We narrowed to 13,507 results for: GAN

1. pBABE-2xFLAG-2xSTREP-CCND1-Puro

   Plasmid

   #172634

   Purpose

   Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D1 and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Retroviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-2xFLAG-2xSTREP-CCND1(P287L)-IRES-mCherry

   Plasmid

   #172636

   Purpose

   Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287L) and mCherry in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Lentiviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Pro287Leu

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSCV-FLAG-2xSTREP-CCND3(P284L)-Puro

   Plasmid

   #172617

   Purpose

   Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3(P284L) and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND3 (CCND3 Human)
   

   Use

   Retroviral

   Tags

   FLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Pro284Leu

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCV-FLAG-2xSTREP-CCND2(P281L)-Puro

   Plasmid

   #172624

   Purpose

   Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2(P281L) and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND2 (CCND2 Human)
   

   Use

   Retroviral

   Tags

   FLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Pro281Leu

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. px458-AMBRA1 (optimized for N-terminal knock-in)

   Plasmid

   #172608

   Purpose

   Expresses a gRNA against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP. This gRNA is suitable for the N-terminal knock-in of AMBRA1.

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   gRNA against AMBRA1 (AMBRA1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTRIPZ-p21-HA(RxL)

   Plasmid

   #215111

   Purpose

   Expresses p21-HA(RxL) in mammalian cells from an inducible lentiviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CDKN1A (CDKN1A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   p21(RxL) denotes mutations: R19A, R20A, L21A, F22…

   Promoter

   CMV (Tet inducible)

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

   Plasmid

   #108362

   Purpose

   Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans

   Depositor

   Luisa Cochella

   Article

   Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

   Inserts

   Ath-HEN1 (HEN1 Mustard Weed, Codon optimised for C. elegans)
   mCherry
   his-15 (his-15 Nematode)
   unc-54 3' UTR
   

   Tags

   2xFlag, MYC, T2A peptide, and TY1 linker

   Expression

   Worm

   Promoter

   A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…

   Available Since

   May 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLIB_3xFlag-His-nsp5CS-nsp9 (SARS-CoV-2)

   Plasmid

   #169177

   Purpose

   Baculoviral transfer vector to express SARS-CoV-2 nsp9 with nsp5-cleavable 3xFlag-His6 tag in insect cells

   Depositor

   John Diffley

   Article

   Milligan et al Biochem J. 2021 Jul 16;478(13):2499-2515

   Insert

   3xFlag-His-nsp5CS-nsp9 (ORF1ab SARS-CoV-2)
   

   Tags

   3xFlag-His6 (nsp5-cleavable)

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRIPZ-p21-HA(dPCNA)

   Plasmid

   #215112

   Purpose

   Expresses p21-HA(dPCNA) in mammalian cells from an inducible lentiviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CDKN1A (CDKN1A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   p21(dPCNA) denotes mutations M147A, D149A, F150A.

   Promoter

   CMV (Tet inducible)

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p21(dPCNA)-mCerulean-NeoR

    Plasmid

    #215082

    Purpose

    Expressesp21(dPCNA)-mCerulean in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDKN1A (CDKN1A Human)
    

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    p21(dPCNA) denotes mutations M147A, D149A, F150A.

    Promoter

    CMV

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p21(RxL)-mCerulean-NeoR

    Plasmid

    #215081

    Purpose

    Expresses p21(RxL)-mCerulean in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDKN1A (CDKN1A Human)
    

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    p21(RxL) denotes mutations: R19A, R20A, L21A, F22A

    Promoter

    CMV

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p21(RxL)-mCerulean-PuroR

    Plasmid

    #215078

    Purpose

    Expresses p21(RxL)-mCerulean in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Retroviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    p21(RxL) denotes mutations: R19A, R20A, L21A, F22A

    Promoter

    LTR

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p21(dPCNA)-mCerulean-PuroR

    Plasmid

    #215079

    Purpose

    Expresses p21(dPCNA)-mCerulean in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Retroviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    p21(dPCNA) denotes mutations M147A, D149A, F150A.

    Promoter

    LTR

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FLAG-D1-IRES-SS-D2-IRES-HA-D3

    Plasmid

    #215119

    Purpose

    Expresses FLAG-Cyclin D1, SS-Cyclin D2 and HA-Cyclin D3 in mammalian cells from an inducible lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Inserts

    CCND1 (CCND1 Human)
    CCND2 (CCND2 Human)
    CCND3 (CCND3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG-tag, HA-tag, and SS (STREP-STREP)

    Expression

    Mammalian

    Promoter

    IRES and TREG3

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPD162.21

    Plasmid

    #27511

    Depositor

    Andrew Fire

    Article

    Zhang et al Dev Biol. 2010 Aug 15. 344(2):603-10.

    Insert

    pmyo-3::dsRed::let-858_3'UTR
    

    Expression

    Worm

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGC235

    Plasmid

    #19683

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Plim-7
    

    Use

    Gateway entry clone

    Available Since

    March 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGC193

    Plasmid

    #19686

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    hlh-12(R25K)
    

    Use

    Gateway entry clone

    Available Since

    March 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHZ149

    Plasmid

    #27509

    Depositor

    Andrew Fire

    Article

    Zhang et al Dev Biol. 2010 Aug 15. 344(2):603-10.

    Insert

    pREAR::DsRed::unc-54_3'UTR
    

    Expression

    Worm

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGC157

    Plasmid

    #19682

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Prpl-28
    

    Use

    Entry clone

    Expression

    Worm

    Available Since

    March 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHZ054

    Plasmid

    #27505

    Depositor

    Andrew Fire

    Article

    Zhang et al Dev Biol. 2010 Aug 15. 344(2):603-10.

    Insert

    pmyo-3::yfp::lin-14_3'UTR
    

    Expression

    Worm

    Available Since

    March 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge