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Plasmid#51310PurposeExpresses far-red fluorescent protein mNeptune2.5 in mammalian cellsDepositorInsertmNeptune2.5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDLxR6
Plasmid#172560PurposeInducible gene expression, pCDF origin, LuxR activator, PLuxB promoter, mRFP, Streptomycin Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLuxBAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IB-HaloTag7-mGFP-KDEL
Plasmid#184904PurposeStable expression of HaloTag7-mGFP-KDEL in mammalian cells to measure ER-phagy activityDepositorInsertKDEL
UseTagsHaloTag7 and monomeric EGFPExpressionMammalianMutationPromoterAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHA2
Plasmid#85710PurposeThis plasmid provides a BAC vector backbone containing GFP, which can be used for cloning of BAC inserts, for instance, herpesvirus genomesDepositorTypeEmpty backboneUseCre/Lox; Herpesvirus cloningTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Caprola_01-mEGFP
Plasmid#194681PurposeCMV driven expression of the calcium recorder Caprola_01 in mammalian cell lines fused to mEGFPDepositorInsertCaprola_01-mEGFP
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-Ef1a-Puro
Plasmid#227266PurposeEmpty AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-myc3-CUL2
Plasmid#19892DepositorInsertcullin 2 (CUL2 Human)
UseTagsmyc3ExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGL4.R_TRE_minCMV
Plasmid#211518PurposeDual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minCMV-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterSV40 and minCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG HRP-TM
Plasmid#44441DepositorArticleInsertHorseradish peroxidase fused to the transmembrane domain of PDGFR
UseTagsHA tag and myc tagExpressionMammalianMutationPromoterCAGAvailable sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC25A39
Plasmid#131970PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC25A39 (SLC25A39 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti-hSyn-nucGFP
Plasmid#140190PurposeOverexpression of GFP with a nuclear localization sequence, under the human synapsin promoterDepositorInsertGFP-NLS
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pGL003
Plasmid#215335PurposeamyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank promoter)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterpSpankAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PalmGRET
Plasmid#158221PurposeBRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.DepositorInsertPalmGFP-Nluc
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
MyrPalm-CKAR
Plasmid#14862DepositorInsertC kinase activity reporter
UseTagsCFP, Myr/Palm, and YFPExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10/RSV_SF3B3-miniXon_Luciferase
Plasmid#174660PurposePlasmid containing the SF3B3-miniXon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-miniXon by LMI070.DepositorInsertminiXon cassette
UseLuciferaseTagsExpressionMammalianMutationKozak ATG initiation sequence in middle exonPromoterRSVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
XRE-H2B-eGFP
Plasmid#182294PurposeFluorescent reporter for AhR activityDepositorInsertXenobiotic Response Element (CYP1A1 Human)
UseTagseGFPExpressionMammalianMutationPromoterXREAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICH86966::AtU6p::sgRNA_PDS
Plasmid#46966PurposeExpresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoterDepositorInsertAtU6p::sgRNA_PDS
UseCRISPR; Plant expressionTagsExpressionMutationPromoterAvailable sinceAug. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-DRGFP
Plasmid#113193PurposeDR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.DepositorInsertDR-GFP
UseDonor construct with t2a-hygromycin for gene trap…TagsExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only