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pCAG-Cas13d-T2A-GFP(1-10)

Plasmid

#224566

Purpose

Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and split GFP(1-10) reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

Cas13d-T2A-GFP(1-10)

Use

CRISPR

Tags

T2A-split GFP(1-10)

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-nucGFP11-3xControlgRNA

Plasmid

#224568

Purpose

Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

GFP11-H2B

Use

CRISPR

Tags

Histone H2B (nuclear localization)

Mutation

Codon optimized

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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aav-tnt-dnmrtfa-gfp

Plasmid

#165035

Purpose

AAV-based delivery of dnMRTFA-GFP into cardiomyocytes in vivo

Depositor

William Pu

Article

Guo et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

Insert

dnMRTFA

Use

AAV

Tags

GFP

Mutation

deletion of 100 N-terminal amino acids

Promoter

cTNT

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLVXPuro-epsilon-COP-GFP

Plasmid

#66604

Purpose

Expresses epsilon COP as a GFP fusion

Depositor

David Stephens

Article

Morohashi et al Biochem J. 2010 Apr 14;427(3):401-12. do

Insert

epsilon COP (COPE Bovine)

Use

Lentiviral

Expression

Mammalian

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
YFP-DAXX-mSIM1

Plasmid

#134524

Purpose

Expresses YFP-DAXX fusion protein with a mutation in its 5' SIM

Depositor

Susan Janicki

Article

Shastrula et al J Cell Sci. 2019 Mar 26;132(6). pii: jcs

Insert

DAXX-mSIM1

Tags

YFP

Expression

Mammalian

Mutation

SIM (IIVL) 7-10 aa changed to IGAG

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-TIS11B CPM2

Plasmid

#121143

Purpose

Mammalian expression of mCherry tagged TIS11B CPM2 (charge pattern mutant 2)

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

ZFP36L1 (TIS11B) charge pattern mutant 2 (VEGFA Human)

Expression

Mammalian

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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AA302

Plasmid

#216052

Purpose

Fragmid fragment: (C' terminus) Nucleoplasmin NLS

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

NpNLS_v1.2; Stop; polyA_v1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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lentiGuide-RRBP1-2

Plasmid

#92157

Purpose

CRISPR guide RNA targeting human RRBP1

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

RRBP1 sgRNA-2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pGK:HygroR-CMV:mTagBFP2-A_UTR

Plasmid

#68479

Purpose

Lentivirus expressing mTagBFP2 with UTR sensor cassette and hygromycin resistance

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Hygromycin Resistance
mTagBFP2-A_UTR

Use

Lentiviral

Expression

Mammalian

Mutation

shRNA sensor cassette "A" added to 3&#x…

Promoter

CMV and pGK

Available Since

Nov. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-GFP-SINTBAD

Plasmid

#216840

Purpose

Stably express FKBP-GFP-tagged SINTBAD for CID induced mitophagy

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

SINTBAD (TBKBP1 Human)

Use

Lentiviral

Tags

FKBP-GFP

Expression

Mammalian

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-Cas13d-T2A-Citrine

Plasmid

#224565

Purpose

Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and Citrine reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

Cas13d-T2A-Citrine

Use

CRISPR

Tags

T2A-Citrine

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-H2B-mRuby3-IRES-eGtACR1-ST

Plasmid

#108960

Purpose

Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

eGtACR1-ST

Expression

Mammalian

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pUC118-mCtnnb1-FLIP-Puro

Plasmid

#84540

Purpose

CRISPR - FLIP targeting vector for mouse beta-catenin

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

Insert

mouse beta-catenin FLIP - Puro targeting vector

Use

Cre/Lox and Mouse Targeting

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-GFP-SF3B6-Delete-NLS-FLAG

Plasmid

#86871

Purpose

To express SF3B6 delete NLS signal with a GFP and FLAG tags at N- and C-terminus, respectively.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

SF3B6 (SF3B6 Human)

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

amino acids 7-29 deletion

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-Ift52

Plasmid

#128875

Purpose

For mammalian expression of mouse Ift52

Depositor

Aimin Liu

Article

Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

Insert

Ift52 (Ift52 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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mRIP3 GFP D161N

Plasmid

#41383

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (Ripk3 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Kinase Domain Mutant (D161N)

Promoter

CMV

Available Since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only
AA009

Plasmid

#216037

Purpose

Fragmid fragment: (C' terminus) Nucleoplasmin NLS

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

NpNLS_v1.1; 3xHA_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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XE122 XDsh delta DIX-GFP-CS2+

Plasmid

#16787

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1999 Jul 26;146(2):427-37.

Insert

Xdsh-delta DIX (dvl2.L Frog)

Use

Xenopus expression

Tags

gfp

Mutation

deletion of aa 1-159

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA_RGG-RFP-RGG

Plasmid

#124934

Purpose

Expression of RGG-RFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLuciferase (GB0096)

Plasmid

#68201

Purpose

Provides the CDS of the firefly luciferase protein as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Luciferase coding region

Use

Luciferase and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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