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pRSET FlipSuc-90u delta1
(Plasmid #14475)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14475 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FlipThuE W283A
  • Alt name
    ECFP-sucBP-EYFP
  • Alt name
    Sucrose binding protein
  • Alt name
    AtThuE
  • Species
    Agrobacterium tumefaciens
  • Insert Size (bp)
    2800
  • Mutation
    W283A, 13 amino acids deleted from linkers
  • Tags / Fusion Proteins
    • ECFP (N terminal on insert)
    • EYFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

For more information, see
http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html

FLIPsuc-90u{Delta}1 was created by sub-cloning AtThuE into FLIPglu-600µ{Delta}5 with KpnI, which created a construct with 13 amino acids deleted from the linkers.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSET FlipSuc-90u delta1 was a gift from Wolf Frommer (Addgene plasmid # 14475)
  • For your References section:

    Conversion of a putative Agrobacterium sugar-binding protein into a FRET sensor with high selectivity for sucrose. Lager I, Looger LL, Hilpert M, Lalonde S, Frommer WB. J Biol Chem. 2006 Oct 13. 281(41):30875-83. 10.1074/jbc.M605257200 PubMed 16912038