bp)773
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MutationECFP linked to EYFP with 18 aa linker GLRSRAQASNSAVEGSAM
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV_F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pECFP18aaEYFP was a gift from Gabriele Kaminski Schierle (Addgene plasmid # 109330 ; http://n2t.net/addgene:109330 ; RRID:Addgene_109330) -
For your References section:
A quantitative protocol for intensity-based live cell FRET imaging. Kaminski CF, Rees EJ, Schierle GS. Methods Mol Biol. 2014;1076:445-54. doi: 10.1007/978-1-62703-649-8_19. 10.1007/978-1-62703-649-8_19 PubMed 24108638
