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MAC_N_STA5A

Plasmid

#167799

Purpose

Expresses STA5A in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

STA5A (STAT5A Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pSB-CMV-MCS-Puro cPLA2-mK2-P2A-EGFP-LaminB1

Plasmid

#162566

Purpose

Used for the dual color labeling of cPLA2 and LaminB1 expression.

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Inserts

cytosolic phospholipase a2 (pla2g4aa Zebrafish)
Lamin B1 (lmnb1 Zebrafish)

Tags

EGFP, P2A, and mKate2

Expression

Mammalian

Promoter

CMV and SV40

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti6.2_3xFLAG_V5_ eGFP_V5

Plasmid

#160111

Purpose

Expresses eGFP in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

eGFP

Use

Lentiviral

Tags

3xFLAG, V5

Expression

Mammalian

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-BLRR

Plasmid

#158958

Purpose

Bioluminescent repair reporter (BLRR) for DNA double strand break (DSB) repairs: homology-directed repair (HDR) and non-homologous end joining (NHEJ).

Depositor

Charles P. Lai

Article

Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

Insert

BLRR

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti6.2_3xFLAG_V5_ mmDdx58_V5

Plasmid

#160108

Purpose

Expresses murine Ddx58 in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Ddx58 (Rigi Mouse)

Use

Lentiviral

Tags

3xFLAG, V5

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/GFP-ZNF598

Plasmid

#141191

Purpose

Expresses GFP-ZNF598 in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

ZNF598 (ZNF598 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST mpeg1:tomato-CAAX

Plasmid

#135215

Purpose

Red tagged macrophages

Depositor

David Tobin

Article

Oehlers et al Nature. 2015 Jan 29;517(7536):612-5. doi

Insert

mpeg1 and tdTomato

Use

Zebrafish expression

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Blast-DNTGFBR2-HA

Plasmid

#130888

Purpose

Expresses dominant negative mutant human TGFBR2

Depositor

Gian-Paolo Dotto

Article

Bordignon et al Cell Rep. 2019 Aug 27;28(9):2358-2372.e6

Insert

TGFBR2 (TGFBR2 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

Dominant Negative receptor lacking cytosolic cata…

Promoter

CMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-DAPK1

Plasmid

#116727

Purpose

Lentiviral expression of DAPK1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

DAPK1 (DAPK1 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-NRAS-Q61K

Plasmid

#116444

Purpose

Lentiviral expression of NRAS Q61K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

NRAS (NRAS Human)

Use

Lentiviral

Mutation

Q61K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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CYFIP1.mCherry

Plasmid

#122051

Purpose

Expresses human CYFIP1 in mammalian cells.

Depositor

Josef Kittler

Article

Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

Insert

CYFIP1 (CYFIP1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-OGDH_wobble

Plasmid

#110938

Purpose

OGDH ORF containing silent wobble mutations insensitive to corresponding shRNAs

Depositor

William Hahn

Article

Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

Insert

OGDH wobble cDNA (OGDH Human)

Use

Lentiviral

Mutation

15 silent wobble mutations corresponding to three…

Promoter

SV40

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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plenti-UbcP-FLAG-CRBN-pGK-HYG

Plasmid

#107374

Purpose

Lentiviral expression of FLAG-CRBN

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

cereblon (CRBN Human)

Tags

FLAG

Expression

Mammalian

Promoter

UbcP

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST JNKKTRmCerulean3

Plasmid

#90232

Purpose

Lentiviral vector to express JNK KTR mCerulean3 under PGK promoter (With Blasticidin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

JNK Kinase Translocation Reporter

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-HOXA5

Plasmid

#97040

Purpose

Expresses HOXA5 in mammalian cells

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Homeobox A5 (HOXA5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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HSPA8_pLX307

Plasmid

#98343

Purpose

Lentiviral expression of HSPA8

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

HSPA8 (HSPA8 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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MTCH2_pLX307

Plasmid

#98354

Purpose

Lentiviral expression of MTCH2

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

MTCH2 (MTCH2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pLIX-REST

Plasmid

#91896

Purpose

Doxycycline inducible expression of mouse REST

Depositor

Julien Sage

Insert

REST (Rest Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pAG303-Gal-GA50

Plasmid

#84907

Purpose

induce expression of dipeptide repeat GA in yeast

Depositor

Aaron Gitler

Article

Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

Insert

GA50 (C9orf72 Synthetic)

Tags

FLAG and Myc

Expression

Yeast

Promoter

GAL1

Available Since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 WAVE 2

Plasmid

#67098

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

WAVE 2 (WASF2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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