We narrowed to 10,224 results for: yeast
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Plasmid#197285PurposeShuttle plasmid that replicates in E. coli, S. cerevisiae, and A. laidlawii. Contains the OriC from A. laidlawii 8195DepositorInsertOrigin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions
UseSynthetic Biology; Bacterial and yeast cloningAvailable SinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMTS-hFluc
Plasmid#177716PurposeFirefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targetingDepositorInserthFluc
TagsMitochondrial Targeting Sequence MTS SSExpressionBacterial, Mammalian, and Y…Available SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWKS11
Plasmid#139418PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mNeonGreen marker.DepositorInsertmNeonGreen
ExpressionYeastPromoterTEF1Available SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWKS4
Plasmid#139411PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mCherry marker.DepositorInsertmCherry
ExpressionYeastPromoterTEF1Available SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZH82 pET28a-Rai1
Plasmid#231651PurposeBacterial expression vector expressing yeast S. cerevisiae rai1, no tagDepositorInsertrai1 (RAI1 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAT253
Plasmid#40943DepositorInsertGFP-LacI**
TagsGFP(S65T, V163A) and SV40 NLSExpressionYeastMutationLacI**/LacI2G(4 mutations) (see comment below); l…PromoterHis3 promoter fragmentAvailable SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBHM2404
Plasmid#173441PurposepRS316-mNeonGreen-CBP-2XFLAG-NAT1, contains C. neoformans optimized mNeonGreen and NAT1 marker for use as PCR templateDepositorInsertmNeonGreen-CBP-2XFLAG-NAT1
TagsCBP-2xFLAGExpressionBacterial and YeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBHM2406
Plasmid#173442PurposepRS316-mNeonGreen-CBP-2XFLAG-HYGB, contains C. neoformans optimized mNeonGreen and HYG marker for use as PCR templateDepositorInsertmNeonGreen-CBP-2XFLAG-HYGB
TagsCBP-2xFLAGExpressionBacterial and YeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCE41
Plasmid#174433PurposeC. auris LEUpOUT HYG marker gRNA expression construct. Use with pCE38 CAS9 expression construct.DepositorInsertHYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
UseCRISPRExpressionBacterial and YeastAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMJA179
Plasmid#128537PurposeExpresses anti-Prunus dulcis scFv AbDepositorInsertscFv Ab clone PD1F6
ExpressionYeastPromoterAOX1Available SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM17
Plasmid#210569PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Bpro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastPromoterSpH2BproAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTA-HUH
Plasmid#221152PurposeHisG-URA3-HisG recycling type marker in pTAs for Yarrowia lipolyticaDepositorInsertHisG-URA3-HisG
ExpressionBacterial and YeastAvailable SinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWKS9
Plasmid#139416PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mTurquoise2 marker.DepositorInsertmTurquoise2
ExpressionYeastPromoterTEF1Available SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYN2_1
Plasmid#184757PurposePlasmid for genome editing by CRISPR/Cas9DepositorInsertsCas9
sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
UseSynthetic BiologyTags2xNLSExpressionBacterial and YeastPromoterPGK1 and tRNA-Phe-HDV RibozymeAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM18
Plasmid#135484PurposeAgrobacterium T-DNA with SpunH2Bpr driving hph-tdTomatoDepositorInsertSpunH2Bpr hph-tdTomato ADH1t
TagstdTomatoExpressionBacterial and YeastPromoterSpunH2BprAvailable SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
p426MET25_SSKar2-sfpHluorin-HDEL (ER-sfpHluorin, MRV66)
Plasmid#115698PurposeER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiaeDepositorInsertSSKar2-sfpHluorin-HDEL
TagsER retention signal containing HDEL and ER signal…ExpressionYeastMutationfor introduced mutations see publicationPromoterMET25Available SinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEasy Flox
Plasmid#11725DepositorInsertpEasyFlox
UseCre/LoxAvailable SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM11
Plasmid#135483PurposeAgrobacterium T-DNA with SpunH2Bpr driving hph-GFPDepositorInsertSpunH2Bpr hph-GFP ADH1t
TagsGFPExpressionBacterial and YeastPromoterSpunH2BprAvailable SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEK19
Plasmid#210578PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdBIP1pro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastPromoterBdBIP1proAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIA-CEN-FLAG-LINXa4(RVH)-Bre1
Plasmid#116885PurposeFLAG-tagged LINXa4-Bre1 with RVH hinge-loop mutationsDepositorInsertLINXa4(RVH)-Bre1
UseSynthetic BiologyTagsFLAGExpressionYeastMutationBre1 point mutations K8A, K9A and K11A, LINXa3 wi…PromoterADH1Available SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only