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Addgene

pRH030
(Plasmid #159633)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159633 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJOE8999
  • Vector type
    CRISPR ; shuttle vector
  • Promoter Constitutive tracrRNA

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Bacillus subtilis
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRH030 was a gift from Robert Hertel (Addgene plasmid # 159633 ; http://n2t.net/addgene:159633 ; RRID:Addgene_159633)

  • For your References section:

    A CRISPR-Cas9 tool to explore the genetics of Bacillus subtilis phages. Otte K, Kuhne NM, Furrer AD, Baena Lozada LP, Lutz VT, Schilling T, Hertel R. Lett Appl Microbiol. 2020 Jul 2. doi: 10.1111/lam.13349. 10.1111/lam.13349 PubMed 32615024
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