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Addgene

pRSET FlipSuc-90u
(Plasmid #13558)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13558 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRSET-B
  • Backbone size w/o insert (bp) 2897
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FlipThuE W283A
  • Alt name
    ECFP-sucBP-EYFP
  • Alt name
    Sucrose binding protein
  • Alt name
    AtThuE
  • Species
    Agrobacterium tumefaciens
  • Insert Size (bp)
    2400
  • Mutation
    W283A in binding protein
  • Tags / Fusion Proteins
    • ECFP (N terminal on insert)
    • EYFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSET FlipSuc-90u was a gift from Wolf Frommer (Addgene plasmid # 13558 ; http://n2t.net/addgene:13558 ; RRID:Addgene_13558)
  • For your References section:

    Conversion of a putative Agrobacterium sugar-binding protein into a FRET sensor with high selectivity for sucrose. Lager I, Looger LL, Hilpert M, Lalonde S, Frommer WB. J Biol Chem. 2006 Oct 13. 281(41):30875-83. 10.1074/jbc.M605257200 PubMed 16912038