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pCDH-CRY2-mCh-superPLDx30

Plasmid

#188988

Purpose

A lentiviral plasmid encoding CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type)

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

PLD

Tags

CRY2 and mCherry

Expression

Mammalian

Mutation

K109R, P245A, G328S, G381V, G429D

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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AA011

Plasmid

#215973

Purpose

Fragmid fragment: (N' terminus) for C>T base editing

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; BPNLS_v1.1; rAPOBEC1_v1.1; Linker_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA010

Plasmid

#216038

Purpose

Fragmid fragment: (C' terminus) for C>T base editing

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

UGI_v1.1; NpNLS_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA013

Plasmid

#215975

Purpose

Fragmid fragment: (N' terminus) for A>G base editing

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; BPNLS_v1.1; ABE8e_v1.1; Linker_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHygromycin (GB0211)

Plasmid

#68204

Purpose

Provides the CDS of the Hygromycin resistance gene as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Hygromycin resistance gene coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pLPC-NFLAG TRF1 deltaA deltaM

Plasmid

#16065

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Mutation

Deleted acidic domain (aa1-66) and myb domain (aa…

Available Since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pOPTG-Tau-RING

Plasmid

#233691

Purpose

Expression of Tau-RING protein in bacteria

Depositor

Leo James

Article

Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

Insert

pOPTG

Expression

Bacterial

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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GR A458T

Plasmid

#133410

Purpose

overexpression of a mutant human glucocorticoid receptor in mammalian cells

Depositor

Andrew Cato

Article

Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

Insert

NR3C1 (NR3C1 Human)

Expression

Mammalian

Mutation

A458T

Available Since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pAGH42

Plasmid

#107249

Purpose

Vipp1-mGFPmut3 (or vipp1-gfp) construct with spectinomycin resistance cassette downstream used to replace the native copy of Vipp1 in Synechocystis (sll0617).

Depositor

Erin O'Shea

Article

Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

Insert

vipp1-gfp

Use

Suicide vector for destined for double homologous…

Tags

mGFPmut3

Expression

Bacterial

Promoter

native promoter of sll0617

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pSEL1

Plasmid

#23011

Depositor

James Bowie

Article

Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.

Insert

DHFR (Dhfr Mouse)

Tags

6 His Tag (not in frame) and Xpress epitope (not …

Expression

Bacterial

Available Since

March 10, 2010

Availability

Academic Institutions and Nonprofits only
pMMK ENTR 2

Plasmid

#206261

Purpose

ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Type

Empty backbone

Use

Multimate/gateway entr 2

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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anxA9-GFP

Plasmid

#107198

Purpose

Expresses human annexin A9-GFP fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Article

Goebeler et al FEBS Lett. 2003 Jul 10;546(2-3):359-64.

Insert

annexin A9 (ANXA9 Human)

Tags

Green Fluorescent Protein

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk1b1

Plasmid

#13753

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk1b1 (MAPK8 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-CMP-PE-V1

Plasmid

#202769

Purpose

Expresses CMP-PE-V1 in mammalian cells for prime editing

Depositor

Kyoungmi Kim

Article

Park et al Genome Biol. 2021 Jun 3;22(1):170. doi:

Insert

CMP-PE-V1

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLVXT-mWnt3

Plasmid

#121934

Purpose

doxycycline-inducible mouse Wnt3

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Wnt3 (Wnt3 Mouse)

Use

Lentiviral

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pSws1-nfsB-CFP

Plasmid

#86375

Purpose

for zebrafish transgenesis, expression of nfsB-CFP, also expresses EGFP

Depositor

Rachel Wong

Article

Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

Insert

nitroreductase (nfsB bacteria)

Use

Zebrafish

Tags

ECFP

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pBTK599

Plasmid

#110600

Purpose

BTK Type 8 Bacterial origin (R6K suicide plasmid) for homologous recombination

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP dropout

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCW57.1-pDUXCca

Plasmid

#198236

Purpose

Lentivector with tet-inducible porcine DUXC (codon altered)

Depositor

Stephen Tapscott

Article

Nip et al Hum Mol Genet. 2023 Feb 2:ddad021. doi:

Insert

porcine DUXC (codon altered)

Use

Lentiviral

Expression

Mammalian

Mutation

codon altered

Promoter

TRE

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pHRi_GPa3b17 TCRα

Plasmid

#187601

Purpose

Lentiviral expression of hTCR alpha (GPa3b17) for low expression

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

GPa3b17 hTCR alpha (TRA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mHSP

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pHRi_GPa3b17 TCRβ

Plasmid

#187602

Purpose

Lentiviral expression of hTCR beta (GPa3b17) for low expression

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

GPa3b17 hTCR beta (TRB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mHSP

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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